A novel colorimetric PCR-based biosensor for detection and quantification of hepatitis B virus

Yang, Li; Du, Feng; Chen, Gangyi; Yasmeen, Aneela; Tang, Zhuo

doi:10.1016/j.aca.2014.05.032

Cited by 22 publications

(6 citation statements)

References 26 publications

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“…The hairpin structure of catalytic beacons can be opened through primer extension. ,− Xiao et al tethered a telomerase primer to one end of a hairpin caging a DNAzyme. The sequence, elongated by the telomerase, hybridized to the loop of the hairpin and opened the hairpin.…”

Section: Amplified Detection Of Nucleic Acids and Proteins Using Pero...mentioning

confidence: 99%

DNAzyme-Mediated Assays for Amplified Detection of Nucleic Acids and Proteins

et al. 2017

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Section: Amplified Detection Of Nucleic Acids and Proteins Using Pero...mentioning

confidence: 99%

DNAzyme-Mediated Assays for Amplified Detection of Nucleic Acids and Proteins

et al. 2017

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“…Because of the complete blocking and efficient releasing of G4-DNAzyme, the colorimetric method exhibited an excellent performance in PNK analysis with a low detection limit of 0.06 U/mL and a wide detection range from 0.06 to 100 U/mL. Tang and coworkers reported a strategy that applied G4-DNAzyme-containing hairpin into polymerase chain reaction (PCR) [119,120]. During the annealing step of PCR amplification, the hairpin DNA probe would open and form a stable duplex with one single-stranded PCR product.…”

Section: Peroxidase-mimicking Dnazyme (G4-dnazyme) As Colorimetricmentioning

confidence: 99%

Integrating Deoxyribozymes into Colorimetric Sensing Platforms

Chang

Zakaria

Deng

et al. 2016

Sensors

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Biosensors are analytical devices that have found a variety of applications in medical diagnostics, food quality control, environmental monitoring and biodefense. In recent years, functional nucleic acids, such as aptamers and nucleic acid enzymes, have shown great potential in biosensor development due to their excellent ability in target recognition and catalysis. Deoxyribozymes (or DNAzymes) are single-stranded DNA molecules with catalytic activity and can be isolated to recognize a wide range of analytes through the process of in vitro selection. By using various signal transduction mechanisms, DNAzymes can be engineered into fluorescent, colorimetric, electrochemical and chemiluminescent biosensors. Among them, colorimetric sensors represent an attractive option as the signal can be easily detected by the naked eye. This reduces reliance on complex and expensive equipment. In this review, we will discuss the recent progress in the development of colorimetric biosensors that make use of DNAzymes and the prospect of employing these sensors in a range of chemical and biological applications.

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“…The HBV can be found in blood and other body uids [3] The methods currently used for diagnosis of hepatitis B are performed through serological tests such as the enzyme-linked immunosorbent assay (ELISA), immuno uorescence and polymerase chain reaction (PCR) [4]. It is important to note that these assays require the use of reagents in high quantity, skilled labor and expensive equipment that prevents the implementation of these methodologies outside the laboratory environment [5].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Biosensor for Sensitive Detection of Hepatitis B in Human Plasma

Castro

Kochi

Flauzino

et al. 2022

Appl Biochem Biotechnol

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In this work we report the construction of an electrochemical device for molecular diagnosis of hepatitis B virus in the blood plasma of infected patients, using graphite electrodes functionalized with poly(4aminophenol) and sensitized with speci c DNA probe. The recognition of genomic DNA was evaluated by electrochemical techniques (DPV and EIS) and scanning electron microscopy. The genosensor was e cient in detecting genomic DNA with a linear range from 1.176 μg.ml -1 to 4.825 μg.ml -1 and detection limit of 35.69 ng.ml -1 (4.63 UI.ml -1 or 25.93 copies.ml -1 ), which is better than 10.00 UI.ml-1 limit of reference method, real-time PCR, used in point of care. EIS analysis shows that the genosensor resistence increased exponentially with the concentration of the genomic DNA target. The developed platform has inherent advantages to its applicability in real samples, such as good sensitivity, selectivity, and low sample volume, being interesting for application in diagnosis of hepatitis B virus in blood plasma.

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A novel colorimetric PCR-based biosensor for detection and quantification of hepatitis B virus

Cited by 22 publications

References 26 publications

DNAzyme-Mediated Assays for Amplified Detection of Nucleic Acids and Proteins

DNAzyme-Mediated Assays for Amplified Detection of Nucleic Acids and Proteins

Integrating Deoxyribozymes into Colorimetric Sensing Platforms

Electrochemical Biosensor for Sensitive Detection of Hepatitis B in Human Plasma

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