A novel biosensor for Escherichia coli O157:H7 based on fluorescein-releasable biolabels

Hu, Rongrong; Yin, Zheng‐Zhi; Zeng, Yanbo; Zhang, Jian; Hai-qing, Liu; Shao, Yong; Ren, Shi‐Bin; Li, Lei

doi:10.1016/j.bios.2015.11.018

Cited by 60 publications

(22 citation statements)

References 22 publications

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“…Coli O157:H7 detection. Several techniques are available in laboratories for pathogenic bacteria detection and identification, including plating and culturing 2 , surface plasmon resonance 3–6 , electrochemistry 7–13 , quartz crystal microbalance 14–16 , fluorescent methods 17–22 . Each of these systems has its advantages, however, the utility of these methods is generally limited by their high cost and the requirement of trained operators.…”

Section: Introductionmentioning

confidence: 99%

Exploiting pH-Regulated Dimer-Tetramer Transformation of Concanavalin A to Develop Colorimetric Biosensing of Bacteria

Xu¹,

Yuan²,

Hu³

et al. 2017

Sci Rep

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Gold nanoparticles (AuNPs) aggregation-based colorimetric biosensing remains a challenge for bacteria due to their large size. Here we propose a novel colorimetric biosensor for rapid detection of Escherichia coli O157:H7 (E. coli O157:H7) in milk samples based on pH-regulated transformation of dimer/tetramer of Concanavalin A (Con A) and the Con A-glycosyl recognition. Briefly, antibody-modified magnetic nanoparticles was used to capture and concentrate E. coli O157:H7 and then to label with Con A; pH adjusted to 5 was then applied to dissociate Con A tetramer to release dimer, which was collected and re-formed tetramer at pH of 7 to cause the aggregation of dextran-modified AuNPs. The interesting pH-dependent conformation-transformation behavior of Con A innovated the design of the release from the bacteria surface and then the reconstruction of Con A. Therefore, we realized the sensitive colorimetric biosensing of bacteria, which are much larger than AuNPs that is generally not suitable for this kind of method. The proposed biosensor exhibited a limit of detection down to 41 CFU/mL, short assay time (~95 min) and satisfactory specificity. The biosensor also worked well for the detection in milk sample, and may provide a universal concept for the design of colorimetric biosensors for bacteria and virus.

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Section: Introductionmentioning

confidence: 99%

Exploiting pH-Regulated Dimer-Tetramer Transformation of Concanavalin A to Develop Colorimetric Biosensing of Bacteria

Xu¹,

Yuan²,

Hu³

et al. 2017

Sci Rep

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“…Rapid and sensitive methods that could timely diagnose this pathogen are the key to reduce the spread of infection and guarantee food safety at the source. The traditional microbiological culture method is considered as the standard method for bacterial detection ( Hu et al, 2016 ; Li et al, 2019 ). However, it is labor intensive and time consuming, usually needing more than 24 h of culture and analysis.…”

Section: Introductionmentioning

confidence: 99%

Rapid, Quantitative, High-Sensitive Detection of Escherichia coli O157:H7 by Gold-Shell Silica-Core Nanospheres-Based Surface-Enhanced Raman Scattering Lateral Flow Immunoassay

Shi

Xiao

et al. 2020

Front. Microbiol.

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Escherichia coli O157:H7 is regarded as one of the most harmful pathogenic microorganisms related to foodborne diseases. This paper proposes a rapiddetection biosensor for the sensitive and quantitative analysis of E. coli O157:H7 in biological samples by surface-enhanced Raman scattering (SERS)-based lateral flow immunoassay (LFIA). A novel gold-shell silica-core (SiO 2 /Au) nanosphere (NP) with monodispersity, good stability, and excellent SERS activity was utilized to prepare highperformance tags for the SERS-based LFIA system. The SiO 2 /Au SERS tags, which were modified with two layers of Raman reporter molecules and monoclonal antibodies, effectively bind with E. coli O157:H7 and form sandwich immune complexes on the test lines. E. coli O157:H7 was quantitatively detected easily by detecting the Raman intensity of the test lines. Under optimal conditions, the limit of detection (LOD) of the SiO 2 /Au-based SERS-LIFA strips for the target bacteria was 50 cells/mL in PBS solution, indicating these strips are 2,000 times more sensitive than colloidal Au-based LFIA strips. Moreover, the proposed assay demonstrated high applicability in E. coli O157:H7 detection in biological samples, including tap water, milk, human urine, lettuce extract and beef, with a low LOD of 100 cells/mL. Results indicate that the proposed SERS-based LFIA strip is applicable for the sensitive and quantitative determination of E. coli O157:H7.

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“…Biosensors [14] have been increasing in popularity as an alternative method due to their simplicity, speed, sensitivity, real-time monitoring, portability of the device and, potentially, low cost of testing [7,8]. Among numerous types of biosensors, the most frequently investigated appear to be those based on optical [15][16][17] and electrochemical [7,8] methods. Surface plasmon resonance (SPR)-based and electrochemical impedancebased sensors using gold (Au) surfaces have shown attractive sensitivity for bacterial detection [7,15], but have rarely been used to measure antibiotic sensitivity [18].…”

Section: Introductionmentioning

confidence: 99%

“…Photoluminescence-based biosensors have also been employed to determine antibiotic resistance of E. coli, but only with relatively low limits of detection [24]. The response of these biosensors depends on the efficiency of capturing target biomolecules with targetspecific ligands [26], such as antibodies [8,17], aptamers [27], antimicrobial peptides [28], lectins [15], phages [16] or molecular imprints [29] as opposed to non-specific binding to Au surface after coating with poly-lysine [18].…”

Section: Introductionmentioning

confidence: 99%

Binding strategies for capturing and growing Escherichia coli on surfaces of biosensing devices

Choinière

Frost

Dubowski

2019

Talanta

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Antibiotic resistant bacteria have become a threat to world health. An advanced method of detection, based on the matrix assisted laser desorption ionisation time-of-flight mass spectroscopy can identify bacteria relatively rapidly, but it is not suitable to measure bacterial antibiotic resistance. Biosensors may be able to detect resistance by monitoring growth after capture on sensor surfaces but this option has not been addressed adequately. We have evaluated the growth of Escherichia coli after capture in 96 well microplates and observed that growth/capture efficiency was relatively similar for antibody-based techniques, but non-specific capture varied considerably. We confirm that neutravidin binds E. coli non-specifically, which limited its use with biotinylated antibodies or aptamers. Centrifugation enhanced bacterial growth/capture considerably, indicating that procedures enhancing the interaction between bacteria and surface-bound antibody have the potential to improve growth efficiency. Capture and growth required larger numbers of bacteria than capture and detection on biosensor surfaces. Previously, we reported that the minimum concentration of live E. coli required for initiating growth on a GaAs/AlGaAs biosensor was ~ 10 5 CFU/mL [Nazemi et al., Talanta 178 (2018) 69-77], and we speculated that this could be related to the poisonous effect of Ga-and As-ions released during dark corrosion of the biosensor, however in the present report we observed that the same minimum concentration of E. coli was required for growth in an ELISA plate. Thus, we argue that this limitation was related rather to bacterial inhibition by the capture antibodies. Indeed, antibodies at titres designed to capture bacteria inhibited bacterial growth when the bacteria were added to growth medium at titres less than 10 5 CFU/mL, indicating that antibodies may be responsible for the higher limits of sensitivity due to their potential to restrict bacterial growth. However, we did not observe E. coli release after 6 h following the capture indicating that these bacteria did not degrade antibodies.

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A novel biosensor for Escherichia coli O157:H7 based on fluorescein-releasable biolabels

Cited by 60 publications

References 22 publications

Exploiting pH-Regulated Dimer-Tetramer Transformation of Concanavalin A to Develop Colorimetric Biosensing of Bacteria

Exploiting pH-Regulated Dimer-Tetramer Transformation of Concanavalin A to Develop Colorimetric Biosensing of Bacteria

Rapid, Quantitative, High-Sensitive Detection of Escherichia coli O157:H7 by Gold-Shell Silica-Core Nanospheres-Based Surface-Enhanced Raman Scattering Lateral Flow Immunoassay

Binding strategies for capturing and growing Escherichia coli on surfaces of biosensing devices

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