A novel cell membrane affinity sample pretreatment technique for recognition and preconcentration of active components from traditional Chinese medicine

Abstract

We describe a novel biomembrane affinity sample pretreatment technique to quickly screen and preconcentrate active components from traditional Chinese medicine (TCM), which adopts cell membrane coated silica particles (CMCSPs) as affinity ligands which benefit the biomembrane’s ability to maximize simulation of drug-receptor interactions in vivo. In this study, the prepared CMCSPs formed by irreversible adsorption of fibroblast growth factor receptor 4 (FGFR4) cell membrane on the surface of silica were characterized using different spectroscopic and imaging instruments. Drug binding experiments showed the excellent adsorption rate and adsorption capacity of FGFR4/CMCSPs compared with non-coated silica particles. The FGFR4/CMCSPs were used as solid-phase extraction sorbents to pretreat the TCM Aconitum szechenyianum Gay. The resultant FGFR4/CMCSPs exhibited good performance. In addition, high selectivity and recognition ability of the FGFR4/CMCSPs were determined by selectivity experiments. Four alkaloid were screened and identified, one of these alkaloid, napellonine, showed favorable anti-tumor activity in preliminary pharmacological verification trials including cell proliferation and molecular docking assays. The proposed cell membrane affinity sample pretreatment method is a reliable, effective and time-saving method for fast screening and enriching active compounds and can be extended to pretreat other TCMs as leading compounds resources.

Ligand fishing as a tool to screen natural products with anticancer potential

“…Based on the close relationship between FGFR4 receptors and cancer, Bu et al. [159] used a previously engineered HEK293 cell transfected with the Y367C mutation [160] to produce a mutant FGFR4‐expressing CM‐coated silica particle to screen a TCM product. It is important to note that the FGFR4 Y367C mutation is a dominant oncogene in MDA‐MB453 breast cancer cells [161] and as such, it is a good model for the screening of anticancer compounds through CM ligand fishing assay.…”

“…As a result, the authors fished out four alkaloids of which one (napellonine) had inhibitory action on FGFR4, with IC 50 of 133 μg/ml. Yet according to the authors, in comparison to the standard FGFR4 inhibitor PD173074 (IC 50 = 71.3 μg/ml), napellonine would need structural modifications to increase its potency towards FGFR4 [159].…”

Integrated Strategy of Network Pharmacological Prediction and Experimental Validation Elucidate Possible Mechanism of Bu-Yang Herbs in Treating Postmenopausal Osteoporosis via ESR1

“…The composition of traditional Chinese medicines is complex and diverse. Unlike Western medicine, which of the chemical compositions are simple and definite, the TCM formula is generally composed of complex matrixes and undefined active components which made it difficult to screen and analyze the exact bioactive components of the TCM formula ( Bu et al, 2017 ).…”

Determination of Cd2+, Cu2+, Mn2+, Pb2+, and Zn2+ in Aqueous Solution after Their Separation and Preconcentation as Metal - Dithizone Complexes on Activated Carbon

“…Thus, preconcentration methods capable of yielding high enrichment factors to bring the concentration levels of these metals within the scope of such instrument determination is required (Zhao et al, 2012;Zounr et al, 2018). Various methods of preconcentration techniques include solid-phase extraction (SPE), electrochemical methods (Yao et al, 2019), co-precipitation (Kagaya, 2016), membrane extraction (Bu et al, 2017), solvent extraction (Sorouraddin et al, 2020a) flame atomic absorption spectrometry (FAAS) (Er et al, 2013), graphite furnace atomic absorption spectrometry (GFAAS) (Soares & Nascentes, 2013), inductively coupled plasma-mass spectrometry (ICP-MS) (Javad et al, 2018) and inductively coupled plasma-atomic emission spectrometry (ICP-AES) (Pilarczyk et al, 2013), have been used for settling the above problems. Among these methods solid-phase extraction is considered the best one because of the low consumption of solvents, short time consuming, high preconcentration factor, safety in use and the technique of the work was easy and simple (Zhou et al, 2014).…”