A novel, compact and efficient microchannel arrangement with multiple hydrodynamic effects for blood plasma separation

Prabhakar, Amit; Kumar, Y V Bala Varun; Tripathi, Siddhartha; Agrawal, Amit

doi:10.1007/s10404-014-1488-6

Cited by 45 publications

(71 citation statements)

References 42 publications

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“…Depending upon the hematocrit employed, and by varying the plasma channel resistance, the yield obtained in this particular design at flow rate of 0.5 ml/min is about 1 to 6%. The yield can be improved further by employing the ideas proposed by Prabhakar et al 39. Inertial based techniques for separation have shown promising values of yield.…”

Section: Resultsmentioning

confidence: 99%

“…Our microdevice exploits Zweifach-Fung bifurcation law at elevated dimensions383940 by manipulating the flow rate ratio at the bifurcation. The flow rate ratio was modified by suitably changing the resistance of the plasma and main blood outlet microchannels.…”

Section: Design Principlementioning

confidence: 99%

“…But, with an increase in the hematocrit content of the blood samples, there was a reduction in the separation efficiency of these microdevices. Our best design was able to provide separation efficiency around 80% with a yield of 3% using pure blood3940. However, extending these designs to achieve complete separation has not turned out to be trivial.…”

mentioning

confidence: 98%

“…Another issue with employing diluted blood samples in a plasma separation microdevice may be with respect to the quality of plasma extracted383940. Understandably, there is uncertainty associated with analyte detection from these diluted blood plasma samples.…”

mentioning

confidence: 99%

“…In our previous studies on ‘simple T’38 and ‘Tau’ microchannels3940, we presented several designs which provided excellent separation efficiency on moderate to high hematocrit content blood. But, with an increase in the hematocrit content of the blood samples, there was a reduction in the separation efficiency of these microdevices.…”

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confidence: 99%

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Microdevice for plasma separation from whole human blood using bio-physical and geometrical effects

Tripathi

Kumar

Agrawal

et al. 2016

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In this research work, we present a simple and efficient passive microfluidic device for plasma separation from pure blood. The microdevice has been fabricated using conventional photolithography technique on a single layer of polydimethylsiloxane, and has been extensively tested on whole blood and enhanced (upto 62%) hematocrit levels of human blood. The microdevice employs elevated dimensions of about 100 μm; such elevated dimensions ensure clog-free operation of the microdevice and is relatively easy to fabricate. We show that our microdevice achieves almost 100% separation efficiency on undiluted blood in the flow rate range of 0.3 to 0.5 ml/min. Detailed biological characterization of the plasma obtained from the microdevice is carried out by testing: proteins by ultra-violet spectrophotometric method, hCG (human chorionic gonadotropin) hormone, and conducting random blood glucose test. Additionally, flow cytometry study has also been carried on the separated plasma. These tests attest to the high quality of plasma recovered. The microdevice developed in this work is an outcome of extensive experimental research on understanding the flow behavior and separation phenomenon of blood in microchannels. The microdevice is compact, economical and effective, and is particularly suited in continuous flow operations.

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Section: Resultsmentioning

confidence: 99%

Section: Design Principlementioning

confidence: 99%

mentioning

confidence: 98%

mentioning

confidence: 99%

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confidence: 99%

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