A novel biomarker explaining the role of oxidative stress in exercise andl-tyrosine supplementation: thiol/disulphide homeostasis

Abstract

The present study aimed to evaluate the relationship between exercise and both l-tyrosine and oxidative stress using thiol/disulphide homeostasis via a novel biomarker in rats. Following the completion of the exercise and l-tyrosine protocol, serum total thiol, native thiol, and disulphide concentrations were determined using a novel automated measurement method. Compared with the control group, serum dynamic disulphide levels were significantly lower in the E group (116.75 ± 10.49; p < .05) and the highest in the LT group (151.0 ± 5.84). The lowest oxidised thiol (49.75 ± 6.18; p = .087) and the highest reduced thiol (75.38 ± 3.16; p = .079) rates were determined to be in the E group. The highest oxidised thiol value was observed in the LT group. Exercise positively affects thiol/disulphide homeostasis, which is a novel indicator of oxidant-antioxidant parameters. Additionally, l-tyrosine appears to be more convenient combined with exercise. The new method used in our study proposes a promising, practical, and useful method for assessing the oxidative stress parameters.

The effects of moderate running exercise and L-tyrosine on penicillin-induced epileptiform activity in rats

“…Epileptic seizures are a common feature of mitochondrial dysfunction associated with mitochondrial encephalopathies (Patel, 2004). However, physical exercise has been reported to be effective in the treatment of oxidative stress and impaired brain function that is associated with oxidative stress (Kim et al, 2013;Kayacan et al, 2017). Accordingly, the mid-level running exercise that was performed in the present study reduced epileptiform activity, which may be caused by oxidative stress.…”

“…The antioxidant enzyme activity is significantly increased in athletes and exercised rats. The increased antioxidant activity then inhibits lipid peroxidation caused by an exercise-induced increase in oxidative stress (Kayacan et al, 2017;2019). Additionally, individuals exercising regularly have an advantage over sedentary people because training supports the activity of many major antioxidant enzymes and development of the overall antioxidant level.…”

“…L-tyrosine is an antioxidant/amino acid that has amphiphilic properties because of its polar and apolar group characteristics (Gemili et al, 2010) and its antioxidant activity results from its side chain that can donate protons to free radicals. This property is similar to aromatic amino acids such as phenylalanine and tryptophan (Kayacan et al, 2017). Research has shown that a tyrosine supplement or food that is nutritionally rich in tyrosine increases dopamine (DA) and norepinephrine release in the brain (Robertson et al, 2016).…”

An Analysis of Plasma Thiol/Disulphide Homeostasis in Patients with Stable Angina

“…18 This novel indicator of oxidative stress was examined in children with simple febrile seizure and the result has shown that the seizure may cause disruption in favor of disulfide bonds. 19 In a previous study, Kayacan et al 20 aimed to evaluate the relationship between exercise and both l-tyrosine and oxidative stress using this new method and the result showed that exercise positively affected thiol/disulphide homeostasis. Moreover, this prognostic biomarker was applied in non small cell lung cancer patient and total thiol, native thiol and disulphide levels was found decreased in patients.…”

Thiol-Disulphide Homoeostasis in COVID-19: Evaluation of its Relationship with Complete Blood Count Parameters

“…Thiols are most important defense system against reactive species due to sulfhydryl groups (SH) in their structure. SH groups can be oxidized by the oxidant molecules in the environment and converted into reversible disulfide (SS) bond structures (11). The disulfide bond structures formed in this way are reduced back to thiol (SH) groups, and the thiol-disulfide balance is preserved (12).…”