A novel calmodulin-binding protein, belonging to the WD-repeat family, is localized in dendrites of a subset of CNS neurons.

Castets, Francis; Bartoli, Marc; Barnier, Jean-Vianney; Baillat, Gilbert; Salin, Pascal; Moqrich, Aziz; Bourgeois, Jean-Pierre; Denizot, Franqois; Rougon, Geneviève; Calothy, Georges; Monneron, Ariane

doi:10.1083/jcb.134.4.1051

Cited by 118 publications

(171 citation statements)

References 43 publications

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“…The solubilized proteins were electrophoresed on 8, 10, or 15% polyacrylamide-SDS gels and transferred onto Protran membranes (Schleicher and Schuell). The antibodies used for revelation were the anti-striatin serum 75 (1:2000; Castets et al, 1996) and affinity-purified anti-phocein, anti-SG2NA, and anti-zinedin antibodies (all at 0.2-0.3 g/ml). The ECL procedure (Pierce) was used.…”

Section: Coimmunoprecipitation and Pull-down Assaysmentioning

confidence: 99%

“…L40 yeast strain cells grown in minimal medium were transformed with pLex-stri, using the lithium acetate method (Gietz et al, 1992). The Lex-stri fusion protein was stably expressed in L40 cells, as verified by immunoblotting using anti-striatin antibodies ( Castets et al, 1996). L40 cells expressing Lex-stri were transformed with the plasmid library.…”

Section: Two-hybrid Assaymentioning

confidence: 99%

“…A full-length striatin insert (Castets et al, 1996) was ligated into the pLex 11 vector (a gift from M.C. Dagher, Commissariat à l'Energie Atomique, Grenoble, France) in-frame with the LexA DNA-binding domain, yielding plasmid pLex-stri.…”

Section: Two-hybrid Assaymentioning

confidence: 99%

“…The structure and composition of axons and dendrites are quite different, particularly concerning their respective plasma membranes, cytoskeleton (Gunning et al, 1998;Baas, 1999), and proteins involved in vesicular traffic, such as motor proteins (Foletti et al, 1999;Burack et al, 2000). Striatin is a neuronal, intracellular protein strictly expressed in the somato-dendritic compartment, including spines, of subsets of neurons: thus, it can be considered as a marker of neuronal polarity (Castets et al, 1996;Kachidian et al, 1998;Salin et al, 1998). Found in the cytosol as well as associated with membranes, striatin is endowed with protein-protein association modules as diverse as a caveolin-binding motif, a coiled-coil structure, a Ca 2ϩ -calmodulin-binding amphiphilic helix, and a WD-repeat domain (Castets et al, 1996;Bartoli et al, 1998Bartoli et al, , 1999bMoqrich et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…Striatin is a neuronal, intracellular protein strictly expressed in the somato-dendritic compartment, including spines, of subsets of neurons: thus, it can be considered as a marker of neuronal polarity (Castets et al, 1996;Kachidian et al, 1998;Salin et al, 1998). Found in the cytosol as well as associated with membranes, striatin is endowed with protein-protein association modules as diverse as a caveolin-binding motif, a coiled-coil structure, a Ca 2ϩ -calmodulin-binding amphiphilic helix, and a WD-repeat domain (Castets et al, 1996;Bartoli et al, 1998Bartoli et al, , 1999bMoqrich et al, 1998). Striatin down-regulation in embryonic motoneurons leads to the blockade of dendritic, but not axonal, growth, indicating that it may play a role in the establishment of polarity within developing neurons (Bartoli et al, 1999a).…”

Section: Introductionmentioning

confidence: 99%

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Molecular Cloning and Characterization of Phocein, a Protein Found from the Golgi Complex to Dendritic Spines

Baillat

Moqrich

Castets

et al. 2001

MBoC

115

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Phocein is a widely expressed, highly conserved intracellular protein of 225 amino acids, the sequence of which has limited homology to the subunits from clathrin adaptor complexes and contains an additional stretch bearing a putative SH3-binding domain. This sequence is evolutionarily very conserved (80% identity between Drosophila melanogaster and human). Phocein was discovered by a yeast two-hybrid screen using striatin as a bait. Striatin, SG2NA, and zinedin, the three mammalian members of the striatin family, are multimodular, WD-repeat, and calmodulinbinding proteins. The interaction of phocein with striatin, SG2NA, and zinedin was validated in vitro by coimmunoprecipitation and pull-down experiments. Fractionation of brain and HeLa cells showed that phocein is associated with membranes, as well as present in the cytosol where it behaves as a protein complex. The molecular interaction between SG2NA and phocein was confirmed by their in vivo colocalization, as observed in HeLa cells where antibodies directed against either phocein or SG2NA immunostained the Golgi complex. A 2-min brefeldin A treatment of HeLa cells induced the redistribution of both proteins. Immunocytochemical studies of adult rat brain sections showed that phocein reactivity, present in many types of neurons, is strictly somato-dendritic and extends down to spines, just as do striatin and SG2NA.

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Section: Coimmunoprecipitation and Pull-down Assaysmentioning

confidence: 99%

Section: Two-hybrid Assaymentioning

confidence: 99%

Section: Two-hybrid Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular Cloning and Characterization of Phocein, a Protein Found from the Golgi Complex to Dendritic Spines

Baillat

Moqrich

Castets

et al. 2001

MBoC

115

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Distribution of Striatin, a newly identified calmodulin-binding protein in the rat brain: An in situ hybridization and immunocytochemical study

et al. 1998

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Striatin, a 110-kDa protein, is the first member of the tryptophane-aspartate repeat protein family known to bind calmodulin in the presence of Ca2+. We examined the distribution of striatin and its mRNA in the rat central nervous system (CNS) by using immunocytochemistry and in situ hybridization, respectively. Striatin immunostaining and mRNA labeling patterns are generally concordant. Regions showing the most intense staining are the dorsal striatum, nucleus accumbens (anterior and shell parts), olfactory tubercle, red nucleus, subthalamic nucleus, cranial nerve motor nuclei, and layer IX of the spinal cord (motoneurons). Low levels of both striatin and its mRNA are detected in the cerebral cortex, thalamus, septum, amygdala, hippocampus, midbrain and cerebellum. Striatin-immunoreactive neuronal processes are found predominantly in the structures containing striatin-positive neurons, suggesting that these labeled processes represent dendritic arborization rather than axonal processes. Except for the medial forebrain bundle, all axonal fiber tracts examined are devoid of striatin immunolabeling. These data show that the somatodendritic localization of striatin, previously described in the striatum, may be a main feature of the subcellular distribution of this protein throughout the CNS. Although widely distributed in neurons throughout the rat CNS, striatin is expressed prominently in the structures belonging to the motor system, suggesting that this protein may play a preponderant role in motor control.

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Distribution of Striatin, a newly identified calmodulin‐binding protein in the rat brain: An in situ hybridization and immunocytochemical study

Salin¹,

Kachidian²,

Bartoli³

et al. 1998

J. Comp. Neurol.

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A novel calmodulin-binding protein, belonging to the WD-repeat family, is localized in dendrites of a subset of CNS neurons.

Abstract: Abstract.A rat brain synaptosomal protein of 110,000

Cited by 118 publications

References 43 publications

Molecular Cloning and Characterization of Phocein, a Protein Found from the Golgi Complex to Dendritic Spines

Molecular Cloning and Characterization of Phocein, a Protein Found from the Golgi Complex to Dendritic Spines

Distribution of Striatin, a newly identified calmodulin-binding protein in the rat brain: An in situ hybridization and immunocytochemical study

Distribution of Striatin, a newly identified calmodulin‐binding protein in the rat brain: An in situ hybridization and immunocytochemical study

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