A Novel Biodegradable Composite Polymer Material Based on PLGA and Silver Oxide Nanoparticles with Unique Physicochemical Properties and Biocompatibility with Mammalian Cells

Abstract

A method for obtaining a stable colloidal solution of silver oxide nanoparticles has been developed using laser ablation. The method allows one to obtain nanoparticles with a monomodal size distribution and a concentration of more than 108 nanoparticles per mL. On the basis of the obtained nanoparticles and the PLGA polymer, a nanocomposite material was manufactured. The manufacturing technology allows one to obtain a nanocomposite material without significant defects. Nanoparticles are not evenly distributed in the material and form domains in the composite. Reactive oxygen species (hydrogen peroxide and hydroxyl radical) are intensively generated on the surfaces of the nanocomposite. Additionally, on the surface of the composite material, an intensive formation of protein long-lived active forms is observed. The ELISA method was used to demonstrate the generation of 8-oxoguanine in DNA on the developed nanocomposite material. It was found that the multiplication of microorganisms on the developed nanocomposite material is significantly decreased. At the same time, the nanocomposite does not inhibit proliferation of mammalian cells. The developed nanocomposite material can be used as an affordable and non-toxic nanomaterial to create bacteriostatic coatings that are safe for humans.

Ag2O Nanoparticles as a Candidate for Antimicrobial Compounds of the New Generation

“…To prevent the premature aggregation of synthesized Ag 2 O NPs, a surfactant-for example, citrate, polyethylene glycol, triethylene glycol, chitosan, urea and other compounds-can be added to the reaction mixture [40,82,[96][97][98][99]. Another method for Ag 2 O NP production is the reduction of AgNO 3 using organic acids citrate, acetate and oleic acid [45,53,56]. In the literature, this method is sometimes called the sol-gel method [100].…”

PLGA-Based Nanomedicine: History of Advancement and Development in Clinical Applications of Multiple Diseases

“…Therefore, GS loaded PLGA-GKK encapsulated PNPs were elicited for the treatment of SSI at the localized site [133]. Additionally, synthesis of the PLGA/Ag2O NPs composite was performed by a low-temperature technique established by Smirnova et al [134]. The nanocomposite produced encouraging findings, suggesting it may be used to create materials with both potent antibacterial activity and great biocompatibility with human cells.…”

“…The nanocomposite produced encouraging findings, suggesting it may be used to create materials with both potent antibacterial activity and great biocompatibility with human cells. Such materials have potential uses in surgical procedures, especially in prosthetics [134].…”

Synthesis of a Novel, Biocompatible and Bacteriostatic Borosiloxane Composition with Silver Oxide Nanoparticles

“…Currently, there is a significant growth of interest in metal oxide nanoparticles (Me x O y NPs) as compounds with antibacterial potential, including Ag [1,2], which find many applications in technology and medicine. Despite the large number of antibiotics available for treatment, resistance to almost all of them has been confirmed.…”