A novel concept of the integrated fluorescence detection system and its application in a lab-on-a-chip microdevice

Mazurczyk, Radoslaw; Vieillard, Julien; Bouchard, Aude; Hannes, Benjamin; Krawczyk, Stanisław

doi:10.1016/j.snb.2006.04.069

Cited by 62 publications

(43 citation statements)

References 45 publications

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“…Depending on the substrate of choice, different methods can be used. Approaches reported in the literature include waveguide fabrication by silica on silicon [14][15][16][17], ion exchange in soda-lime glasses [18,19], photolithography in polymers [20,21] and liquid-core waveguides [22][23][24][25]. All these methods suffer, when applied to LOCs, from several limitations: (i) they are inherently planar techniques, i. e. they are able to define optical guiding structures only in two dimensions, close to the sample surface; (ii) they are multistep methods, involving multiple masking with critical alignments; (iii) they require cleanroom environment, and (iv) they typically create uneven surfaces which make sealing of the microfluidic channels problematic.…”

Section: Introductionmentioning

confidence: 99%

Femtosecond laser microstructuring: an enabling tool for optofluidic lab‐on‐chips

Osellame

Hoekstra

Cerullo

et al. 2011

Laser & Photonics Reviews

281

226

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This paper provides an overview of the rather new field concerning the applications of femtosecond laser microstructuring of glass to optofluidics. Femtosecond lasers have recently emerged as a powerful microfabrication tool due to their unique characteristics. On the one hand, they enable to induce a permanent refractive index increase, in a micrometer-sized volume of the material, allowing single-step, three-dimensional fabrication of optical waveguides. On the other hand, femtosecond-laser irradiation of fused silica followed by chemical etching enables the manufacturing of directly buried microfluidic channels. This opens the intriguing possibility of using a single laser system for the fabrication and three-dimensional integration of optofluidic devices. This paper will review the state of the art of femtosecond laser fabrication of optical waveguides and microfluidic channels, as well as their integration for high sensitivity detection of biomolecules and for cell manipulation.

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Section: Introductionmentioning

confidence: 99%

Femtosecond laser microstructuring: an enabling tool for optofluidic lab‐on‐chips

Osellame

Hoekstra

Cerullo

et al. 2011

Laser & Photonics Reviews

281

226

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“…• Integration of microtoroid whispering gallery mode (WGM) sensor into a microfluidic system for transporting molecules directly towards the most sensitive area of the sensor [5] • Combination of optical tweezers and microfluidic chip technologies based on dynamic fluid and dynamic light pattern, in which single and multiple laser traps are employed for cell transportation [12] • Fabrication of a reticulocyte microfluidic cytometer system based on optimized epifluorescence with the advantage in the signal-to-noise ratio [9] • All-optical and electrode-free approach into microfluidic chips for achieving reconfigurable dielectrophoresis (DEP) particle trapping [1] • A detective system by integration of fluorescence detector based on a microavalanche photodiode into a PDMS microfluidic device [3,4] • Implementing optical fibers to improve the performance of fluorescent spectroscopy detection on a portable chip [6] • A neuro-optical microfluidic platform to study mammalian individual axonal injury and subsequent regeneration [14] Key Research Findings…”

Section: Basic Methodologymentioning

confidence: 99%

Microfluidic Optical Devices

Çetin

Zeinali

2013

Encyclopedia of Microfluidics and Nanofluidics

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DefinitionMicrofluidic optical devices (MOD) are the emerging technology that combines today's microfluidics technology with the optics. However, MOD can be classified as the integration of these two technologies rather than combination of them. This integration provides a new approach for using microfluidics for control and manipulation of samples and optics for sensing. In this entry we propose a comprehensive review of emerging applications for microfluidic optical devices. OverviewIn many of the biological applications, microfluidics and optics technology have already been used in combination -microfluidics for control and manipulation of the samples and optics for sensing. Microelectromechanical systems (MEMS) and lab-on-a-chip communities try to embed optical devices into their microsystems to improve functionality of their devices. Presenting a comprehensive review of all the research in the field of microfluidic and optics integration is out the scope of this entry, but several numbers of representative examples of this integration are provided. Moreover, this integration results in handing:• Electric field induction by light exposure [1] • Integrated detection systems for microfluidic application (which increases the portability of the entire system) [3-5] • Sensitivity increase for the existing detection system [7,8] • Cell manipulation [9,11,12] • Small cell population sorting with high accuracy by tunable optical fibers [12] • Rapid detection of environmental contaminants [13] • A platform to study mammalian individual axonal injury [14] • Variable-focus liquid lens [15] Basic MethodologyLatest developments related to the combination of integrated optics and microfluidics have evolved towards device miniaturization with the ultimate goal of integrating many optical components onto a compact microchip, producing photonic integrated circuits with low cost and high degrees of

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“…If the masking layer presents a hydrophilic surface, the etching solution will easily penetrate through this crack and generate a pinhole. Many masking materials for wet etching of glass have been reported in the literature, including photoresist, 52 amorphous Si deposited at low (200 C) 53 or high temperatures (570 C), 54 LPCVD polysilicon, 55,56 Cr/Au, 57 Cr/photoresist, 58 bulk Si, 59 amorphous SiC/ PECVD, 60 Ag, 61 Mo, 62 and Ti. 63 A detailed analysis of masking materials used in wet etching of glass is presented in Ref.…”

Section: Wet Etchingmentioning

confidence: 99%

A practical guide for the fabrication of microfluidic devices using glass and silicon

Iliescu¹,

Taylor²,

Avram³

et al. 2012

Biomicrofluidics

298

207

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This paper describes the main protocols that are used for fabricating microfluidic devices from glass and silicon. Methods for micropatterning glass and silicon are surveyed, and their limitations are discussed. Bonding methods that can be used for joining these materials are summarized and key process parameters are indicated. The paper also outlines techniques for forming electrical connections between microfluidic devices and external circuits. A framework is proposed for the synthesis of a complete glass/silicon device fabrication flow. I. WHY USE SILICON AND GLASS IN MICRO-AND NANO-FLUIDIC APPLICATIONS?Micro-and nano-fluidic technology continues to be embraced by biologists, 1-3 chemists, 4 and engineers throughout academia and industry. As its applications have expanded, there has been an explosion in the range of materials and processes used to fabricate these devices. The earliest microfluidic devices (e.g., gas 5 and liquid 6 chromatography devices) were made from silicon and glass and borrowed processes directly from semiconductor and microelectromechanical systems (MEMS) manufacturing. 7 Now, however, many researchers have moved away from silicon and glass, and instead use cast elastomers such as polydimethylsiloxane (PDMS)-which is ideal for swift prototyping-or thermoplastic polymers, which can be hot-embossed or injection-molded and are well suited to inexpensive manufacturing. Yet, there remain many applications where glass and silicon offer advantages over polymeric materials. In this paper, we highlight these advantages and offer a framework for selecting fabrication processes when using silicon and glass. A. The dominance of soft lithographyOver the last decade, PDMS has become virtually the default material for forming microfluidic devices, 8 because of the sheer ease with which it can be cast on to a micro-scale mould and then strongly bonded to glass. 9 The elastomeric nature of the material has been exploited to integrate fluidic valves and pumps on-chip and has simplified the production of multi-layer devices because the soft layers readily conform to each other. 10,11 Yet, the low stiffness (usually <1 MPa) of PDMS relative to amorphous thermoplastics, silicon, and glass has its own a) Authors to whom correspondence should be addressed. Electronic addresses: ciliescu@ibn.a-star.edu.sg and hkt@ntu.edu.sg. 6, 016505 (2012) drawbacks. High aspect-ratio channels are notoriously difficult to fabricate in PDMS because of their propensity to collapse. 12 Moreover, difficulties in automating the handling of such a soft material have hampered efforts to scale up PDMS device manufacturing. Meanwhile, PDMS's high oxygen and water permeabilities have proved both a blessing and a curse in different applications.The hydrophobic nature of PDMS can be an important consideration for some biological applications. In drug screening applications, for example, hydrophobic drugs as well as metabolites (urea or albumin) can be absorbed into the device's material due to hydrophobic--hydrophobic interaction...

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A novel concept of the integrated fluorescence detection system and its application in a lab-on-a-chip microdevice

Cited by 62 publications

References 45 publications

Femtosecond laser microstructuring: an enabling tool for optofluidic lab‐on‐chips

Femtosecond laser microstructuring: an enabling tool for optofluidic lab‐on‐chips

Microfluidic Optical Devices

A practical guide for the fabrication of microfluidic devices using glass and silicon

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