A novel biosensor based on multienzyme microcapsules constructed from covalent-organic framework

Liang, Huihui; Wang, Linyu; Yang, Yuxi; Song, Yonghai; Wang, Li

doi:10.1016/j.bios.2021.113553

Cited by 53 publications

(27 citation statements)

References 47 publications

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“…The reported linear range and LOD were 0.017-3 mM and 4.97 µM, respectively. Another example of multienzyme microcapsules constructed from a covalent-organic framework is reported by H. Liang et al [75] (Figure 5). In this work, the authors synthesized a core-shell structure enzyme@ZIF-8@COF by the Schiff base condensation reaction.…”

Section: Enzymatic Biosensorsmentioning

confidence: 92%

“…One of them involves the mixing of the COF with the conductive additives in a hollow glass tube [70,71]. A second strategy involves the electrode modification with Covalent Organic Nanosheets (also known as CONs [72,73]), produced by delamination of COFs usually by liquid phase exfoliation (LPE) and subsequent drop casting of CONs colloids [74][75][76]. Finally, the sensor's design can be based on simple electrodes [58,61] of dual systems, highlighting the sandwich-type electrodes [77,78].…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical (Bio)Sensors Based on Covalent Organic Frameworks (COFs)

Martínez‐Periñán

Martínez-Fernández

Segura

et al. 2022

Sensors

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Covalent organic frameworks (COFs) are defined as crystalline organic polymers with programmable topological architectures using properly predesigned building blocks precursors. Since the development of the first COF in 2005, many works are emerging using this kind of material for different applications, such as the development of electrochemical sensors and biosensors. COF shows superb characteristics, such as tuneable pore size and structure, permanent porosity, high surface area, thermal stability, and low density. Apart from these special properties, COF’s electrochemical behaviour can be modulated using electroactive building blocks. Furthermore, the great variety of functional groups that can be inserted in their structures makes them interesting materials to be conjugated with biological recognition elements, such as antibodies, enzymes, DNA probe, aptamer, etc. Moreover, the possibility of linking them with other special nanomaterials opens a wide range of possibilities to develop new electrochemical sensors and biosensors.

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Section: Enzymatic Biosensorsmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Electrochemical (Bio)Sensors Based on Covalent Organic Frameworks (COFs)

Martínez‐Periñán

Martínez-Fernández

Segura

et al. 2022

Sensors

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“…Taking this template-assisted encapsulation method, COFs microcapsules containing acetylcholinesterase (AChE), GOx and HRP were also built and used for electrochemical biosensors. [13] The obtained microcapsule features a capacious hollow cavity (ca. 600 nm), ensuring the conformational freedom of the encapsulated enzymes that favoring for the subsequent catalytic reaction.…”

Section: Cofsmentioning

confidence: 99%

Multienzyme Biocatalytic Cascade Systems in Porous Organic Frameworks for Biosensing

Gao

Zhong

Huang

et al. 2022

Chemistry A European J

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Multienzyme biocatalytic cascade systems (MBCS)have attracted widespread research in the field of biosensing due to selective substrate transformations and signal amplification function. However, the poor stability of enzymes significantly restricts their effectiveness in practical applications. The spatial organization of MBCS within porous organic frameworks (POFs), such as metal-organic frameworks, covalent organic frameworks, and hydrogen-bonded organic frameworks, is regarded as a promising strategy to overcome these challenges. This advanced biotechnology sets up a POFs microenvironment for enzymes immobilization, and thus make it possible to shield the enzyme from the external stimulus by POFs-guided structural confinement. Simultaneously, the tailorable porous structure of POFs shell allows for the selective transport of substrates into interior enzymes, thereby accelerating the sensing process. Herein, we present the concept of this POFs-confined MBCS, wherein enzymes were completely encapsulated into, rather than adsorption onto, the POFs. We highlight the new strategies for MBCS spatial organization through rational POFs support, and describe how this new bio-nanosystem that integrates framework and enzymes functions can be designed as a versatile biosensing platform. In addition, the challenges and outlooks are also discussed.

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“…Here, an electrochemical sensor based on the COF Thi-TFPB [ 48 , 49 , 50 , 51 ] loaded with AChE for the detection of carbaryl is proposed. The positively charged COF Thi-TFPB can be easily stripped into two-dimensional nanosheets, and modified on the surface of a bare glassy carbon electrode (GCE) without adhesives or conductive agents.…”

Section: Introductionmentioning

confidence: 99%

Biosensor Based on Covalent Organic Framework Immobilized Acetylcholinesterase for Ratiometric Detection of Carbaryl

Luo

Wang

et al. 2022

Biosensors

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A ratiometric electrochemical biosensor based on a covalent organic framework (COFThi-TFPB) loaded with acetylcholinesterase (AChE) was developed. First, an electroactive COFThi-TFPB with a two-dimensional sheet structure, positive charge and a pair of inert redox peaks was synthesized via a dehydration condensation reaction between positively charged thionine (Thi) and 1,3,5-triformylphenylbenzene (TFPB). The immobilization of AChE on the positively charged electrode surface was beneficial for maintaining its bioactivity and achieving the best catalytic effect; therefore, the positively charged COFThi-TFPB was an appropriate support material for AChE. Furthermore, the COFThi-TFPB provided a stable internal reference signal for the constructed AChE inhibition-based electrochemical biosensor to eliminate various effects which were unrelated to the detection of carbaryl. The sensor had a linear range of 2.2–60 μM with a detection limit of 0.22 μM, and exhibited satisfactory reproducibility, stability and anti-interference ability for the detection of carbaryl. This work offers a possibility for the application of COF-based materials in the detection of low-level pesticide residues.

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A novel biosensor based on multienzyme microcapsules constructed from covalent-organic framework

Cited by 53 publications

References 47 publications

Electrochemical (Bio)Sensors Based on Covalent Organic Frameworks (COFs)

Electrochemical (Bio)Sensors Based on Covalent Organic Frameworks (COFs)

Multienzyme Biocatalytic Cascade Systems in Porous Organic Frameworks for Biosensing

Biosensor Based on Covalent Organic Framework Immobilized Acetylcholinesterase for Ratiometric Detection of Carbaryl

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