A Novel Biosensor Based on Glucose Oxidase for Activity Determination of α – Amylase

Abstract

A glucose oxidase-based biosensor was developed for the determination of α-amylase activity. The determination method is based on monitoring the decrease in dissolved oxygen concentration related to the starch concentration, for which starch gives a reaction with α-amylase. Optimization parameters, including glucose oxidase amount, gelatin amount, and glutaraldehyde percentage for cross-linking, were investigated. The effects of pH, buffer system, and temperature on the biosensor system were also investigated. The biosensor had a linear relation to α-amylase activity and good measurement correlation between 0.66 and 9.83 U/ml. In sample analysis studies, α-amylase activity in baker's yeast was determined by the biosensor.

Enzyme functionalized microgels enable precise regulation of dissolved oxygen and anaerobe culture

“…because it is both simple and scalable. We dispersed a mixture of 10% w/ w gelatin, 904 U mL À1 GOx (with or without 904 U mL À1 CAT), and 1 mM glutaraldehyde in deionized water into mineral oil containing 1.0% v/v Span 80 [32]. We selected gelatin as a support matrix to immobilize GOx because of its low cost, ease of handling, biocompatibility, and tunability as a hydrogel support matrix [33][34][35].…”

Çevrimsel Voltametri Tabanlı Mikroakışkan Sistem Tasarımı ve Analizi

“…Keywords: Glucose determination, Cyclic voltammetry, Biosensor, Finite element analysis [3]. Sisteme biyosensör eklenmesiyle klasik elektrokimya mantığındaki anyon ve katyon ölçümü dışında birçok madde tayini yapmak biyosensörlerin sistemlerdeki en önemli avantajlarındandır [4].…”