A novel cell line established from caudal fin tissue of Carassius auratus gibelio is susceptible to cyprinid herpesvirus 2 infection with the induction of apoptosis

Lu, Jian-Fei; Xu, Dan; Lu, Liqun

doi:10.1016/j.virusres.2018.09.010

Cited by 30 publications

(26 citation statements)

References 32 publications

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“…Therefore, the EPC and FHM cell lines do not efficiently propagate CyHV-2. These results are consistent with previous studies (Lu, Xu, & Lu, 2018;Ma et al, 2015).…”

Section: Viral Isolation In Fhm and Epc Cell Linessupporting

confidence: 94%

“…Other studies have shown that when the Koi fin (Koi-Fin, KF) cell line was inoculated with diseased fish tissue homogenates, an obvious CPE was observed (Wang, 2012;Xu, Zeng, Yang, et al, 2013). However, most studies that have used the EPC, FHM, or KF cell line to isolate the virus have detected no CPE (Goodwin et al, 2006;Jeffery et al, 2007;Lovy & Friend, 2014;Lu et al, 2018;Ma et al, 2015;Shibata et al, 2015;Wang et al, 2013). In this study, the EPC and FHM cell lines were chosen to isolate the virus because we lacked a CyHV-2-sensitive cell line.…”

Section: Discussionmentioning

confidence: 99%

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First report of Cyprinid herpesvirus 2 outbreak in cultured gibel carp, Carassius auratus gibelio at low temperature

Ouyang

Zhou

Wang

et al. 2020

J World Aquaculture Soc

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Cyprinid herpesvirus 2 (CyHV-2) is the causative agent of herpesviral hematopoietic necrosis (HVHN), an infectious disease with high morbidity and mortality worldwide. Current research indicates that CyHV-2 only infects fish in the genus Carassius. Outbreaks of fish viral diseases are highly temperature-dependent, and HVHN outbreaks usually occur at water temperatures of 15-25 C. In November 2018, an infectious disease occurred on cultured gibel carp farms in ZiGong, Sichuan Province, China. The water temperature at the time was approximately 10 C. The clinical signs of the diseased fish included lethargy, inappetence, severe anoxia, hemorrhage on the body surface, and pale gills. Clinical and histopathological examinations, transmission electron microscopy (TEM), and molecular analyses were used to determine the etiology of this disease. Pathological observation revealed severe necrosis of the hematopoietic organs, particularly the gills, kidney, and spleen. Spherical herpesvirus-like virion particles were observed with TEM in Ping Ouyang and Yaojia Zhou contributed equally to this work.

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“…Therefore, the EPC and FHM cell lines do not efficiently propagate CyHV-2. These results are consistent with previous studies (Lu, Xu, & Lu, 2018;Ma et al, 2015).…”

Section: Viral Isolation In Fhm and Epc Cell Linessupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

First report of Cyprinid herpesvirus 2 outbreak in cultured gibel carp, Carassius auratus gibelio at low temperature

Ouyang

Zhou

Wang

et al. 2020

J World Aquaculture Soc

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“…Some studies reported that many different herpesviruses regulate apoptosis in infected cells (Miest et al, 2014;Scrochi et al, 2017;You et al, 2017). In our previous study, a novel cell line (Carassius auratus gibelio caudal fin cell line, GiCF) permission to CyHV-2 replication was established, and we demonstrated that CyHV-2 infection induces GiCF cell apoptosis (Lu et al, 2018a).…”

Section: Introductionmentioning

confidence: 78%

“…C. auratus gibelio caudal fin (GiCF) cell line was established in our previous work (Lu et al, 2018a), cells were grown in M199 medium (Gibco, USA) with 10% fetal bovine serum (Gibco, USA) and antibiotics (100 U penicillin ml −1 and 100 mg streptomycin ml −1 ) at 25°C. Hela cells were cultured in MEM Medium (Gibco, USA) supplemented with 10% fetal bovine serum (Gibco, USA) at 37°C and 5% CO 2 .…”

Section: Cells and Virusmentioning

confidence: 99%

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Cyprinid Herpesvirus 2 miR-C12 Attenuates Virus-Mediated Apoptosis and Promotes Virus Propagation by Targeting Caspase 8

Shen

et al. 2019

Front. Microbiol.

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DNA viruses, most notably members of the herpesvirus family, generally encode miRNAs to mediate both virus and host genes expression. We previously demonstrated that Cyprinid herpesvirus 2 (CyHV-2) encodes 17 miRNAs that are involved in innate immune signaling pathways. In this study, the function of CyHV-2-encoded miRNA was further investigated in GiCF cells. We found that miR-C4 promoted CyHV-2-induced apoptosis, while miR-C12 decreased CyHV-2-induced apoptosis. miR-C12 targeted to 3′ UTR sequence of caspase 8 and suppressed the expression of caspase 8. Besides, the silencing of caspase 8 by specific siRNA led to the attenuation of CyHV-2-induced apoptosis. Furthermore, caspase 8 was downregulated in cells transfected with miR-C12 during CyHV-2 infection. Overexpression of miR-C12 significantly suppressed CyHV-2induced apoptosis, while silencing of miR-C12 promoted CyHV-2-induced apoptosis. Finally, inhibition of miR-C12 resulted in suppression of CyHV-2 propagation, overexpression of miR-C12, and CASP8-siRNA-1 facilitated CyHV-2 propagation. Taken together, our results demonstrated that CyHV-2-encoded miR-C12 to suppress virus-induced apoptosis and promoted virus replication by targeting caspase 8.

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Immune gene expression in cyprinid herpesvirus-2 (CyHV-2)–sensitized peripheral blood leukocytes (PBLs) co-cultured with CyHV-2-infected goldfish fin cell line

et al. 2021

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Goldfish is one of the preferred ornamental fish which is highly susceptible to cyprinid herpesvirus-2 (CyHV-2) infection. The present study aimed to analyse immune gene expression in a co-culture of CyHV-2-sensitized goldfish peripheral blood leukocytes (PBLs) with CyHV-2-infected fantail goldfish fin cell lines (FtGF). Goldfish were sensitized with intraperitoneal TCID 50 dose (10 7.8±0.26 /mL) of CyHV-2. After 2 weeks, PBLs were collected and co-cultured with CyHV-2-infected FtGF cells keeping both uninfected FtGF cells and PBL control groups. After 2 days of co-culture, WST-1 assay for cell proliferation was performed at 450 nm during the 2nd, 4th and 6th days of coculture. The results showed a significant increase (p < 0.05) in cell density in CyHV-2infected PBL and virus-infected FtGF cells during the 4th day post co-culture which confirmed effector cell generation. Expressions of few immune genes were checked taking RNA samples of CyHV-2-induced PBLs post co-culture with infected FtGF cells along with uninfected FtGF cells as control group at different time periods (2nd, 4th and 6th days) in triplicate. The results indicated increased expression of CD8α, IFNγ, b2m, MHC I, LMP 7, IL-10, IL-12 and GATA3 except Tapasin. From the above study, we concluded that goldfish showed both Th1-and Th2-mediated immune responses to CyHV-2. The current findings support the scope for further vaccine development against CyHV-2 for goldfish.

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A novel cell line established from caudal fin tissue of Carassius auratus gibelio is susceptible to cyprinid herpesvirus 2 infection with the induction of apoptosis

Cited by 30 publications

References 32 publications

First report of Cyprinid herpesvirus 2 outbreak in cultured gibel carp, Carassius auratus gibelio at low temperature

First report of Cyprinid herpesvirus 2 outbreak in cultured gibel carp, Carassius auratus gibelio at low temperature

Cyprinid Herpesvirus 2 miR-C12 Attenuates Virus-Mediated Apoptosis and Promotes Virus Propagation by Targeting Caspase 8

Immune gene expression in cyprinid herpesvirus-2 (CyHV-2)–sensitized peripheral blood leukocytes (PBLs) co-cultured with CyHV-2-infected goldfish fin cell line

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