A novel coumarin-based fluorescent sensor for Ca2+ and sequential detection of F− and its live cell imaging

An Overview of Coumarin as a Versatile and Readily Accessible Scaffold with Broad-Ranging Biological Activities

“…Moreover, a linear relationship between the ratio of emission intensities at 532 and 465 nm and F − concentration over the range of 0–20 μΜ with a limit of detection of 0.025 μΜ was found [ 248 ]. Differently, Yao and co-workers exploited the capability of fluoride ions to form stable complex with Ca 2+ to design a novel fluorescent sensor ( 194 , Figure 61 ), synthesized from the combination of mandelic acid with 7-hydroxy-8-formylcoumarin through a hydrazine hydrate bridge, in order to selectively identify these two ionic species over other metal ions [ 249 ]. The fluorescence spectrum of compound 194 clearly increased when calcium ions were added to the solution with a limit of detection of 5.81 × 10 −7 M, while, once the complex between the probe and Ca 2+ ions was obtained, the addition of fluoride ions to the solution lead to the turn-off of the fluorescence response with a limit of detection 4.28 × 10 −7 M. Moreover, bio-imaging studies were performed in order to assure the possibility to exploit this novel chemosensor for the identification of Ca 2+ and F − ions in vivo, with positive outcome.…”

A Novel Reversible Fluorescent Probe for Sequential Detection of Aluminium Ion and PPi and Bioimaging in Living Cells

“…into the solution of HL, the fluorescence intensity was gradually increased (Figure a). The association constants (Ka) of probe HL toward Al 3+ was determined as 5.27×10 3 M −1 ( R 2 =0.9981, Figure b) on the basis of Benesi‐Hildebrand equation . In addition, the detection limit (DL) of HL towards Al 3+ was estimated for 4.96×10 −7 M (Figure s6) (3σ/S), which met the limit for drinking water according to the WHO standard (7.41 μM) .…”

Design and Applications of Small Molecular Probes for Calcium Detection

“…reportedahydroxycoumarin derived sensor 52 which showed fluorescencet urn-onr esponse upon complexation with Ca 2 + ions in aqueous medium ( Figure 17). [68] Sensor 52 binds to Ca 2 + ions by using 1:1s toichiometry with association constant value of 3.56 10 3 m À1 .T he binding mode of 52 to Ca 2 + ions involves the participation of hydroxyl, hydrazide and ether groups in addition to the N-atom of Schiff-base which was supported NMR and computational calculations. In another example, Zhang et al employed the mechanism of aggregation-induced emission (AIE) based on tetraphenylethylene (TPE) for the detection of Ca 2 + ions ( Figure 17).…”

“…Yao et al. reported a hydroxycoumarin derived sensor 52 which showed fluorescence turn‐on response upon complexation with Ca 2+ ions in aqueous medium (Figure ) . Sensor 52 binds to Ca 2+ ions by using 1:1 stoichiometry with association constant value of 3.56×10 3 m −1 .…”