A Novel Collagen-Gelatin Scaffold for the Treatment of Deep Dermal Wounds—An Evaluation in a Minipig Model

Held, Manuel; Rahmanian-Schwarz, Afshin; Schiefer, Jennifer Lynn; Rath, Rebekka; Werner, Jan-Ole; Rahmanian, Shahab; Schaller, Hans-Eberhard; Petersen, Wiebke

doi:10.1097/dss.0000000000000727

Cited by 13 publications

(10 citation statements)

References 19 publications

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“…Stability and structural design are important determinants for skin tissue engineering, when constructing new dermal substitutes. In previous in vitro and in vivo studies, we already examined the innovative collagen-gelatin scaffold as a scaffold, and the results demonstrated an accelerated and improved dermal wound repair when applied as a single treatment, but even better when applied as multiple treatments [16,22,23]. Moreover, the manufacturing process of the scaffold allows the integration of bioactive substances such as antibiotics and growth factors.…”

Section: Discussionmentioning

confidence: 99%

The Topical Effect of rhGDF-5 Embedded in a Collagen–Gelatin Scaffold for Accelerated Wound Healing

et al. 2022

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The application of exogenous growth factors such as the recombinant human growth and differentiation factor 5 (rhGDF-5) represents a major research topic with great potential for the treatment of complex wounds. In a randomized, controlled minipig study, the topical effect of rhGDF-5 on full-thickness skin defects was evaluated. A total of 60 deep dermal wounds were either treated with rhGDF-5 embedded in an innovative collagen scaffold or another commonly used collagen matrix or left untreated. Wound healing was analyzed by planimetric analysis to determine wound closure over time. After 21 days, the areas of the initial wounds were excised, and the newly formed tissue was examined histologically. In comparison to untreated wounds, all examined matrices accelerated dermal wound healing. The largest acceleration of wound healing was seen with the high-dose rhGDF-5-treated wounds, which, compared to the untreated wounds, accelerated wound healing by 2.58 days, improved the neoepidermal thickness by 32.40 µm, and increased the epidermal cell density by 44.88 cells. The innovative collagen scaffold delivered rhGDF-5 adequately, served as a template to guide proliferating and restructuring cells, and accelerated wound healing. Thus, this composite product offers a novel tool for developing effective wound dressings in regenerative medicine.

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Section: Discussionmentioning

confidence: 99%

The Topical Effect of rhGDF-5 Embedded in a Collagen–Gelatin Scaffold for Accelerated Wound Healing

et al. 2022

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“…Topical application provides cell metabolism, migration, and differentiation. Moreover, it can stimulate extracellular matrix secretion and tissue regeneration [ 102 , 103 ]; therefore, shortening treatment time and improving the survival rate of transplants, with better clinical application value [ 104 , 105 , 106 ]. Although most topical applications were used in allogeneic stem cell therapy, other studies’ results were consistent with our systemic review and meta-analysis [ 78 , 107 ].…”

Section: Discussionmentioning

confidence: 99%

Autologous Stem Cell Therapy for Chronic Lower Extremity Wounds: A Meta-Analysis of Randomized Controlled Trials

Chiang

Chiu

Kang

et al. 2021

Cells

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Lower extremity chronic wounds (LECWs) commonly occur in patients with diabetes mellitus (DM) and peripheral arterial disease (PAD). Autologous stem cell therapy (ASCT) has emerged as a promising alternative treatment for those who suffered from LECWs. The purpose of this study was to assess the effects of ASCT on LECWs. Two authors searched three core databases, and independently identified evidence according to predefined criteria. They also individually assessed the quality of the included randomized controlled trials (RCTs), and extracted data on complete healing rate, amputation rate, and outcomes regarding peripheral circulation. The extracted data were pooled using a random-effects model due to clinical heterogeneity among the included RCTs. A subgroup analysis was further performed according to etiology, source of stem cells, follow-up time, and cell markers. A total of 28 RCTs (n = 1096) were eligible for this study. The pooled results showed that patients receiving ASCT had significantly higher complete healing rates (risk ratio (RR) = 1.67, 95% confidence interval (CI) 1.28–2.19) as compared with those without ASCT. In the CD34+ subgroup, ASCT significantly led to a higher complete healing rate (RR = 2.70, 95% CI 1.50–4.86), but there was no significant difference in the CD34− subgroup. ASCT through intramuscular injection can significantly improve wound healing in patients with LECWs caused by either DM or critical limb ischemia. Lastly, CD34+ is an important cell marker for potential wound healing. However, more extensive scale and well-designed studies are necessary to explore the details of ASCT and chronic wound healing.

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“…Next, a sterile semipermeable film was placed on top of every wound. 11 In terms of multiple applications, 6 minipigs received wound dressings on every second day. As the wounds were closing gradually, the size of the applied wound dressings was adapted to the wounds.…”

Section: Methodsmentioning

confidence: 99%

“…Wounds treated with a single application of the nonwoven scaffold 11 showed that the mean epidermal thickness was increased by …”

Section: Epidermal Thicknessmentioning

confidence: 99%

“…The evaluated nonwoven scaffold also provides a matrix structure, and it was shown in a previous study that it can accelerate wound closure and lead to a better skin quality in the healing of deep dermal wounds. 11 Before introducing new products to humans, animal studies allow clinical researchers to obtain more information in regard to application frequency or for dose finding. In this context, the effect of multiple applications of new gelatin-collagen nonwoven on deep dermal wounds was evaluated in comparison with the single application and an untreated control wound.…”

Section: Introductionmentioning

confidence: 99%

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Frequent Application of the New Gelatin-Collagen Nonwoven Accelerates Wound Healing

Schiefer

Rath

Held

et al. 2016

Advances in Skin &Amp; Wound Care

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A Novel Collagen-Gelatin Scaffold for the Treatment of Deep Dermal Wounds—An Evaluation in a Minipig Model

Cited by 13 publications

References 19 publications

The Topical Effect of rhGDF-5 Embedded in a Collagen–Gelatin Scaffold for Accelerated Wound Healing

The Topical Effect of rhGDF-5 Embedded in a Collagen–Gelatin Scaffold for Accelerated Wound Healing

Autologous Stem Cell Therapy for Chronic Lower Extremity Wounds: A Meta-Analysis of Randomized Controlled Trials

Frequent Application of the New Gelatin-Collagen Nonwoven Accelerates Wound Healing

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