A Novel cis-Acting Element in Promoters of Plant B-Type Cyclin Genes Activates M Phase-Specific Transcription

Ito, Masaki; Iwase, Masayuki; Kodama, Hiroaki; Lavisse, Philippe; Komamine, Atsushi; Nishihama, Ryuichi; Machida, Yasunori; Watanabe, Akira

doi:10.2307/3870592

Cited by 41 publications

(71 citation statements)

References 14 publications

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“…Whether functional properties of NtE2F resemble the E2F-1, 2 and 3 or E2F-4 and 5 subfamilies, along with whether there are related genes in plants, remains to be determined. Although Ito et al [15] reported that a M-speci¢c activator is a common cis-acting element that controls the M phasespeci¢c expression of cell cycle-related genes, the control of G1 and S phase-speci¢c expression is poorly understood in plants. In mammalian cells, the E2F/DP family of transcription factors is thought to play important roles in gene regulation during the S phase [7].…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of the E2F‐like gene in plants

et al. 1999

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The transcription factor E2F regulates the expression of genes involved in the progression of G1/S transition and DNA replication in mammalian cells. We cloned and characterized a cDNA (NtE2F) corresponding to a E2F homolog of tobacco (Nicotiana tabacum). The transcription of NtE2F was induced as cells progressed from G1 to the S phase and expressed much earlier than that of the proliferating cell nuclear antigen (PCNA) gene. We demonstrated that NtE2F can interact with the tobacco retinoblastoma (Rb)-related protein in a yeast two-hybrid assay. To further characterize NtE2F, the trans-activation activity of NtE2F was examined by using a transient assay in the tobacco Bright Yellow-2 (BY-2) cells with NtE2F fused to the DNAbinding domain of the yeast transcriptional activator GAL4. NtE2F activated the transcription of the L L-glucuronidase (GUS) reporter gene driven by a cauliflower mosaic virus (CaMV) 35S core promoter containing the GAL4-binding sequence. This is the first report of the identification of a functionally equivalent E2F-like gene in plants.z 1999 Federation of European Biochemical Societies.

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Section: Discussionmentioning

confidence: 99%

Isolation and characterization of the E2F‐like gene in plants

et al. 1999

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“…In our study, the cyclin B promoter was then variously modified, by deletion or point mutation, and assayed for its ability to direct phase-dependent expression of the luciferase reporter gene in BY2 cells (Ito et al 1998). This experiment identified an important cis element, called MSA element, which is repeated four times in the C. roseus cyclin B promoter.…”

Section: Targets Of Plant 3rmyb Factorsmentioning

confidence: 99%

“…These 3RMyb proteins were found to bind in vitro to the DNA sequences specified by the cis element with the core sequence of AACGG. This cis element is known as M phase-specific activator (MSA) element, and was first identified in tobacco (Ito et al 1998). cDNAs for two other 3RMyb proteins, NtmybC1 and NtmybC2, were isolated recently by PCR with the primers designed for amino acid sequences in the Myb domain that are highly conserved among 3RMyb proteins.…”

Section: Plant Myb Proteinsmentioning

confidence: 99%

Conservation and diversification of three-repeat Myb transcription factors in plants

Ito

2005

J Plant Res

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129

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The Myb family of transcription factors is characterized by the presence of a conserved DNA-binding domain called the Myb domain, which typically contains two or three imperfect repeat sequences. Within this family, Myb proteins containing three repeat motifs are evolutionarily conserved and have important roles in the cell cycle. Vertebrates have three Myb proteins, c-Myb, A-Myb, and B-Myb, all of which contain three repeats and are proposed to have a role at the G1/S transition. In plants, Myb proteins with three repeats are encoded by genes in a small subfamily within the large Myb gene family, most of which encode for Myb proteins with only two repeats. We have shown that Myb proteins with three repeats have an important role at the G2/M in tobacco, by regulating transcription of cyclin B genes and many other genes that are expressed at a similar time in the cell cycle. In this review, we summarize current knowledge on structure, function, and regulation of the plant Myb factors with three repeats, and discuss their conserved and divergent features in comparison with animal counterparts.

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“…1) were required to confer G 2 ͞M-specific expression in the context of the Ϫ205 promoter. Previous work had shown that this motif is required for G 2 ͞M phase expression in Catharanthus roseus cyclin CYM, when assayed in BY2 cells (17,34). The AACGG core MSA motif was mutated to ACTAG or AGCTG.…”

Section: Functional Analysis Of Elements In Synchronized By2 Cells Anmentioning

confidence: 99%

Arabidopsis TCP20 links regulation of growth and cell division control pathways

Potuschak²,

Colón‐Carmona³

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

305

287

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During postembryonic plant development, cell division is coupled to cell growth. There is a stringent requirement to couple these processes in shoot and root meristems. As cells pass through meristems, they transit through zones with high rates of cell growth and proliferation during organogenesis. This transition implies a need for coordinate regulation of genes underpinning these two fundamental cell functions. Here, we report a mechanism for coregulation of cell division control genes and cell growth effectors. We identified a GCCCR motif necessary and sufficient for high-level cyclin CYCB1;1 expression at G 2/M. This motif is overrepresented in many ribosomal protein gene promoters and is required for high-level expression of the S27 and L24 ribosomal subunit genes we examined. p33 TCP20 , encoded by the Arabidopsis TCP20 gene, binds to the GCCCR element in the promoters of cyclin CYCB1;1 and ribosomal protein genes in vitro and in vivo. We propose a model in which organ growth rates, and possibly shape in aerial organs, are regulated by the balance of positively and negatively acting teosinte-branched, cycloidea, PCNA factor (TCP) genes in the distal meristem boundary zone where cells become mitotically quiescent before expansion and differentiation.

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A Novel cis-Acting Element in Promoters of Plant B-Type Cyclin Genes Activates M Phase-Specific Transcription

Cited by 41 publications

References 14 publications

Isolation and characterization of the E2F‐like gene in plants

Isolation and characterization of the E2F‐like gene in plants

Conservation and diversification of three-repeat Myb transcription factors in plants

Arabidopsis TCP20 links regulation of growth and cell division control pathways

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