A novel colorimetric determination of free amino acids content in tea infusions with 2,4-dinitrofluorobenzene

Lin, Cheng; Chen, Qi; Zhang, Zhengzhu; Wan, Xiaochun

doi:10.1016/j.jfca.2008.08.007

Cited by 85 publications

(38 citation statements)

References 23 publications

(18 reference statements)

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“…These have often used reagents such as 2,4-dinitrofluorobenzene [2] and genipin [3]. Also, it has also been reported on the use of IR spectroscopy for the study of various extracts of Angelica [4].…”

Section: Sample Preparationmentioning

confidence: 99%

Peptide and Amino Acids Separation and Identification from Natural Products

Neda¹,

Vlăzan²,

Pop³

et al. 2012

Analytical Chemistry

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Section: Sample Preparationmentioning

confidence: 99%

Peptide and Amino Acids Separation and Identification from Natural Products

Neda¹,

Vlăzan²,

Pop³

et al. 2012

Analytical Chemistry

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“…Because amino acids microbial catabolism produces key flavour compounds in foods such as cheese, honey, beer and other fermented foodstuff [3,4], amino acids are commonly used in food technology and industry. Amino acids have a variety of roles in metabolism, such as building blocks of proteins, growth factors, metabolic regulators, second messengers and nutrients [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

High-performance liquid chromatographic method for determination of amino acids by precolumn derivatization with 4-chloro-3,5-dinitrobenzotrifluoride

Shi

Tang

Qian

et al. 2009

Analytica Chimica Acta

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“…The derivatization procedure was performed following the details provided in previous studies [15,16]. For the samples to be tested, 100 µl aliquots were added to a 1.5 ml plastic centrifugation tube, along with 200 µl of 0.1 M carbonate buffer and 100 µl of 0.1% DNFB (a 0.1 ml portion was dissolved in 100 ml of acetonitrile).…”

Section: Derivatization Proceduresmentioning

confidence: 99%

Detection of four different amino acid neurotransmitters in cultured rat neurons and the culture medium by precolumn derivatization high-performance liquid chromatography

Zhang

Wang

et al. 2016

NeuroReport

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We validated and used a high-performance liquid chromatography procedure for the determination of four different amino acid neurotransmitters in cultured rat neurons and used culture medium. Samples were derivatized using 2,4-dinitrofluorobenzene and the amino acids were separated on a C18 column. The method yielded good reproducibility and sensitivity for the quantification of the four free amino acid neurotransmitters, with average recovery factors of 80.25-118.43%, an intraday precision of 0.09-0.17%, and an interday precision of 0.62-0.74%. The assay method can be readily utilized as a precise, sensitive, and highly accurate method for the determination of concentrations of the four amino acid neurotransmitters in cultured rat neurons and used culture medium. Using the described methods, we found that the aspartate, glutamic acid, glycine, and γ-aminobutyric acid concentrations (µmol/g protein) in cultured rat neurons were 25.23±0.81, 35.16±0.32, 77.56±4.51, and 62.87±3.12, respectively, whereas their concentrations (µM) in the used culture medium were 18.18±0.82, 24.27±1.01, 107.18±9.56, and 35.78±2.98, respectively.

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A novel colorimetric determination of free amino acids content in tea infusions with 2,4-dinitrofluorobenzene

Cited by 85 publications

References 23 publications

Peptide and Amino Acids Separation and Identification from Natural Products

Peptide and Amino Acids Separation and Identification from Natural Products

High-performance liquid chromatographic method for determination of amino acids by precolumn derivatization with 4-chloro-3,5-dinitrobenzotrifluoride

Detection of four different amino acid neurotransmitters in cultured rat neurons and the culture medium by precolumn derivatization high-performance liquid chromatography

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