A novel biosensor based on competitive SERS immunoassay and magnetic separation for accurate and sensitive detection of chloramphenicol

Kang, Yang; Hu, Yongjun; Dong, Ning

doi:10.1016/j.bios.2016.01.064

Cited by 110 publications

(39 citation statements)

References 48 publications

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“…To use SERS to detect binding events, we conjugate one affinity reagent to gold nanoparticles (AuNPs) and the other to a gold metal film. The AuNP-Au film sandwich structure formed in the presence of the target molecule becomes a SERS hot spot, generating a strong and unambiguous signal that indicates a true positive (10). One can analyze the SERS signals by using the traditional "analog" method to correlate the intensity of one Raman reporter to the known protein concentration, thereby developing an intensity vs. concentration calibration curve (5,6,10,11).…”

mentioning

confidence: 99%

“…The AuNP-Au film sandwich structure formed in the presence of the target molecule becomes a SERS hot spot, generating a strong and unambiguous signal that indicates a true positive (10). One can analyze the SERS signals by using the traditional "analog" method to correlate the intensity of one Raman reporter to the known protein concentration, thereby developing an intensity vs. concentration calibration curve (5,6,10,11). In contrast, we have opted for an improved "digital" method, in which many SERS spectra are collected in a predetermined area of the gold surface under computer control, after which true positives can be identified by using a classical least-squares (CLS) approach to measure the number of sites that report significant contributions from both reporters.…”

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confidence: 99%

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Dual-reporter SERS-based biomolecular assay with reduced false-positive signals

Chuong

Pallaoro

Chaves

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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We present a sensitive and quantitative protein detection assay that can efficiently distinguish between specific and nonspecific target binding. Our technique combines dual affinity reagents with surfaceenhanced Raman spectroscopy (SERS) and chemometric analysis. We link one Raman reporter-tagged affinity reagent to gold nanoparticles and another to a gold film, such that protein-binding events create a "hot spot" with strong SERS spectra from both Raman reporter molecules. Any signal generated in this context is indicative of recognition by both affinity labels, whereas signals generated by nonspecific binding lack one or the other label, enabling us to efficiently distinguish true from false positives. We show that the number of hot spots per unit area of our substrate offers a quantitative measure of analyte concentration and demonstrate that this duallabel, SERS-linked aptasensor assay can sensitively and selectively detect human α-thrombin in 1% human serum with a limit of detection of 86 pM.surface-enhanced Raman spectroscopy | SERS | protein detection | nanoparticle | ELISA

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confidence: 99%

mentioning

confidence: 99%

Dual-reporter SERS-based biomolecular assay with reduced false-positive signals

Chuong

Pallaoro

Chaves

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…Functionalized magnetic beads are widely used in LFA (Hwang et al, 2016;Tang et al, 2009), and LFAs based on magnetic separation provide several advantages, such as easy separation, short analytical times, and wide linear ranges (Yang, Hu, & Dong, 2016). In Liu's work (Liu et al, 2015), a two-step LFA based on immunomagnetic beads was developed to detect trace Aflatoxin M 1 in milk.…”

Section: Introductionmentioning

confidence: 99%

Dual signal insight: field-efficient qualitative/quantitative detection of sulphamethazine in raw milk

Song

Huang

Chen

et al. 2019

Food and Agricultural Immunology

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A novel lateral flow assay (LFA) was developed by introducing magnetic beads with colloidal gold nanoparticles (AuMB) to LFA test strips for the qualitative and quantitative detection of sulphamethazine (SM 2 ) in raw milk. In the proposed assay, test samples are qualitatively judged yes (positive) or no (negative) on the basis of naked-eye signals from the colloidal gold and fluorescence quenching is used to quantitatively determine the level of SM 2 in positive samples. Results indicated that the threshold concentration for determining whether a test sample is positive or negative is 5 ng/mL. The limit of detection and linear range of the proposed AuMB-LFA for quantitative detection were 0.39 and 0.5-200 ng/mL, respectively, and its recovery for detecting SM 2 in raw milk was 97.4-110.2%. The developed AuMB-LFA provides novel insights into efforts to maintain a balance between the efficient screening of targets in large samples and saving time and money. ARTICLE HISTORY

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“…Magnetic properties have been widely utilized as a component of complex nanostructures in multifunctional SERS active NPs [18,19,20,21]. They are usually exploited for the separation of cells [22] or small molecules [23] because NP movement is easily controlled by applying an external magnetic field.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Magnetic-SERS Dual-Function Silica Nanoprobes for Effective On-Site Organic Chemical Detection

et al. 2017

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We report magnetic silver nanoshells (M-AgNSs) that have both magnetic and SERS properties for SERS-based detection. The M-AgNSs are composed of hundreds of Fe3O4 nanoparticles for rapid accumulation and bumpy silver shell for sensitive SERS detection by near-infrared laser excitation. The intensity of the SERS signal from the M-AgNSs was strong enough to provide single particle-level detection. We obtained much stronger SERS signal intensity from the aggregated M-AgNSs than from the non-aggregated AgNSs. 4-Fluorothiophenol was detected at concentrations as low as 1 nM, which corresponds to 0.16 ppb. The limit of detection for tetramethylthiuram disulfide was 10 μM, which corresponds to 3 ppm. The M-AgNSs can be used to detect trace amounts of organic molecules using a portable Raman system.

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A novel biosensor based on competitive SERS immunoassay and magnetic separation for accurate and sensitive detection of chloramphenicol

Cited by 110 publications

References 48 publications

Dual-reporter SERS-based biomolecular assay with reduced false-positive signals

Dual-reporter SERS-based biomolecular assay with reduced false-positive signals

Dual signal insight: field-efficient qualitative/quantitative detection of sulphamethazine in raw milk

Highly Sensitive Magnetic-SERS Dual-Function Silica Nanoprobes for Effective On-Site Organic Chemical Detection

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