A novel biliary stent coated with silver nanoparticles prolongs the unobstructed period and survival via anti-bacterial activity

Abstract

Symptomatic biliary stricture causes life-threatening complications, such as jaundice, recurrent cholangitis and secondary biliary cirrhosis. Fully covered self-expanding metal stents (FCSEMSs) are gaining acceptance for treatments of benign biliary stricture and palliative management of malignant biliary obstructions. However, the high rate of FCSEMS obstruction limits their clinic use. In this study, we developed a novel biliary stent coated with silver nanoparticles (AgNPs) and investigated its efficacy both in vitro and in vivo. We first identified properties of the AgNP complex using ultraviolet detection. The AgNP complex was stable without AgNP agglomeration, and Ag abundance was correspondingly increased with an increased bilayer number. The AgNP biliary stent demonstrated good performance in the spin-assembly method based on topographic observation. The AgNP biliary stent also exhibited a long-term anti-coagulation effect and a slow process of Ag+ release. In vitro anti-bacteria experiments indicated that the AgNP biliary stent exhibited high-efficiency anti-bacterial activity for both short- and long-term periods. Importantly, application of the AgNP biliary stent significantly prolonged the unobstructed period of the biliary system and improved survival in preclinical studies as a result of its anti-microbial activity and decreased granular tissue formation on the surface of the anastomotic biliary, providing a novel and effective treatment strategy for symptomatic biliary strictures.

Fabrication of a silver particle-integrated silicone polymer-covered metal stent against sludge and biofilm formation and stent-induced tissue inflammation

“…An in vivo animal study revealed that coating of a polyurethane stent with silver nanoparticles inhibited bacterial adhesion for 7 days 16 . In another study, a silver-coated biliary plastic stent prolonged the unobstructed period, owing to its antibacterial activity 17 . These results suggested that coating of plastic stents with silver nanoparticles resulted in the release of nano-silver, which inhibited bacterial growth and prolonged stent patency.…”

“…To prevent biofilm formation on biliary stents, several in vitro and in vivo animal studies have reported the bactericidal efficacy of silver-coated plastic stents due to the release of silver ions, which suggested prolonged stent patency, but the clinical efficacy has not been evaluated 13 14 15 16 17 . Recently, the concept of using silver nanoparticles (AgNPs) as an antimicrobial agent in a plastic stent has been studied 18 19 .…”

“…Theoretically, SEMSs covered with silver may have more prolonged stent patency in terms of the prevention of sludge and biofilm formation. However, a preliminary experiment indicated that it is very difficult to coat a AgNP complex on both the external layer and the inner layer of metal stents 17 .…”

Nanoparticles –Photons: Effective or Defective Nanomedicine

“…Darren and Chithrani [6] presented a comprehensive review on applications of nanoparticle in medicine by offering view point on visualization, diagnosis and treatment of disease under a variety of nanoparticles. Yang, et al [7] developed a novel biliary stent.…”

Nanoparticles-Photons: Effective or Defective Nanomedicine

“…Darren & Chithrani [6] presented a comprehensive review on applications of nanoparticle in medicine by offering view point on visualization, diagnosis and treatment of disease under a variety of nanoparticles. Yang et al [7] developed a novel biliary stent.…”