A novel biomimetic immunoassay method based on Pt nanozyme and molecularly imprinted polymer for the detection of histamine in foods

Abstract

This study used surface imprinting polymerization to synthesize molecularly imprinted polymer on the surface of PAF-45 material (PAF-45@MIP). SEM, FT-IR, static and kinetic adsorption experiments were adopted to investigate the morphology and properties of the MIP. The results showed that the PAF-45@MIP could rapidly absorb histamine with a high adsorption capacity. Using PAF-45@MIP as a biomimetic antibody and Pt nanozyme as a marker, a novel direct competitive biomimetic immunoassay (BIA) method for rapidly detecting histamine in foods was developed. Under optimal conditions, the limit of detection and sensitivity of the BIA method were 0.128 and 8.306 mg/L, respectively. Vinegar spiked with histamine was determined using the BIA method with recoveries ranging from 81.50% to 90.38%. The BIA method was also applied to determine histamine in dried shrimp and soy sauce samples, the results correlating well with those obtained using high-performance liquid chromatography.

Molecular Imprinting on Nanozymes for Sensing Applications

“…This ideal combination was further improved by preparing MIP films for selective recognition and reusability, allowing to develop assays whose results were comparable to those obtained by HPLC [ 94 , 95 ]. Other configurations have been proposed, such as the use of Pt@SiO 2 NPs and a MIP for highly sensitive and selective detection of histamine with a limit of detection of 0.128 mg L −1 [ 96 ].…”

A sensitive biomimetic enzyme-linked immunoassay method based on Au@Pt@Au composite nanozyme label and molecularly imprinted biomimetic antibody for histamine detection

“…In recent years, many new ELISA technologies based on biomimetic antibody and nanozyme have been reported (He et al, 2020;Wang et al, 2020). Molecularly imprinted polymers (MIPs) are man-made functionalized materials, which have specific recognition ability toward the template molecules (Teixeira et al, 2021;Trevizan et al, 2021).…”

“…Compared with biology antibodies, MIPs have many advantages to be used as artificial antibody in the ELISA method due to their excellent properties of high selectivity, simple fabrication, good stability and reusability (Lata et al, 2015;Li et al, 2017;Wulff, 1995;Ye & Mosbach, 2008;Yu et al, 2020). Nanozymes are functional nanomaterials with enzyme-like characteristics, which can be used in ELISA because of their adjustable enzyme-like catalytic activity, simple preparation, low cost and high stability (He et al, 2020;Huang et al, 2019;Wang et al, 2020;Wu et al, 2019Wu et al, , 2020Zhou et al, 2017). Ataee-Esfahani and co-workers suggested that the Au@Pt nanomaterial could act as a potential peroxidase mimetics, which can oxidize substrate tetramethylbenzidine (Ataee-Esfahani et al, 2010).…”