A novel concept for scaffold-free vessel tissue engineering: Self-assembly of microtissue building blocks

Kelm, Jens; Lorber, Volker; Snedeker, Jess G.; Schmidt, Dörthe; Broggini-Tenzer, Angela; Weisstanner, Martin; Odermatt, Bernhard; Mol, Anita; Zünd, Gregor; Hoerstrup, Simon P.

doi:10.1016/j.jbiotec.2010.03.002

Cited by 159 publications

(130 citation statements)

References 22 publications

(26 reference statements)

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“…5,6 There are a variety of approaches currently used for the development of tissue-engineered blood vessels, including the use of cell-seeded degradable synthetic polymer scaffolds 2,3,7 and hydrogels, 8,9 as well as scaffold-free cellular self-assembly strategies. 1,4,10,11 Our laboratory developed a cellular self-assembly system to fabricate living engineered human vascular tissue constructs entirely from smooth muscle cells (SMCs). 10 Briefly, SMCs were seeded into annular agarose wells, where they aggregated and self-assembled to form tissue rings.…”

Section: Introductionmentioning

confidence: 99%

“…13 Cellular self-assembly may have advantages over scaffold-based approaches for vascular tissue engineering. Compared to cells seeded on scaffold materials, selfassembled cellular constructs may have greater cell density, enhanced extracellular matrix (ECM) production and tissue strength, improved biological function, and lower susceptibility to degradation and infection, 11,[14][15][16] and thus may be more similar in structure and function to native tissue. However, existing methods for fabricating self-assembled blood vessels create homogenous tubes not conducive to creating focal heterogeneities characteristic of certain diseases such as aneurysm or intimal hyperplasia.…”

Section: Introductionmentioning

confidence: 99%

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Cellular Self-Assembly with Microsphere Incorporation for Growth Factor Delivery Within Engineered Vascular Tissue Rings

Strobel

Dikina

Levi

et al. 2017

Tissue Engineering Part A

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Cellular self-assembly has been used to generate living tissue constructs as an alternative to seeding cells on or within exogenous scaffold materials. However, high cell and extracellular matrix density in self-assembled constructs may impede diffusion of growth factors during engineered tissue culture. In the present study, we assessed the feasibility of incorporating gelatin microspheres within vascular tissue rings during cellular selfassembly to achieve growth factor delivery. To assess microsphere incorporation and distribution within vascular tissue rings, gelatin microspheres were mixed with a suspension of human smooth muscle cells (SMCs) at 0, 0.2, or 0.6 mg per million cells and seeded into agarose wells to form self-assembled cell rings. Microspheres were distributed throughout the rings and were mostly degraded within 14 days in culture. Rings with microspheres were cultured in both SMC growth medium and differentiation medium, with no adverse effects on ring structure or mechanical properties. Incorporated gelatin microspheres loaded with transforming growth factor beta 1 stimulated smooth muscle contractile protein expression in tissue rings. These findings demonstrate that microsphere incorporation can be used as a delivery vehicle for growth factors within self-assembled vascular tissues.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cellular Self-Assembly with Microsphere Incorporation for Growth Factor Delivery Within Engineered Vascular Tissue Rings

Strobel

Dikina

Levi

et al. 2017

Tissue Engineering Part A

View full text Add to dashboard Cite

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“…A remarkable property of tissue spheroids is that maximal possible initial cell density can be achieved, which is essential for rapid fluid-solid transition, tissue assembly and maturation to maintain the morphological and compositional integrity of the newly fabricated construct. Kelm et al created living vessel tissues based exclusively on self-assembly (living cellular re-aggregates) of microtissues in an in vitro bioreactor in 2010 [54] . To provide the fragile tissue spheroids with necessary mechanical supports, rigid internal micro-scaffolds, or macro-porous carriers with micron-sized features were developed [55] , and proven to be effective in the cell protection during bioprinting processes.…”

Section: Bioinksmentioning

confidence: 99%

The trend towards in vivo bioprinting

Wang¹,

He²,

Liu³

et al. 2015

Int J Bioprinting

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Bioprinting is one of several newly emerged tissue engineering strategies that hold great promise in alleviating of organ shortage crisis. To date, a range of living biological constructs have already been fabricated in vitro using this technology. However, an in vitro approach may have several intrinsic limitations regarding its clinical applicability in some cases. A possible solution is in vivo bioprinting, in which the de novo tissues/organs are to be directly fabricated and positioned at the damaged site in the living body. This strategy would be particularly effective in the treatment of tissues/organs that can be safely arrested and immobilized during bioprinting. Proof-of-concept studies on in vivo bioprinting have been reported recently, on the basis of which this paper reviews the current state-of-the-art bioprinting technologies with a particular focus on their advantages and challenges for the in vivo application.

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“…Over the last 10 years, well-characterized cells either freshly isolated from tissue (primary cells) or cell lines were used as building blocks for co-culture systems or three-dimensional micro-tissues (22,23). An important step forward in this field was the development of permeable supports that allow researchers to keep the culture medium on either side of the cultured epithelium separate, leading to increased differentiation of the cultured cells (24) or the growth of different cell types on two sides of the membranes (25,26).…”

Section: Cell-based In Vitro Models: the Bottom Up Approachmentioning

confidence: 99%

In vitro-ex vivo model systems for nanosafety assessment

Wick

Chortarea

Guénat³

et al. 2015

European Journal of Nanomedicine

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Engineered nanomaterials have unique and novel properties enabling wide-ranging new applications in nearly all fields of research. As these new properties have raised concerns about potential adverse effects for the environment and human health, extensive efforts are underway to define reliable, cost-and time-effective, as well as mechanistic-based testing strategies to replace the current method of animal testing, which is still the most prevalent model used for the risk assessment of chemicals. Current approaches for nanomaterials follow this line. The aim of this review is to explore and qualify the relevance of new in vitro and ex vivo models in (nano) material safety assessment, a crucial prerequisite for translation into applications.

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A novel concept for scaffold-free vessel tissue engineering: Self-assembly of microtissue building blocks

Cited by 159 publications

References 22 publications

Cellular Self-Assembly with Microsphere Incorporation for Growth Factor Delivery Within Engineered Vascular Tissue Rings

Cellular Self-Assembly with Microsphere Incorporation for Growth Factor Delivery Within Engineered Vascular Tissue Rings

The trend towards in vivo bioprinting

In vitro-ex vivo model systems for nanosafety assessment

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