A novel chitosan–collagen-based hydrogel for use as a dermal filler: initial in vitro and in vivo investigations

Ma, Xiaoxuan; Deng, Jianjun; Du, Yuzhang; Li, Xian; Fan, Daidi; Zhu, Chenhui; Hui, Junfeng; Ma, Ping; Xue, Wenjiao

doi:10.1039/c3tb21842b

Cited by 50 publications

(34 citation statements)

References 32 publications

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“…Highly interconnected porous networks of materials can provide an environment suitable for nutrient transportation and cell growth and proliferation [33]. As shown in Figure 5A, nHD-1, nHD-2 and nHD-3 scaffolds all possessed evenly porous structures and good interpenetrating network structures.…”

Section: Structure Of the Scaffolds Before And After Enzymatic Degradmentioning

confidence: 99%

Fabrication of High-Strength and Porous Hybrid Scaffolds Based on Nano-Hydroxyapatite and Human-Like Collagen for Bone Tissue Regeneration

Liu

Fan

2020

Polymers

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A novel, three-dimensional, porous, human-like collagen (HLC)/nano-hydroxyapatite (n-HA) scaffold cross-linked by 1,2,7,8-diepoxyoctane (DEO) was successfully fabricated, which showed excellent mechanical and superior biological properties for bone tissue regeneration in this study. The physicochemical characterizations of different n-HA/HLC/DEO (nHD) scaffolds were investigated by determining the morphology, compression stress, elastic modulus, Young’s modulus and enzymatic hydrolysis behavior in vitro. The results demonstrated that nHD-2 and nHD-3 scaffolds showed superior mechanical properties and resistance to enzymatic hydrolysis compared to nHD-1 scaffolds. The cell viability, live cell staining and cell adhesion analysis results demonstrated that nHD-2 scaffolds exhibited low cytotoxicity and excellent cytocompatibility compared with nHD-1 and nHD-3 scaffolds. Furthermore, subcutaneous injections of nHD-2 scaffolds in rabbits produced superior anti-biodegradation effects and histocompatibility compared with injections of nHD-1 and nHD-3 scaffolds after 1, 2 and 4 weeks. In addition, the repair of bone defects in rabbits demonstrated that nHD-2 scaffolds presented an improved ability for guided bone regeneration and reconstruction compared to commercially available bone scaffold composite hydroxyapatite/collagen (HC). Collectively, the results show that nHD-2 scaffolds show promise for application in bone tissue engineering due to their excellent mechanical properties, anti-biodegradation, anti-biodegradation, biocompatibility and bone repair effects.

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Section: Structure Of the Scaffolds Before And After Enzymatic Degradmentioning

confidence: 99%

Fabrication of High-Strength and Porous Hybrid Scaffolds Based on Nano-Hydroxyapatite and Human-Like Collagen for Bone Tissue Regeneration

Liu

Fan

2020

Polymers

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“…Gene recombinant collagen‐based products are under research and development. In 2014, Ma et al . investigated a potential filler hydrogel which was comprised human‐like collagen and chitosan.…”

Section: Polymeric Injectable Fillersmentioning

confidence: 99%

Polymeric injectable fillers for cosmetology: Current status, future trends, and regulatory perspectives

Zhao

Zhang

et al. 2019

J of Applied Polymer Sci

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Injectable fillers have become a vital part of cosmetology. A wide variety of injectable fillers are developed for either correction of facial wrinkles and folds or facial contouring. As the most popular filler products, polymeric injectable fillers represent a class of the most common implantable medical devices. This review is intended to not only present current filler products, but also share perspectives of future trends, development processes, and regulatory considerations for the next generation of fillers. First, currently approved polymeric injectable fillers are described in detail. Second, future trends of polymeric injectable fillers are discussed. Fillers based on the emerging technologies in tissue engineering and regenerative medicine with stimuli‐responsive or tunable properties, as well as device/drug combination products, are promising candidates. Third, fillers for cosmetology are different from traditionally recognized medical devices. As a result, the target population of such fillers is different from that of other implantable devices, leading to different user needs that impact their development processes. Last, product applications, regulatory requirements, device evaluation, characteristics, and difficulties for regulating filler products for minimally invasive cosmetology are reviewed. Both regulatory science and scientific supervision are needed for polymeric injectable fillers which are facing great opportunities as well as challenges. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48515.

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“…6 The colorimetric assay was used to evaluate the cell viability based on increase of absorption at 450 nm aer adding CCK-8. 41 Cytotoxicity tests were performed before in vivo studies in order to explore if there is any potentially dangerous chemicals in the hydrogel.…”

Section: Cytotoxicity Analysis and Histomorphometry Near The Injectiomentioning

confidence: 99%

“…[1][2][3][4] Due to their high water content, tissue-mimic physical and mechanical properties and biocompatibility, hydrogels have the potency to be used in numerous biomedical applications such as tissue repair, reconstructive surgery, drug delivery and cell encapsulation. 5,6 Over the past decades, hydrogels have been extensively used in drug delivery and tissue engineering due to their multiple advantageous characteristics. [7][8][9][10][11][12][13][14] In addition, biodegradable hydrogels are specically attractive for tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Self-healing of thermally-induced, biocompatible and biodegradable protein hydrogel

Dong

Song

et al. 2016

RSC Adv.

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Serum albumin is the most abundant protein in the circulatory system to transport fatty acids, metabolites and drugs. In this study, a highly biocompatible protein hydrogel was prepared from bovine serum albumin (BSA) via thermal treatment. A circular dichroism study indicates that thermally-induced partial unfolding of the protein molecules exposes the buried hydrophobic groups in the core to the environment, thus leading to the formation of fine stranded 3-D networks. By controlling the heating temperature and protein concentration, the mechanical strength and structural stability of the as-prepared BSA hydrogel can be facilely manipulated. The moderate denaturation of the protein within the hydrogel system allows repetitive self-healing after damage when moderate heat was induced. The tensile strength and break strain of fully healed protein hydrogel were recovered to almost 100% of the original strength and elongation abilities. Additionally, the good biocompatibility of this hydrogel system was demonstrated through in vitro cytotoxicity analysis first. Furthermore, in vivo experiments using immunocompetent mice show that the subcutaneously injected hydrogel in mice can be fully degraded with negligible acute inflammatory response, indicating excellent in vivo biocompatibility. These features indicate that the as-developed self-healing protein hydrogel system with good biocompatibility and biodegradability holds great potential in the field of biomedical engineering.

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A novel chitosan–collagen-based hydrogel for use as a dermal filler: initial in vitro and in vivo investigations

Abstract: The biodegradability, histocompatibility and biocompatibility of injectable HCD hydrogels were determined throughin vitroandin vivotests.

Cited by 50 publications

References 32 publications

Fabrication of High-Strength and Porous Hybrid Scaffolds Based on Nano-Hydroxyapatite and Human-Like Collagen for Bone Tissue Regeneration

Fabrication of High-Strength and Porous Hybrid Scaffolds Based on Nano-Hydroxyapatite and Human-Like Collagen for Bone Tissue Regeneration

Polymeric injectable fillers for cosmetology: Current status, future trends, and regulatory perspectives

Self-healing of thermally-induced, biocompatible and biodegradable protein hydrogel

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