A novel colorimetric aptasensor using gold nanoparticle for a highly sensitive and specific detection of oxytetracycline

Kim, Yeon Seok; Kim, Joong Hyun; Kim, In Ae; Lee, Su Jin; Jurng, Jongsoo; Gu, Man Bock

doi:10.1016/j.bios.2010.08.046

Cited by 214 publications

(115 citation statements)

References 25 publications

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“…By reason of the aptamer-target interaction, aptamer desorps from gold nanoparticles then tightly couple OTA existed in the system. 24 The aggregation of AuNPs is caused by the existence of salt, leading to the solution color changing from red to blue (Fig. 3).…”

Section: Resultsmentioning

confidence: 99%

Aptamer-based Colorimetrie Biosensing of Ochratoxin A in Fortified White Grape Wine Sample Using Unmodified Gold Nanopartieles

et al. 2017

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Section: Resultsmentioning

confidence: 99%

Aptamer-based Colorimetrie Biosensing of Ochratoxin A in Fortified White Grape Wine Sample Using Unmodified Gold Nanopartieles

et al. 2017

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“…The minimum detection limit of TET by using this method was 10 nM, and the linear detection range was between 10 nM to 10 μM. The aptasensor showed higher selectivity for TET than the other structural analogs of OTC and DOX [99]. Recently, using ssDNA aptamers, Kim et al [100] developed an indirect competitive assay-based aptasensor for detection of OTC in milk, with the limit of detection 49.8 μg/L and recovery rate of more than 90 %.…”

Section: Antibioticsmentioning

confidence: 96%

Aptamer-Based Technology for Food Analysis

Liu

Zhang

2014

Appl Biochem Biotechnol

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Aptamers are short and functional single-stranded oligonucleotide sequences selected from systematic evolution of ligands by exponential enrichment (SELEX) process, which have the capacity to recognize various classes of target molecules with high affinity and specificity. Various analytical aptamers acquired by SELEX are widely used in many research fields, such as medicine, biology, and chemistry. However, the application of this innovative and emerging technology to food safety is just in infant stage. Food safety plays a very important role in our daily lives because varieties of poisonous and harmful substances in food affect human health. Aptamer technique is promising, which can overcome many disadvantages of existing detection methods in food safety, such as long detection time, low sensitivity, difficult, and expensive antibody preparation. This review provides an overview of various aptamer screening technologies and summarizes the recent applications of aptamers in food safety, and future prospects are also discussed.

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“…(62) Another versatile alternative is the light-based biosensors that include chemiluminescence, (63) fluorescence, (64) and colorimetric sensors. (65) Colorimetric biosensors have gained especial interest because of its simplicity: the results can be seen with naked eye without the need of an external apparatus. However, the sensitivity of this format is considerably lower than the previously reported.…”

Section: Aptamersmentioning

confidence: 99%

Selective Nanoparticles in Microextraction

Ríos-Gómez

Lasarte‐Aragonés

Cárdenas

et al. 2016

Encyclopedia of Analytical Chemistry

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Sorption capacity is a critical issue in microextraction techniques where the amount of sorbent is reduced to the low milligram range and even lower. In this context, the use of sorbents with a high superficial area is really desirable. Nanoparticles (NPs) fulfill this main requirement while, at the same time, provide the analyst with a wide variety of interaction chemistries that can be easily selected depending on the analytical problem under study. In addition, certain NPs present especial properties (e.g. superparamagnetism) that make them so attractive in the microextraction context. Focusing on sorption capacity is meaningless when complex samples, the usual situation in Bioanalysis, are processed as matrix components may overload the sorbent avoiding the extraction of the target analytes. In this scenario, selectivity then becomes a critical variable of concern. This article discusses the role of selectivity in microextraction and provides a general overview of the main coatings used to boost the selectivity of NPs. In particular, this article describes the use of two groups of coatings as selectivity enhancers. On the one hand, the use of synthetic polymers including molecularly imprinted polymers (MIPs) and restricted access materials (RAMs) is a well‐established alternative in analytical sciences. On the other hand, biopolymers, such as antibodies and aptamers, exploit biorecognition that is by far the most selective interaction. Both alternatives are described in detail in this article.

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A novel colorimetric aptasensor using gold nanoparticle for a highly sensitive and specific detection of oxytetracycline

Cited by 214 publications

References 25 publications

Aptamer-based Colorimetrie Biosensing of Ochratoxin A in Fortified White Grape Wine Sample Using Unmodified Gold Nanopartieles

Aptamer-based Colorimetrie Biosensing of Ochratoxin A in Fortified White Grape Wine Sample Using Unmodified Gold Nanopartieles

Aptamer-Based Technology for Food Analysis

Selective Nanoparticles in Microextraction

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