A novel CLAVATA1 mutation causes multilocularity in Brassica rapa

Abstract

Locules are the seed-bearing structure of fruits. Multiple locules are associated with increased fruit size and seed set, and therefore control of locule number is an important agronomic trait. Locule number is controlled in part by the CLAVATA-WUSCHEL pathway. Disruption of either the CLAVATA1 receptor-like kinase or its ligand CLAVATA3 can cause larger floral meristems and an increased number of locules. In an EMS mutagenized population of Brassica rapa, we identified a mutant allele that raises the number of locules from 4 to a range of from 6 to 8. Linkage mapping and genetic analysis support that the mutant phenotype is due to a missense mutation in a CLAVATA 1 (CLV1) homolog. In addition to increased locule number, brclv1 individuals fail to terminate their floral meristems, resulting in internal gynoecia that negatively impact seed production.

Mapping a mutation causing pale yellow petals inBrassica rapa

“…The wsp mutation was created in the R-o-18 variety and the R500 variety was used for mapping. Ethyl methanesulfonate (EMS) treatment was performed as described in (Chow et al, 2022) and wsp was recovered in the M2 generation. Bulked Segregant Analysis sequencing was performed following (Chow et al, 2022).…”

“…Ethyl methanesulfonate (EMS) treatment was performed as described in (Chow et al, 2022) and wsp was recovered in the M2 generation. Bulked Segregant Analysis sequencing was performed following (Chow et al, 2022). The reannotated protein sequence was deposited in NCBI GeneBank (OP610629).…”