2013
DOI: 10.1371/journal.pone.0057318
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A Novel Cell-Penetrating Peptide Derived from Human Eosinophil Cationic Protein

Abstract: Cell-penetrating peptides (CPPs) are short peptides which can carry various types of molecules into cells; however, although most CPPs rapidly penetrate cells in vitro, their in vivo tissue-targeting specificities are low. Herein, we describe cell-binding, internalization, and targeting characteristics of a newly identified 10-residue CPP, denoted ECP32–41, derived from the core heparin-binding motif of human eosinophil cationic protein (ECP). Besides traditional emphasis on positively charged residues, the pr… Show more

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Cited by 41 publications
(49 citation statements)
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“…All amphiphiles showed concentration-dependent hemolysis at Ph 7.4. However, the hemolytic activity observed is markedly lower than the activity observed for similar molecules [40]. In fact, for a concentration of about 6 µM Xu et al [41] detected between 10% and 60% hemolysis while, at the same concentration, our amphiphiles reached between 7% and 20% hemolysis.…”
Section: Hemolytic Activity Studycontrasting
confidence: 62%
See 1 more Smart Citation
“…All amphiphiles showed concentration-dependent hemolysis at Ph 7.4. However, the hemolytic activity observed is markedly lower than the activity observed for similar molecules [40]. In fact, for a concentration of about 6 µM Xu et al [41] detected between 10% and 60% hemolysis while, at the same concentration, our amphiphiles reached between 7% and 20% hemolysis.…”
Section: Hemolytic Activity Studycontrasting
confidence: 62%
“…The design of these amphiphiles is new. The literature describes the importance of the coexistence of tryptophane and cysteine in the sequence of some CPP for cellular internalization [40], but there are no reports on amphiphilic amino acid derivatives which combine tryptophan, cysteine, and ornithine residues in the same structure. We believe that this combination could be the explanation for the fact that the lipoplexes formed from these amphiphiles are much more easily internalized in CHO-K1 cells than in other cell lines, which should be further researched.…”
Section: Gene Transfection Efficiencymentioning
confidence: 99%
“…Multiple mechanisms have been proposed for the internalization and/or penetration of cationic peptide into living cells, such as temperature/energy dependence (47), the involvement of proteoglycans (48,49), hydrophobicity (50), amphiphilicity (51), and distinct endocytotic pathways (52)(53)(54). The CTX homologues in the cobra venom are cationic polypeptide with different ligand-binding specificities and cellular targets.…”
Section: Discussionmentioning
confidence: 99%
“…Here a 10-residue peptide, covering major GAG binding motif of a human ribonuclease, is identified as a CPP AIF (anti-inflammatory CPP). CPP AIF has been shown to possess epithelial cell, GAG and lipid binding properties as well as cell penetrating activity through macropinocytosis [20,21]. Notably, CPP AIF is able to deliver small fluorescent molecules, recombinant proteins, nanoparticles, and peptidomimetic drugs into cells [20].…”
Section: Of 14mentioning
confidence: 99%