A novel biocompatible chitosan–Selenium nanoparticles (SeNPs) film with electrical conductivity for cardiac tissue engineering application

Kalishwaralal, Kalimuthu; Jeyabharathi, Subhaschandrabose; Sundar, Krishnan; Sethuraman, S; Prasanna, M.; Muthukumaran, Azhaguchamy

doi:10.1016/j.msec.2018.06.036

Cited by 88 publications

(45 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Selenium NPs, obtained by a novel one-pot green synthesis, were used to modify chitosan film; a recent study confirmed the potential of substrate modified with these nanoparticles to provide electrical conductivity (0.0055 S cm −1 ) in order to promote rapid electrical coupling between CMs, and enhance mechanical properties in agreement with those of native myocardium [48].…”

Section: Inorganic Nanoparticlesmentioning

confidence: 92%

Nanoengineering in Cardiac Regeneration: Looking Back and Going Forward

et al. 2020

View full text Add to dashboard Cite

To deliver on the promise of cardiac regeneration, an integration process between an emerging field, nanomedicine, and a more consolidated one, tissue engineering, has begun. Our work aims at summarizing some of the most relevant prevailing cases of nanotechnological approaches applied to tissue engineering with a specific interest in cardiac regenerative medicine, as well as delineating some of the most compelling forthcoming orientations. Specifically, this review starts with a brief statement on the relevant clinical need, and then debates how nanotechnology can be combined with tissue engineering in the scope of mimicking a complex tissue like the myocardium and its natural extracellular matrix (ECM). The interaction of relevant stem, precursor, and differentiated cardiac cells with nanoengineered scaffolds is thoroughly presented. Another correspondingly relevant area of experimental study enclosing both nanotechnology and cardiac regeneration, e.g., nanoparticle applications in cardiac tissue engineering, is also discussed.

show abstract

Section: Inorganic Nanoparticlesmentioning

confidence: 92%

Nanoengineering in Cardiac Regeneration: Looking Back and Going Forward

et al. 2020

View full text Add to dashboard Cite

show abstract

“…86 Despite the effective applications of Au NPs in cardiac tissue engineering, one of their disadvantages is their toxicity.- 87 Selenium NPs (SeNPs) are among those naturally produced in the body, and have been used for its therapeutic potential for cardiovascular disease, because diseased individuals have very low levels of this element in blood. 88,89 In 2018, Kalishwaralal et al synthesized a chitosan-selenium NP (SeNP) nanocomposite film with high electrical conductivity (0.0055S cm −1 ) and a suitable tensile strength of 419 kPa which matched properties of the native myocardium. Evaluation of this film by a H9C2 cell line showed that cells grew on the chitosan-SeNPs films, were capable of proliferation and also formed membrane nanotubes, indicating the transmission of electrical signals between cells and the conductive matrices and the communication between cells, causing more cardiac cell activity, cardiac repair and increases in electrical conductivity more than the natural myocardium of the heart.…”

Section: Dovepressmentioning

confidence: 99%

“…By utilizing different methods in the synthesis of these polymeric nano fibers, or even in the sterilizing process, the desired morphoology can be obtained. Figure 8 shows the difference between the morphologies of a typical nano fiber preparation by using different sterilizing methods 88 (Figure 8).…”

Section: Dovepressmentioning

confidence: 99%

<p>Biodegradable Nanopolymers in Cardiac Tissue Engineering: From Concept Towards Nanomedicine</p>

Nasr¹,

Rabiee²,

Hajebi³

et al. 2020

IJN

View full text Add to dashboard Cite

Cardiovascular diseases are the number one cause of heart failure and death in the world, and the transplantation of the heart is an effective and viable choice for treatment despite presenting many disadvantages (most notably, transplant heart availability). To overcome this problem, cardiac tissue engineering is considered a promising approach by using implantable artificial blood vessels, injectable gels, and cardiac patches (to name a few) made from biodegradable polymers. Biodegradable polymers are classified into two main categories: natural and synthetic polymers. Natural biodegradable polymers have some distinct advantages such as biodegradability, abundant availability, and renewability but have some significant drawbacks such as rapid degradation, insufficient electrical conductivity, immunological reaction, and poor mechanical properties for cardiac tissue engineering. Synthetic biodegradable polymers have some advantages such as strong mechanical properties, controlled structure, great processing flexibility, and usually no immunological concerns; however, they have some drawbacks such as a lack of cell attachment and possible low biocompatibility. Some applications have combined the best of both and exciting new natural/ synthetic composites have been utilized. Recently, the use of nanostructured polymers and polymer nanocomposites has revolutionized the field of cardiac tissue engineering due to their enhanced mechanical, electrical, and surface properties promoting tissue growth. In this review, recent research on the use of biodegradable natural/synthetic nanocomposite polymers in cardiac tissue engineering is presented with forward looking thoughts provided for what is needed for the field to mature.

show abstract

“…Since primary cardiomyocytes do not proliferate, we have chosen H9c2 cells as an alternative to primary cardiomyocytes; these cells maintain morphological characteristics of immature embryonic cardiomyocytes with electrical and hormonal signal pathway elements of adult cardiac cells. These cells have been extensively used in cardiovascular research and specifically in other electric stimulation assays [30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Bioelectronics-on-a-chip for cardio myoblast proliferation enhancement using electric field stimulation

et al. 2020

View full text Add to dashboard Cite

Background Cardio myoblast generation from conventional approaches is laborious and time-consuming. We present a bioelectronics on-a-chip for stimulating cells cardio myoblast proliferation during culture. Method The bioelectronics chip fabrication methodology involves two different process. In the first step, an aluminum layer of 200 nm is deposited over a soda-lime glass substrate using physical vapor deposition and selectively removed using a Q-switched Nd:YVO4 laser to create the electric tracks. To perform the experiments, we developed a biochip composed of a cell culture chamber fabricated with polydimethylsiloxane (PDMS) with a glass coverslip or a cell culture dish placed over the electric circuit tracks. By using such a glass cover slip or cell culture dish we avoid any toxic reactions caused by electrodes in the culture or may be degraded by electrochemical reactions with the cell medium, which is crucial to determine the effective cell-device coupling. Results The chip was used to study the effect of electric field stimulation of Rat ventricular cardiomyoblasts cells (H9c2). Results shows a remarkable increase in the number of H9c2 cells for the stimulated samples, where after 72 h the cell density double the cell density of control samples. Conclusions Cell proliferation of Rat ventricular cardiomyoblasts cells (H9c2) using the bioelectronics-on-a-chip was enhanced upon the electrical stimulation. The dependence on the geometrical characteristics of the electric circuit on the peak value and homogeneity of the electric field generated are analyzed and proper parameters to ensure a homogeneous electric field at the cell culture chamber are obtained. It can also be observed a high dependence of the electric field on the geometry of the electrostimulator circuit tracks and envisage the potential applications on electrophysiology studies, monitoring and modulate cellular behavior through the application of electric fields.

show abstract

A novel biocompatible chitosan–Selenium nanoparticles (SeNPs) film with electrical conductivity for cardiac tissue engineering application

Cited by 88 publications

References 25 publications

Nanoengineering in Cardiac Regeneration: Looking Back and Going Forward

Nanoengineering in Cardiac Regeneration: Looking Back and Going Forward

<p>Biodegradable Nanopolymers in Cardiac Tissue Engineering: From Concept Towards Nanomedicine</p>

Bioelectronics-on-a-chip for cardio myoblast proliferation enhancement using electric field stimulation

Contact Info

Product

Resources

About