A Novel Class of Simple PCR Markers with SNP-Level Sensitivity for Mapping and Haplotype Characterization in Solanum Species

Syverson, Ryan L.; Bradeen, James M.

doi:10.1007/s12230-011-9191-6

Cited by 6 publications

(4 citation statements)

References 39 publications

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“…Further, sequencing and in silico approaches resulted in a scaffold upon which late blight resistance genes can be anchored. Now, single nucleotide polymorphisms (SNP) are representing the ultimate molecular marker type that continued to evolve today against P. infestans (Syverson and Bradeen ).…”

Section: Mapping and Cloning Of Resistance Genesmentioning

confidence: 99%

“…). In recent studies, Syverson and Bradeen () developed a set of markers for the RB gene using a mismatch amplification mutation assay (MAMA)‐PCR approach for tapping the potential of SNPs. Pajerowska‐Mukhtar et al.…”

Section: Marker‐assisted Selectionmentioning

confidence: 99%

“…In addition, several molecular markers were discussed earlier for the RB gene, which can be effective in selecting resistant genotypes ,b, Lokossou et al 2009). In recent studies, Syverson and Bradeen (2011) developed a set of markers for the RB gene using a mismatch amplification mutation assay (MAMA)-PCR approach for tapping the potential of SNPs. Pajerowska-Mukhtar et al (2009) identified nine SNPs associated with the allene oxide synthase 2 gene on chromosome 11, which likely controls late blight resistance.…”

Section: Marker-assisted Selectionmentioning

confidence: 99%

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Molecular markers for late blight resistance breeding of potato: an update

et al. 2013

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Late blight is the most devastating disease of the potato crop that can be effectively managed by growing resistant cultivars. Introgression of resistance (R) genes/quantitative trait loci (QTLs) from the Solanum germplasm into common potato is one of the plausible approaches to breed resistant cultivars. Although the conventional method of breeding will continue to play a primary role in potato improvement, molecular marker technology is becoming one of its integral components. To achieve rapid success, from the past to recent years, several R genes/QTLs that originated from wild/cultivated Solanum species were mapped on the potato genome and a few genes were cloned using molecular approaches. As a result, molecular markers closely linked to resistance genes or QTLs offer a quicker potato breeding option through marker-assisted selection (MAS). However, limited progress has been achieved so far through MAS in potato breeding. In near future, new resistance genes/QTLs are expected to be discovered from wild Solanum gene pools and linked molecular markers would be available for MAS. This article presents an update on the development of molecular markers linked to late blight resistance genes or QTLs by utilization of Solanum species for MAS in potato.Key words: late blight -molecular marker -MAS -potato -resistance gene -Solanum species Late blight caused by the oomycete (Phytophthora infestans (Mont.) de Bary) is the most important disease of potato production worldwide. This disease caused devastating impact on humanity in the mid-1840s when severe epidemics swept through Europe and resulted in the Irish potato famine (Fry 2008). Consequently, given its significant importance, there have been concerted global efforts for more than 100 years to develop durable resistant potato cultivars against P. infestans. However, evolution of new races of P. infestans was able to conquer the past resistance genes and resulted in susceptible cultivars worldwide. Durably resistant cultivars against a range of P. infestans isolates possessing multiple resistance genes are needed today, which can be developed in less time by conventional and molecular approaches. Regardless of the fact that common potato lacks significant sources of resistance, many wild Solanum species are rich sources of resistance genes. Globally breeders exploited only a very limited scale of Solanum biodiversity in potato breeding. For example, the genetic base of modern Indian potato cultivars is limited to 49 ancestors only involving the wild species S. rybinii and S. demissum. Late blight resistance genes were introgressed from the wild species S. demissum, S. stoloniferum and the cultivated S. tuberosum subsp. andigena and S. phureja into common potato in different parts of the world (Bradshaw et al. 2006c). Thus, it necessitates potato breeders to search for new sources of resistance in wild gene pools and their faster deployment into cultivars through markerassisted selection (MAS).Conventional breeding methods are of primary importance but ar...

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Section: Mapping and Cloning Of Resistance Genesmentioning

confidence: 99%

Section: Marker‐assisted Selectionmentioning

confidence: 99%

Section: Marker-assisted Selectionmentioning

confidence: 99%

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Molecular markers for late blight resistance breeding of potato: an update

et al. 2013

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“…This approach has led to the identification and filtration of a substantial number of SNP markers, ultimately resulting in the creation of a high-quality subset [ 67 ]. Syverson and Bradeen [ 81 ] mentioned that single nucleotide polymorphisms (SNPs) represent the ultimate molecular marker type that continued to evolve against P. infestans . DNA sequence-based markers like SNPs are the most common form of genomic DNA variation and are preferred over other genetic markers due to their low mutation rate, ease of use, and cost-effectiveness for genotyping [ 82 ].…”

Section: Genetic Methodologies For Breeding Resistance To P...mentioning

confidence: 99%

Modern Breeding Strategies and Tools for Durable Late Blight Resistance in Potato

Berindean,

Taoutaou,

Rida

et al. 2024

Plants

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Cultivated potato (Solanum tuberosum) is a major crop worldwide. It occupies the second place after cereals (corn, rice, and wheat). This important crop is threatened by the Oomycete Phytophthora infestans, the agent of late blight disease. This pathogen was first encountered during the Irish famine during the 1840s and is a reemerging threat to potatoes. It is mainly controlled chemically by using fungicides, but due to health and environmental concerns, the best alternative is resistance. When there is no disease, no treatment is required. In this study, we present a summary of the ongoing efforts concerning resistance breeding of potato against this devastating pathogen, P. infestans. This work begins with the search for and selection of resistance genes, whether they are from within or from outside the species. The genetic methods developed to date for gene mining, such as effectoromics and GWAS, provide researchers with the ability to identify genes of interest more efficiently. Once identified, these genes are cloned using molecular markers (MAS or QRL) and can then be introduced into different cultivars using somatic hybridization or recombinant DNA technology. More innovative technologies have been developed lately, such as gene editing using the CRISPR system or gene silencing, by exploiting iRNA strategies that have emerged as promising tools for managing Phytophthora infestans, which can be employed. Also, gene pyramiding or gene stacking, which involves the accumulation of two or more R genes on the same individual plant, is an innovative method that has yielded many promising results. All these advances related to the development of molecular techniques for obtaining new potato cultivars resistant to P. infestans can contribute not only to reducing losses in agriculture but especially to ensuring food security and safety.

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