A novel bioconversion of l-fructose to l-glucose by Klebsiella pneumoniae

Leang, Khim; Sultana, Ishrat; Takada, Goro; Izumori, Ken

doi:10.1016/s1389-1723(03)80036-1

Cited by 22 publications

(8 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ketoses 7 – 12 have important biological functions. d -tagatose 7 (C 6 ) displays antidiabetic properties and is used in the food industry as a hypocaloric sweetener. , l -psicose 8 (C 6 ) is used for the synthesis of l -fructose, precursor of an inhibitor of glucosidases. , d - altro -heptulose ( d -sedoheptulose) 9 (C 7 ) is a marker of sugar metabolism disorders such as cystinose. , d - ido -heptulose 10 has been reported to be a precursor of valiolamine and N -substituted derivatives, glucosidase inhibitors useful for the treatment of hyperglycemic symptoms and its (5 S )-epimer d - glycero- d - ido- octulose 12 , which is very abundant in Craterostigma plantagineum , has been described as a plant antioxidant involved in desiccation tolerance and could be a potential reactive oxygen species (ROS) scavenger with applications in nutrition and healthcare …”

Section: Introductionmentioning

confidence: 99%

Evolved Thermostable Transketolase for Stereoselective Two-Carbon Elongation of Non-Phosphorylated Aldoses to Naturally Rare Ketoses

et al. 2019

View full text Add to dashboard Cite

We propose an ecofriendly, efficient, stereoselective procedure for the two-carbon elongation of nonphosphorylated aldoses (C4–C6) to the corresponding C n+2 ketoses (C6–C8) in one step, using hydroxypyruvate (HPA) as a ketol donor substrate and an evolved thermostable transketolase from Geobacillus stearothermophilus (TKgst) as a biocatalyst. Simultaneous site saturation mutagenesis (SSM) at two or three key positions in the TKgst active site yielded efficient variants, L382F/F435Y, R521Y/S385/H462N, and R521V/S385D/H462S, with increased activity compared to wild-type TKgst for conversion of two tetroses (d-threose, l-erythrose), two pentoses (d-xylose, d-ribose), and two hexoses (d-allose, d-glucose), respectively. These six C n aldoses as acceptor and HPA as donor substrates were transformed by the TKgst variants at 60 °C with practically complete conversion. The corresponding C n+2 ketoses, including two hexuloses (d-tagatose, l-psicose), two heptuloses (d-altro-heptulose, d-ido-heptulose), and two octuloses (d-glycero- d-ido-octulose, d-glycero-d-altro-octulose) are naturally rare compounds with important biological functions, which were obtained with high diastereoselectivity.

show abstract

Section: Introductionmentioning

confidence: 99%

Evolved Thermostable Transketolase for Stereoselective Two-Carbon Elongation of Non-Phosphorylated Aldoses to Naturally Rare Ketoses

et al. 2019

View full text Add to dashboard Cite

show abstract

“…L-Glucose can also be used as an ideal starting material for glycoconjugate vaccines against the enteropathogenic bacterium Shigella sonne [80]. Enzymatically, L-glucose can be synthesized by isomerizing L-fructose (Scheme 13) catalyzed by D-xylose isomerase from Candida utilis [81] or whole cells of a mutant Klebsiella pneumoniae strain, which constitutively express D-arabinose isomerase (EC 5.3.1.3) [82]. An overall yield of 35% on a gram-scale was reported using this mutant strain [82].…”

Section: Reviewmentioning

confidence: 99%

“…Enzymatically, L-glucose can be synthesized by isomerizing L-fructose (Scheme 13) catalyzed by D-xylose isomerase from Candida utilis [81] or whole cells of a mutant Klebsiella pneumoniae strain, which constitutively express D-arabinose isomerase (EC 5.3.1.3) [82]. An overall yield of 35% on a gram-scale was reported using this mutant strain [82]. Moreover, Yadav et al immobilized galactose oxidase (EC 1.1.3.9) and catalase (EC 1.11.1.6) on a solid support (crab-shell particles or Ocimum sanctum seeds) and investigated their synthetic application for L-glucose production from D-sorbitol (Scheme 13) [83–84].…”

Section: Reviewmentioning

confidence: 99%

Biosynthesis of rare hexoses using microorganisms and related enzymes

Li¹,

Gao²,

Nakanishi³

et al. 2013

Beilstein J. Org. Chem.

View full text Add to dashboard Cite

SummaryRare sugars, referred to as monosaccharides and their derivatives that rarely exist in nature, can be applied in many areas ranging from foodstuffs to pharmaceutical and nutrition industry, or as starting materials for various natural products and drug candidates. Unfortunately, an important factor restricting the utilization of rare sugars is their limited availability, resulting from limited synthetic methods. Nowadays, microbial and enzymatic transformations have become a very powerful tool in this field. This article reviews the biosynthesis and enzymatic production of rare ketohexoses, aldohexoses and sugar alcohols (hexitols), including D-tagatose, D-psicose, D-sorbose, L-tagatose, L-fructose, 1-deoxy-L-fructose, D-allose, L-glucose, L-talose, D-gulose, L-galactose, L-fucose, allitol, D-talitol, and L-sorbitol. New systems and robust catalysts resulting from advancements in genomics and bioengineering are also discussed.

show abstract

“…Furthermore, ethanol is produced by the fermentation of l -xylulose using yeast ( Chiang et al, 1981b ). l -Xylulose has several applications, including its use as a potential inhibitor of various glucosidases ( Leang et al, 2003 ) and in medicine. For example, it is a reliable indicator of acute or chronic hepatitis ( Granström et al, 2005 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a xylitol dehydrogenase from Aspergillus flavus and its application in l-xylulose production

Kumar

Li²,

Kondaveeti³

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

An NAD+-dependent xylitol dehydrogenase from A. flavus (AfXDH) was cloned and successfully expressed in Escherichia coli. AfXDH gene sequence revealed an open reading frame of 1,110 bp, encoding a polypeptide of 369 amino acids with a calculated molecular mass of 38,893 Da. Among various polyols, sorbitol and xylitol were preferred substrates of AfXDH with Km values of 16.2 and 16.9 mM, respectively. AfXDH showed the highest activity in Tris-glycine-NaOH buffer (pH 9.5) at 50°C; it required Zn2+ or Mn2+ for enzyme activity. The half-life at 40°C and half denaturation temperature (T1/2) was 200 min and 45°C, respectively. Bioinformatic analyses along with biochemical properties confirmed that AfXDH belonged to the medium-chain dehydrogenase/reductase family. AfXDH exhibits higher thermostability and kcat values than those of other XDHs. The feasibility of using AfXDH in l-xylulose production was demonstrated. AfXDH, when coupled with Streptococcus pyogenes NADH oxidase, efficiently converted xylitol to l-xylulose with 97% yield, suggesting its usefulness for the industrial l-xylulose production from xylitol.

show abstract

A novel bioconversion of l-fructose to l-glucose by Klebsiella pneumoniae

Cited by 22 publications

References 16 publications

Evolved Thermostable Transketolase for Stereoselective Two-Carbon Elongation of Non-Phosphorylated Aldoses to Naturally Rare Ketoses

Evolved Thermostable Transketolase for Stereoselective Two-Carbon Elongation of Non-Phosphorylated Aldoses to Naturally Rare Ketoses

Biosynthesis of rare hexoses using microorganisms and related enzymes

Characterization of a xylitol dehydrogenase from Aspergillus flavus and its application in l-xylulose production

Contact Info

Product

Resources

About