A novel canine lymphoma cell line: A translational and comparative model for lymphoma research

Kisseberth, William C.; Nadella, Murali V.P.; Breen, Matthew; Thomas, Rachael; Duke, Shannon E.; Murahari, Sridhar; Kosarek, Carrie E.; Vernau, William; Avery, Anne C.; Burkhard, Mary Jo; Rosol, Thomas J.

doi:10.1016/j.leukres.2007.04.003

Cited by 48 publications

(50 citation statements)

References 53 publications

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“…1 in this study with Fig. 3 of Kisseberth et al 2007). Kisseberth et al (2007) additionally confirmed the DNA copy number changes of four genes within alterations detected in both studies (P16, MYC, RB1, PTEN).…”

Section: Results Acgh Of a Canine Lymphoma Cell Linesupporting

confidence: 75%

“…(3) The cluster analysis of CNVrs tends to group breeds within their breed classes. Moreover, as we mentioned above, previous BAC aCGH and FISH analysis of the OSW cell line validated many of the large regions of copy number alteration and several specific affected genes (Kisseberth et al 2007). We confirmed eight of the copy number changes we discovered in our aCGH survey ( Table 2).…”

Section: Annotation and Validation Of Cnvsmentioning

confidence: 54%

“…3 of Kisseberth et al 2007). Kisseberth et al (2007) additionally confirmed the DNA copy number changes of four genes within alterations detected in both studies (P16, MYC, RB1, PTEN). Beyond validating our methods, we also identified alterations previously implicated in canine lymphoma, such as the whole chromosome amplification of chr 13 (containing MYC) and loss of chr 11 and regions of chr 22 (containing RB1) and chr 26 (containing PTEN) (Hahn et al 1994;Thomas et al 2003;Modiano et al 2007).…”

Section: Results Acgh Of a Canine Lymphoma Cell Linementioning

confidence: 97%

“…We began our study by conducting aCGH on the well-characterized OSW T-cell lymphoma cell line from an Airedale Terrier (Kisseberth et al 2007; Supplemental Table S1). The aCGH reference DNA for this hybridization-and for all others reported here-was from a pedigreed Boxer.…”

Section: Results Acgh Of a Canine Lymphoma Cell Linementioning

confidence: 99%

“…The aCGH reference DNA for this hybridization-and for all others reported here-was from a pedigreed Boxer. Notably, OSW was previously analyzed on a low resolution BAC array, and several copy number alterations were validated (Kisseberth et al 2007). Each spot of such BAC arrays is comprised of many fragments 0.5-2 kb in size, but our spots correspond to single unique-sequence oligos.…”

Section: Results Acgh Of a Canine Lymphoma Cell Linementioning

confidence: 99%

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Mapping DNA structural variation in dogs

Chen¹,

Swartz²,

Rush³

et al. 2008

Genome Res.

128

131

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DNA structural variation (SV) comprises a major portion of genetic diversity, but its biological impact is unclear. We propose that the genetic history and extraordinary phenotypic variation of dogs make them an ideal mammal in which to study the effects of SV on biology and disease. The hundreds of existing dog breeds were created by selection of extreme morphological and behavioral traits. And along with those traits, each breed carries increased risk for different diseases. We used array CGH to create the first map of DNA copy number variation (CNV) or SV in dogs. The extent of this variation, and some of the gene classes affected, are similar to those of mice and humans. Most canine CNVs affect genes, including disease and candidate disease genes, and are thus likely to be functional. We identified many CNVs that may be breed or breed class specific. Cluster analysis of CNV regions showed that dog breeds tend to group according to breed classes. Our combined findings suggest many CNVs are (1) in linkage disequilibrium with flanking sequence, and (2) associated with breed-specific traits. We discuss how a catalog of structural variation in dogs will accelerate the identification of the genetic basis of canine traits and diseases, beginning with the use of whole genome association and candidate-CNV/gene approaches.

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Section: Results Acgh Of a Canine Lymphoma Cell Linesupporting

confidence: 75%

Section: Annotation and Validation Of Cnvsmentioning

confidence: 54%

Section: Results Acgh Of a Canine Lymphoma Cell Linementioning

confidence: 97%

Section: Results Acgh Of a Canine Lymphoma Cell Linementioning

confidence: 99%

Section: Results Acgh Of a Canine Lymphoma Cell Linementioning

confidence: 99%

See 3 more Smart Citations

Mapping DNA structural variation in dogs

Chen¹,

Swartz²,

Rush³

et al. 2008

Genome Res.

128

131

View full text Add to dashboard Cite

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An Overview of Molecular Cancer Pathogenesis, Prognosis, and Diagnosis

Cullen

Breen

2016

Tumors in Domestic Animals

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Identification of altered MicroRNA expression in canine lymphoid cell lines and cases of B‐ and T‐Cell lymphomas

Uhl¹,

Krimer²,

Schliekelman³

et al. 2011

Genes Chromosomes & Cancer

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Canine lymphoma is a common spontaneous tumor with many similarities to human lymphoma, and thus has potential to be an important animal model of lymphomagenesis. This study determined that microRNA (miRNA) expression in canine tumors can be assessed using a commercially available human cancer miRNA qPCR array. miRNA expression in six different canine lymphoid cell lines and in naturally occurring canine B- and T-cell lymphomas was compared using RNA harvested from normal canine peripheral blood mononuclear cells (PBMC) and normal lymph nodes (LN) as controls. We found that false discovery rate (FDR) correction for multiple testing after quantile normalization controlled for variation across arrays and that they were the best methods for normalization and statistical analysis. Increases in miRNAs known to upregulate oncogenes (miR19a+b, miR17-5p) and decreased expression of miRNAs with tumor suppressor functions (miR-203, miR-218, and miR-181a) also seen in human lymphoid malignancies were observed. However, there were few similarities between canine groups. The results of this study indicate that the use of both PBMC and LN cells as controls provides different, but potentially equally important targets for further analysis. Our findings of miRNA dysregulation in canine lymphoid cell lines and clinical cases of lymphoma emphasize the potential of canine lymphoma as an important spontaneous, large animal model of human B- and T-cell lymphomas.

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A novel canine lymphoma cell line: A translational and comparative model for lymphoma research

Cited by 48 publications

References 53 publications

Mapping DNA structural variation in dogs

Mapping DNA structural variation in dogs

An Overview of Molecular Cancer Pathogenesis, Prognosis, and Diagnosis

Identification of altered MicroRNA expression in canine lymphoid cell lines and cases of B‐ and T‐Cell lymphomas

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