A novel C-type lectin (FcLec4) facilitates the clearance of Vibrio anguillarum in vivo in Chinese white shrimp

Wang, Xianwei; Zhang, Xiaowen; Xu, Wenteng; Zhao, Xiao‐Fan; Wang, Jinxing

doi:10.1016/j.dci.2009.05.004

Cited by 161 publications

(54 citation statements)

References 36 publications

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“…A growing number of CTLs have recently been discovered from marine invertebrates, especially crustaceans and molluscs, including those from F. chinensis [16,21,46,47], L. vannamei [15,19,45,48], Penaeus monodon [49,50], C. farreri [18,23,24,26], A. irradians [22,25] and Crassostrea gigas [51], Crassostrea virginica [52], and Haliotis discus discus [17]. Relatively little information is available on CTLs in echinoderms, and a handful of sea cucumber CTLs have been reported, such as MBL-C (Cucumaria japonica) [53], SJL-I (S. japonicus) [35,36], MBL-AJ and AJCTL (A. japonicus) [37,38].…”

Section: Discussionmentioning

confidence: 99%

“…For example, three CTLs from shrimp Litopenaeus vannamei (LvLectin-1, LvLectin-2 and LvCTL3) played a role in the immune response toward bacterial and viral infections [15,19]. Five CTLs from shrimp Fenneropenaeus chinensis agglutinated bacterial pathogens [16,20,21]. Additionally, several CTLs from the scallop Argopecten irradians and Chlamys farreri were involved in immune defense against invading microorganisms [22e26].…”

Section: Introductionmentioning

confidence: 99%

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Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Wei

Liu

Yang

et al. 2015

Fish & Shellfish Immunology

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a b s t r a c t C-type lectin is one important pattern recognition receptor (PRR) that plays crucial roles in multiple immune responses. A C-type lectin from sea cucumber Apostichopus japonicus (AjCTL-1) was characterized in the present study. The amino acid sequence of AjCTL-1 shared high similarities with other Ctype lectins from invertebrates and vertebrates. The C-type lectin domain (CTLD) of AjCTL-1 contained a Ca 2þ -binding site 2 and four conserved cysteine residues. AjCTL-1 mRNA expression patterns in tissues and after bacterial challenge were then analysed. Quantitative PCR revealed that AjCTL-1 mRNA was widely expressed in the tested tissues of healthy sea cucumber. The highest expression level occurred in gonad followed by body wall, coelomocytes, tentacle, intestinum and longitudinal muscle, and the lowest expression level was in respiratory tree. AjCTL-1 mRNA expression in coelomocytes was significantly induced by gram-negative Listonella anguillarum and gram-positive Micrococcus luteus, with different upregulation patterns post-challenge. Recombinant AjCTL-1 exhibited the ability to bind peptidoglycan directly, agglutinate M. luteus, Staphylococcus aureus and Escherichia coli, in a Ca 2þ -dependant manner, and enhance the phagocytosis of coelomocytes against E. coli in vitro. The results indicated that AjCTL-1 could act as a PRR in Apostichopus japonicus and had critical roles in non-self recognition and bacterial clearance against invading microbes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Wei

Liu

Yang

et al. 2015

Fish & Shellfish Immunology

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“…It was ubiquitously distributed in the gills, stomach, hepatopancreas, and intestine of Chinese white shrimp (26). We found that the FcLec4 antiserum could recognize a single band from the homogenates of the gills and stomach of kuruma shrimp (Fig.…”

Section: Fclec4 Homolog Existed In Kurumamentioning

confidence: 94%

“…7B) similar to the expression of full-length FcLec4 with the pET30a(ϩ) vector (Novagen) (26). The proteins were expressed as inclusion bodies, denatured in 8 M urea, and purified with Ni-NTA beads.…”

Section: Methodsmentioning

confidence: 99%

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C-type Lectin Binds to β-Integrin to Promote Hemocytic Phagocytosis in an Invertebrate

Wang¹,

Zhao²,

Wang³

2014

Journal of Biological Chemistry

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122

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Background: Our previous study found shrimp C-type lectin (FcLec4) facilitated bacterial clearance in vivo. Results: Native FcLec4 promoted hemocytic phagocytosis. The FcLec4 receptor was identified as ␤-integrin. Conclusion: FcLec4 functions as an opsonin, and ␤-integrin determines its opsonic activity. Significance: This is the first study to show the structural basis of an opsonin in crustaceans.

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C-type lectin from red swamp crayfish Procambarus clarkii participates in cellular immune response

Zhang

Wang

Sun

et al. 2011

Arch. Insect Biochem. Physiol.

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Lectins are potential immune recognition proteins. In this study, a novel C-type lectin (Pc-Lec1) is reported in freshwater crayfish Procambarus clarkii. Pc-Lec1 encodes a protein of 163 amino acids with a putative signal peptide and a single carbohydrate recognition domain. It was constitutively expressed in various tissues of a normal crayfish, especially in the hepatopancreas and gills. Expressions of Pc-Lec1 were up-regulated in the hepatopancreas and gills of crayfish challenged with Vibrio anguillarum, Staphylococcus aureus, or the white spot syndrome virus. Recombinant mature Pc-Lec1 bound bacteria and polysaccharides (peptidoglycan, lipoteichoic acid, and lipopolysaccharide) but did not agglutinate bacteria. Pc-Lec1 enhanced hemocyte encapsulation of the sepharose beads in vitro, and the blocking of beads by a polyclonal antibody inhibited encapsulation. Pc-Lec1 promoted clearance of V. anguillarum in vivo. These results suggest that Pc-Lec1 is a pattern recognition receptor and participates in cellular immune response. Pc-Lec1 performs its function as an opsonin by enhancing the encapsulation or clearance of pathogenic bacteria.

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A novel C-type lectin (FcLec4) facilitates the clearance of Vibrio anguillarum in vivo in Chinese white shrimp

Cited by 161 publications

References 36 publications

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

C-type Lectin Binds to β-Integrin to Promote Hemocytic Phagocytosis in an Invertebrate

C-type lectin from red swamp crayfish Procambarus clarkii participates in cellular immune response

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