A Novel Bilayer Propionyl-L-carnitine Loaded Polyvinyl Alcohol/Calcium Alginate/Carboxymethyl Cellulose wound dressing for the treatment of diabetic wounds: an in vitro and in vivo study

Abstract

In the current study a drug delivering bilayer porous/nanofibrous wound dressing was developed using a combination of electrospinning and freeze-drying methods. The wound dressings were prepared by lyophilization of 1:1 weight ratios of calcium alginate and carboxymethyl cellulose (CMC) solutions. Drug delivering nanofibrous sheets were fabricated by electrospinning of polyvinyl alcohol (PVA) solution incorporated with 1%,3%,5%, and 10% of Propionyl-L-carnitine. The dressings were studied regarding their microstructure, swelling capacity, mechanical strength, surface wettability, water vapor permeability, drug release profile, in vitro degradation, cell viability assay, hemocompatibility, porosity measurement, microbial penetration assay, and protein adsorption assay. Based on in vitro studies, PVA sheets loaded with 5% Propionyl-L-carnitine was chosen for the preparation of wound dressings. The healing potential of the produced constructs was studied in rat model of diabetic wound. Our results showed that the drug delivering dressings demonstrated significantly higher wound closure and better histological regeneration compared to drug free constructs and sterile gauze. Our results suggest potential applicability of Propionyl-L-carnitine delivering Calcium Alginate/CMC/PVA dressing for the treatment of diabetic wounds in clinic.

Regeneration of sciatic nerve injury through nanofiber neural guidance channels containing collagen hydrogel and acetyl L carnitine: An in vitro and in vivo study

“…It could be that sustained release of ALC from the collagen hydrogel loaded in the NGC’s lumen has resulted in better environment for neural cells growth and function. 41–44 In the HPLT and GMWL experiments, although differences between negative control, NGC, and NGC/hydrogel groups were not significant, the animals treated with the NGCs had better healing response. This result could be due to guiding the axonal sprouts with the NGCs, entrapment of neurotrophic factors, prevention of fibroblast cells infiltration, or cell adhesion sites in the collagen hydrogel that may have promoted the Schwan cells adhesion and proliferation.…”