A novel cotton WRKY gene, <i>GhWRKY6</i>‐like, improves salt tolerance by activating the ABA signaling pathway and scavenging of reactive oxygen species

Ullah, Abid; Sun, Heng; Hakim,; Yang, Xiyan; Zhang, Xianlong

doi:10.1111/ppl.12651

Cited by 144 publications

(82 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, our sequencing data indicated that two candidate genes encoding SOD, fifteen genes encoding POD and one gene encoding APX were obviously upregulated after the application of SA (Table S4). Considering that SOD, POD, and APX could function in the scavenging of ROS to improve salt tolerance [44], we speculated that moderate SA could mitigate the salt damage to cells by activating SOD, POD, and APX.…”

Section: Discussionmentioning

confidence: 99%

Salicylic Acid Alleviated Salt Damage of Populus euphratica: A Physiological and Transcriptomic Analysis

Rao

et al. 2019

Forests

View full text Add to dashboard Cite

Populus euphratica Oliv. is a model tree for studying abiotic stress, especially salt stress response. Salt stress is one of the most extensive abiotic stresses, which has an adverse effect on plant growth and development. Salicylic acid (SA) is an important signaling molecule that plays an important role in modulating the plant responses to abiotic stresses. To answer whether the endogenous SA can be induced by salt stress, and whether SA effectively alleviates the negative effects of salt on poplar growth is the main purpose of the study. To elucidate the effects of SA and salt stress on the growth of P. euphratica, we examined the morphological and physiological changes of P. euphratica under 300 mM NaCl after treatment with different concentrations of SA. A pretreatment of P. euphratica with 0.4 mM SA for 3 days effectively improved the growth status of plants under subsequent salt stress. These results indicate that appropriate concentrations of exogenous SA can effectively counteract the negative effect of salt stress on growth and development. Subsequently, transcripts involved in salt stress response via SA signaling were captured by RNA sequencing. The results indicated that numerous specific genes encoding mitogen-activated protein kinase, calcium-dependent protein kinase, and antioxidant enzymes were upregulated. Potassium transporters and Na+/H+ antiporters, which maintain K+/Na+ balance, were also upregulated after SA pretreatment. The transcriptome changes show that the ion transport and antioxidant enzymes were the early enhanced systems in response of P. euphratica to salt via SA, expanding our knowledge about SA function in salt stress defense in P. euphratica. This provides a solid foundation for future study of functional genes controlling effective components in metabolic pathways of trees.

show abstract

Section: Discussionmentioning

confidence: 99%

Salicylic Acid Alleviated Salt Damage of Populus euphratica: A Physiological and Transcriptomic Analysis

Rao

et al. 2019

Forests

View full text Add to dashboard Cite

show abstract

“…A similar result was found in cotton, the expression of GhWRKY6‐like was remarkably induced by ABA (Ullah et al . ). Genes encoding heat shock transcription factors, GATA, HY5, B3 domain transcription factors and trihelix DNA binding transcription factors were also changed in their expression levels after ABA treatment.…”

Section: Discussionmentioning

confidence: 97%

Global transcriptome analysis provides new insights in Thellungiella salsuginea stress response

Zhang

Shi

et al. 2019

Plant Biol J

View full text Add to dashboard Cite

Thellungiella salsuginea is highly tolerant to abiotic stress, while its a close relative Arabidopsis thaliana is sensitive to stress. This characteristic makes T. salsuginea an excellent model for uncovering the mechanisms of abiotic stress tolerance. Abscisic acid (ABA) plays essential roles in plant abiotic and biotic stress tolerance.• To test the changes in gene expression of T. salsuginea under ABA treatment, in this study, the transcriptomes of T. salsuginea roots and leaves were compared in response to exogenously application of ABA.• The results showed that ABA treatment caused different expression of 2,200 and 3,305 genes in leaves and roots, respectively, compared with the untreated control. In particular, genes encoding transcription factors such as WRKY, MYB, NAC, GATA, ethylene-responsive factors (ERFs), heat stress transcription factors, basic helix-loop-helix, PLATZ and B3 domain-containing family members were enriched. In addition, 49 and 114 differentially expressed genes were identified as ABA-regulated genes, separately in leaves and roots, respectively, which were related to biotic and abiotic stresses. The expression levels of some genes were validated by qRT-PCR. Different responses of genes to ABA treatment were discovered in T. salsuginea and A. thaliana.• This transcriptome analysis expands our understanding of the role of ABA in stress tolerance in T. salsuginea. Our study provides a wealth of information for improving stress tolerance in crop plants.Plant Biology 21 (2019) 796-804

show abstract

“…In the Gh4CL7 gene-silenced plants, the H 2 O 2 content was found to be increased significantly under drought stress, so was the MDA level, an indicator of ROS destructive effects [40]. SOD, POD, and CAT are antioxidant enzymes in plant cells which scavenge the toxic ROS and lead to enhanced tolerance under stress conditions [41]. We found that the activities of SOD, POD, and CAT were lower in the TRV:Gh4CL7 plants than in the TRV:00 plants indicating that the reduced ability of the Gh4CL7 silenced plants to scavenge ROS that might have led to membrane damages and chlorophyll content reduction.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Gh4CL gene family reveals a role of Gh4CL7 in drought tolerance

Sun

Xiong

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The function of 4-coumarate-CoA ligases (4CL) under abiotic stresses has been studied in plants, however, limited is known about the 4CL genes in cotton (G. hirsutum L.) and their roles in response to drought stress.Results: We performed genome-wide identification of the 4CL genes in G. hirsutum and investigated the expression profiles of the identified genes in various cotton tissues and in response to stress conditions with an aim to identify 4CL gene(s) associated with drought tolerance. We identified 34 putative 4CL genes in G. hirsutum that were clustered into three classes. Genes of the same class usually share a similar gene structure and motif composition. Many cis-elements related to stress and phytohormone responses were found in the promoters of the Gh4CL genes. Of the 34 Gh4CL genes, 26 were induced by at least one abiotic stress and 10 (including Gh4CL7) were up-regulated under the polyethylene glycol (PEG) simulated drought stress conditions. Virus-induced gene silencing (VIGS) in cotton and overexpression (OE) in Arabidopsis thaliana were applied to investigate the biological function of Gh4CL7 in drought tolerance. The Gh4CL7-silencing cotton plants showed more sensitive to drought stress, probably due to decreased relative water content (RWC), chlorophyll content and antioxidative enzyme activity, increased stomatal aperture, and the contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2). Arabidopsis lines overexpressing Gh4CL7, however, were more tolerant to drought treatment, which was associated with improved antioxidative enzyme activity, reduced accumulation of MDA and H2O2 and up-regulated stress-related genes under the drought stress conditions. In addition, compared to their respective controls, the Gh4CL7-silencing cotton plants and the Gh4CL7-overexpressing Arabidopsis lines had a ~20% reduction and a ~10% increase in lignin content, respectively. The expression levels of genes related to lignin biosynthesis, including PAL, CCoAOMT, COMT, CCR and CAD, were lower in Gh4CL7-silencing plants than in controls. Taken together, these results demonstrated that Gh4CL7 could positively respond to drought stress and therefore might be a candidate gene for improvement of drought tolerance in cotton.Conclusion: We characterized the 4CL gene family in upland cotton and revealed a role of Gh4CL7 in lignin biosynthesis and drought tolerance.

show abstract

A novel cotton WRKY gene, GhWRKY6‐like, improves salt tolerance by activating the ABA signaling pathway and scavenging of reactive oxygen species

Cited by 144 publications

References 63 publications

Salicylic Acid Alleviated Salt Damage of Populus euphratica: A Physiological and Transcriptomic Analysis

Salicylic Acid Alleviated Salt Damage of Populus euphratica: A Physiological and Transcriptomic Analysis

Global transcriptome analysis provides new insights in Thellungiella salsuginea stress response

Characterization of the Gh4CL gene family reveals a role of Gh4CL7 in drought tolerance

Contact Info

Product

Resources

About