A novel brainstem tumor model: functional and histopathological characterization

Jallo, George I.; Волков, А.А.; Wong, Cyrus; Carson, Benjamin S.; Penno, Margaret B.

doi:10.1007/s00381-006-0174-8

Cited by 37 publications

(33 citation statements)

References 25 publications

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“…4). 56,57,65,123 Wu et al 122 demonstrated that carboplatin could be locally delivered to neoplastic cells in a rat brainstem tumor model with mini-osmotic pumps, which resulted in a prolongation of survival. Moreover, Lonser et al 71 documented the safety of locally infused Gd-bound albumin via stereotactically implanted cannulas into large areas of the primate midbrain and pons, followed by other reports showing the safety of CED of chemotherapeutic agents, such as gemcitabine and carboplatin, infused into the primate brainstem, although dose-dependent toxicity has been found with carboplatin.…”

Section: New Therapeutic Perspective Strategiesmentioning

confidence: 99%

Treatment of diffuse intrinsic brainstem gliomas: failed approaches and future strategies

Frazier¹,

Lee²,

Thomale

et al. 2009

PED

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126

107

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Diffuse intrinsic pontine gliomas constitute ~ 60–75% of tumors found within the pediatric brainstem. These malignant lesions present with rapidly progressive symptoms such as cranial nerve, long tract, or cerebellar dysfunctions. Magnetic resonance imaging is usually sufficient to establish the diagnosis and obviates the need for surgical biopsy in most cases. The prognosis of the disease is dismal, and the median survival is < 12 months. Resection is not a viable option. Standard therapy involves radiotherapy, which produces transient neurological improvement with a progression-free survival benefit, but provides no improvement in overall survival. Clinical trials have been conducted to assess the efficacy of chemotherapeutic and biological agents in the treatment of diffuse pontine gliomas. In this review, the authors discuss recent studies in which systemic therapy was administered prior to, concomitantly with, or after radiotherapy. For future perspective, the discussion includes a rationale for stereotactic biopsies as well as possible therapeutic options of local chemotherapy in these lesions.

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Section: New Therapeutic Perspective Strategiesmentioning

confidence: 99%

Treatment of diffuse intrinsic brainstem gliomas: failed approaches and future strategies

Frazier¹,

Lee²,

Thomale

et al. 2009

PED

Self Cite

126

107

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“…There have been many laboratory studies investigating local delivery into an animal brainstem without tumors. Recently, a rat model of brainstem glioma has been developed [14,30]. Moreover, several feasibility studies were performed with continuous drug infusion into brainstem using carboplatin, BCNU, IL-13-PE, and gemcitabine [5,18,20,24].…”

Section: Introductionmentioning

confidence: 99%

“…We report our initial experience of temozolomide administration into the brainstem by intracerebral microinfusion using the guiding screw technique in the rat brainstem GBM allograft model established at our institution [11,13,14,16,30].…”

Section: Introductionmentioning

confidence: 99%

The effects of temozolomide delivered by prolonged intracerebral microinfusion against the rat brainstem GBM allograft model

et al. 2012

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“…Even if the automated infusion pump is cost prohibitive for some groups, cells can still be injected manually more quickly than stereotactic approaches. Overall, this rapid, accurate and reproducible method can be used to study glioma, medulloblastoma and brain stem tumors 7 . This procedure has been performed on over 500 mice between our two laboratories with deaths due to unexpected anesthetic reactions (<1%).…”

Section: Discussionmentioning

confidence: 99%

A Simple Guide Screw Method for Intracranial Xenograft Studies in Mice

Donoghue

Bögler

Johns

2011

JoVE

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The grafting of human tumor cells into the brain of immunosuppressed mice is an established method for the study of brain cancers including glioblastoma (glioma) and medulloblastoma. The widely used stereotactic approach only allows for the injection of a single animal at a time, is labor intensive and requires highly specialized equipment. The guide screw method, initially developed by Lal et al.,1 was developed to eliminate cumbersome stereotactic procedures. We now describe a modified guide screw approach that is rapid and exceptionally safe; both of which are critical ethical considerations. Notably, our procedure now incorporates an infusion pump that allows up to 10 animals to be simultaneously injected with tumor cells.To demonstrate the utility of this procedure, we established human U87MG glioma cells as intracranial xenografts in mice, which were then treated with AMG102; a fully human antibody directed to HGF/scatter factor currently undergoing clinical evaluation [2][3][4][5] . Systemic injection of AMG102 significantly prolonged the survival of all mice with intracranial U87MG xenografts and resulted in a number of complete cures.This study demonstrates that the guide screw method is an inexpensive, highly reproducible approach for establishing intracranial xenografts. Furthermore, it provides a relevant physiological model for validating novel therapeutic strategies for the treatment of brain cancers.

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A novel brainstem tumor model: functional and histopathological characterization

Abstract: The establishment of this animal tumor model will facilitate the testing of new therapeutic paradigms for the treatment of BSTs.

Cited by 37 publications

References 25 publications

Treatment of diffuse intrinsic brainstem gliomas: failed approaches and future strategies

Treatment of diffuse intrinsic brainstem gliomas: failed approaches and future strategies

The effects of temozolomide delivered by prolonged intracerebral microinfusion against the rat brainstem GBM allograft model

A Simple Guide Screw Method for Intracranial Xenograft Studies in Mice

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