A novel basydiomycete isolated from mangrove swamps in the Colombian Caribbean shows promise in dye bioremediation

Jutinico-Shubach, Laura Marcela; Castaño, Jesús D.; Juarez, Tulio; Mariño, Miguel Vicente; Gomez‐León, Javier; Blandón, Lina M.

doi:10.1080/10889868.2021.1964426

Cited by 6 publications

(5 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lactobionic acid could be important to lower the pH at early decay in G. trabeum as a complement or alternative to oxalic acid, the most abundant acid found at early decay in R. placenta . At late decay stages, there were several sugar compounds ( 8 ) significantly more abundant in G. trabeum , followed by carboxylic acids ( 4 ), pyranones ( 4 ), furans ( 3 ), and furanones ( 2 ) as the most representative compounds ( Table S3 ). Several of these compounds are the result of the hydrolysis of the lignocellulosic complex.…”

Section: Resultsmentioning

confidence: 99%

“…Contrary to brown rot fungi, P. ostreatus and T. versicolor exhibited a higher number of compounds as significantly more abundant in early wood decay. In the case of P. ostreatus there were 8 sugars and 3 carboxylic acids at higher concentrations at early decay, while T. versicolor showed a more assorted group of compounds such as sugars ( 2 ), carboxylic acids ( 3 ), furans ( 1 ), alcohols ( 1 ), and pyridines ( 1 ) ( Table S3 ). Most of the sugars found in both cases are related to the early degradation of hemicellulose, suggesting (as mentioned before) that white rot fungi are be able to release sugars from hemicellulose earlier than brown rot fungi during wood decay.…”

Section: Resultsmentioning

confidence: 99%

“…Wood-degrading fungi play an essential role in global nutrient cycles as they release large amounts of plant-sequestered carbon (present in the lignocellulose complex) and make available the nutrients bound in plant tissues ( 1 ). Due to their special abilities to decompose the recalcitrant materials present in wood, they have also been used for different applications such as bioremediation, biofuel production, and the pulping industry ( 2 – 5 ), There are different types of wood-degrading organisms with variable abilities to decompose wood, with brown rot and white rot fungi being most common, at least in temperate environments. Brown rot fungi typically degrade wood faster than white rot fungi, rapidly consuming the cellulose and hemicellulose components and primarily modifying and oxidizing lignin without significant removal ( 6 – 9 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Metabolomics Highlights Different Life History Strategies of White and Brown Rot Wood-Degrading Fungi

Castaño

Muñoz-Muñoz

Kim

et al. 2022

mSphere

Self Cite

View full text Add to dashboard Cite

Despite the ecological and economic importance of wood-degrading fungi, little is known about the array of metabolites that fungi produce during wood decomposition. This study provides an in-depth insight into the wood decomposition process by analyzing and comparing the changes of >100 compounds produced by fungi with metabolic distinct nutritional modes (white and brown rot fungi) at different decay stages.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Metabolomics Highlights Different Life History Strategies of White and Brown Rot Wood-Degrading Fungi

Castaño

Muñoz-Muñoz

Kim

et al. 2022

mSphere

Self Cite

View full text Add to dashboard Cite

show abstract

“…Republica de Colombia, DNP and CONPES, 2020). And only a few studies to identify potential uses of the biological diversity in the Caribbean, not specifically in the Atlańtico's marine area, have been conducted such as the ones from (Quintero et al, 2018;Castellanos et al, 2019;Jutinico-Shubach et al, 2021).…”

Section: Marine Bioprospectingmentioning

confidence: 99%

A socio-ecological assessment of land-based contamination and pollution: The Magdalena delta, Colombia

2022

View full text Add to dashboard Cite

River mouth systems, such as estuaries and deltas are important earth system transitional zones linking the land and coastal marine systems. They funnel anthropogenic contaminants and pollutants from the activities in the river catchment into the marine environment. The present study is focused on the Magdalena River-mouth at Bocas de Ceniza where land-based contamination and pollution are important issues. These were subsequently analysed using an expanded DPSIR framework (Drivers Pressures, State, Impacts Responses). Based on export coefficients and secondary information, the loads of BOD, TSS, Nutrients and solid waste from different sources were identified and compared to prioritize their management within the study area. Findings suggest that contaminants generated in the study area may be insignificant compared to the inputs from the Magdalena River, but they can have a significant impact on the state of local ecosystems. Of all the sources analysed, agriculture and livestock are theoretically the largest contributors of nitrogen (i.e., 14.84 t d-1 and 48.99 t d-1) and phosphorus (i.e., 5.90 t d-1 and 19.46 t d-1) in the basin ‘Direct to the Caribbean’ and ‘Cienaga de Mallorquín’ (that altogether are identified as DtC+) and the ‘Western bank of the lower Magdalena’ (DtM-LM), respectively. In the case of BOD, domestic and industrial wastewaters contribute about 5.10 t d-1 and 98.43 t d-1 in the same basins. Contributions of faecal coliforms were more than 4 orders of magnitude higher in untreated wastewater, being 4.86 x1016 NMP d-1 and 1.78 x1016 NMP d-1 in the lower Magdalena and Caribbean catchments, respectively. Inadequately managed waste and plastic represent a serious problem for the Caribbean Basin. Solid waste generated within the study area and coming from the Magdalena basin and the Caribbean Sea, reaches the coast and deteriorates its visual quality, affects mangrove ecosystems, and generates impacts on coastal populations. By basin, the DtM-LM is where the greatest pollutant loads are generated. However, in terms of impact on human well-being, it is the DtC+ that bears the brunt where pressures are translated in impacts such as increased maintenance and cleaning cost of beaches, reduction or loss of tourism revenue, adverse health effects to vulnerable groups, reduce protection against natural hazards, increased living costs and loss of cultural values.

show abstract

“…They selectively and efficiently degrade carbohydrates, while having a low preference for lignin mineralization ( 3 – 6 ). Their distinctive degradation mechanisms thereby hold valuable catalyst tools that can be innovated for the efficient manufacturing of “chemical blocks” and bio-based products ( 7 – 10 ). It has been previously uncovered that fungi achieve this by consolidating the non-enzymatic lignocellulose depolymerization that relies on reactive oxygen species (ROS) for pretreatment attacks (e.g., electrophilic addition or hydrogen abstraction) and the enzymatic degradation that is mediated by glycoside hydrolases to depolymerize poly/oligo-saccharides ( 6 , 11 – 17 ).…”

Section: Introductionmentioning

confidence: 99%

Unlocking the distinctive enzymatic functions of the early plant biomass deconstructive genes in a brown rot fungus by cell-free protein expression

Castaño,

El Khoury,

Goering

et al. 2024

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

Saprotrophic fungi that cause brown rot of woody biomass evolved a distinctive mechanism that relies on reactive oxygen species (ROS) to kick-start lignocellulosic polymers’ deconstruction. These ROS agents are generated at incipient decay stages through a series of redox relays that shuttle electrons from fungus’s central metabolism to extracellular Fenton chemistry. A list of genes has been suggested encoding the enzyme catalysts of the redox processes involved in ROS’s function. However, navigating the functions of the encoded enzymes has been challenging due to the lack of a rapid method for protein synthesis. Here, we employed cell-free expression system to synthesize four redox or degradative enzymes, which were identified, by transcriptomic data, as conserved players of the ROS oxidation phase across brown rot fungal species. All four enzymes were successfully expressed and showed activities that enable confident assignment of function, namely, benzoquinone reductase (BQR), ferric reductase, α-L-arabinofuranosidase (ABF), and heme-thiolate peroxidase (HTP). Detailed analysis of their catalytic features within the context of brown rot environments allowed us to interpret their roles during ROS-driven wood decomposition. Specifically, we validated the functions of BQR as the driver redox enzyme of Fenton cycles and reconstructed its interactions with the co-occurring HTP or laccase and ABF. Taken together, this research demonstrated that the cell-free expression platform is adequate for synthesizing functional fungal enzymes and provided an alternative route for the rapid characterization of fungal proteins, escalating our understanding of the distinctive biocatalyst system for plant biomass conversion. IMPORTANCE Brown rot fungi are efficient wood decomposers in nature, and their unique degradative systems harbor untapped catalysts pursued by the biorefinery and bioremediation industries. While the use of “omics” platforms has recently uncovered the key “oxidative-hydrolytic” mechanisms that allow these fungi to attack lignocellulose, individual protein characterization is lagging behind due to the lack of a robust method for rapid synthesis of crucial fungal enzymes. This work delves into the studies of biochemical functions of brown rot enzymes using a rapid, cell-free expression platform, which allowed the successful depictions of enzymes’ catalytic features, their interactions with Fenton chemistry, and their roles played during the incipient stage of brown rot when fungus sets off the reactive oxygen species for oxidative degradation. We expect this research could illuminate cell-free protein expression system’s use to fulfill the increasing need for functional studies of fungal enzymes, advancing the discoveries of novel biomass-converting catalysts.

show abstract

A novel basydiomycete isolated from mangrove swamps in the Colombian Caribbean shows promise in dye bioremediation

Cited by 6 publications

References 63 publications

Metabolomics Highlights Different Life History Strategies of White and Brown Rot Wood-Degrading Fungi

Metabolomics Highlights Different Life History Strategies of White and Brown Rot Wood-Degrading Fungi

A socio-ecological assessment of land-based contamination and pollution: The Magdalena delta, Colombia

Unlocking the distinctive enzymatic functions of the early plant biomass deconstructive genes in a brown rot fungus by cell-free protein expression

Contact Info

Product

Resources

About