A novel basic helix-loop-helix transcription factor, ZjICE2 from Zoysia japonica confers abiotic stress tolerance to transgenic plants via activating the DREB/CBF regulon and enhancing ROS scavenging

Zuo, Zhi-Fang; Kang, Hong‐Gyu; Hong, Quanchun; Park, Mi-Young; Sun, Hui; Kim, Jeong-Sik; Song, Pill‐Soon; Lee, Hyo-Yeon

doi:10.1007/s11103-019-00957-0

Cited by 22 publications

(11 citation statements)

References 69 publications

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“…Among the 22 GmTLPs , 14 contained ABREs, indicating that these genes can be regulated by ABA. Drought and salt response element MYCs were present in all 22 GmTLP promoter sequences, strongly suggesting that GmTLP members are involved in the responses to those stressors ( Zuo et al, 2020 ). In total, cis -acting element analysis indicated that most members of the GmTLP family may be regulated by ABA in response to drought and salt stresses.…”

Section: Resultsmentioning

confidence: 99%

Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of the GmTLP8 Gene in Abiotic Stress Responses

Liu

et al. 2022

Front. Plant Sci.

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Tubby-like proteins (TLPs) are transcription factors that are widely present in eukaryotes and generally participate in growth and developmental processes. Using genome databases, a total of 22 putative TLP genes were identified in the soybean genome, and unevenly distributed across 13 chromosomes. Phylogenetic analysis demonstrated that the predicted GmTLP proteins were divided into five groups (I-V). Gene structure, protein motifs, and conserved domains were analyzed to identify differences and common features among the GmTLPs. A three-dimensional protein model was built to show the typical structure of TLPs. Analysis of publicly available gene expression data showed that GmTLP genes were differentially expressed in response to abiotic stresses. Based on those data, GmTLP8 was selected to further explore the role of TLPs in soybean drought and salt stress responses. GmTLP8 overexpressors had improved tolerance to drought and salt stresses, whereas the opposite was true of GmTLP8-RNAi lines. 3,3-diaminobenzidine and nitro blue tetrazolium staining and physiological indexes also showed that overexpression of GmTLP8 enhanced the tolerance of soybean to drought and salt stresses; in addition, downstream stress-responsive genes were upregulated in response to drought and salt stresses. This study provides new insights into the function of GmTLPs in response to abiotic stresses.

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Section: Resultsmentioning

confidence: 99%

Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of the GmTLP8 Gene in Abiotic Stress Responses

Liu

et al. 2022

Front. Plant Sci.

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“…1). In zoysiagrass transgenesis, the CaMV 35S or maize ubiquitin promoters have been used to ensure strong expression of genes (Jeong et al 2020;Kim et al 2020;Zuo et al 2020). Here, we used the ubiquitin1 promoter to drive expression casettes in our vectors, since the luciferase activity driven by the control of the maize ubiquitin 1 promoter were found to be relatively higher in zoysiagrass protoplasts than that driven by the control of the 35S promoter (Figs.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the identification of potential genes to improve the economic value of zoysiagrass is becoming important. As genome-wide, high-throughput analysis, such as RNA-seq has enabled the identification of large numbers of genes as genetic resources for genetic manipulation, rapid functional characterization of genes is highly demanded (Ahn et al 2015;Xie et al 2015;Zuo et al 2020). Arabidopsis or rice protoplasts can be used to reveal a regulatory effect of zoysiagrass genes, but their gene function might be misled by different gene repertoires in heterologous systems.…”

Section: Introductionmentioning

confidence: 99%

Transient gene expression system in zoysiagrass leaf mesophyll protoplasts

Kim

Doan

Lee

et al. 2021

Plant Biotechnol Rep

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Transient expression of genes in protoplasts is a versatile technique for rapid functional characterization of genes by assessing protein localization or effector-reporter responses. In addition, protoplasts have been widely used for generating transgenic or gene-edited plants in model or crop plants. Zoysiagrass (Zoysia japonica Steud.) is one of the most economically important turf plants used in many living places or natural fields, but its management is labor-intensive. Therefore, genetic manipulation using valuable genes is highly demanded in zoysiagrass. Although transient expression systems in zoysiagrass facilitates the identification of valuable zoysiagrass genes, transient expression use in the zoysiagrass protoplasts is yet to be reported. Here we describe the methodology and feasibility for transient expression of genes in the zoysiagrass protoplasts isolated from green leaves. We obtained more than 70% of transfection efficiency in the zoysiagrass protoplasts using polyethylene glycolmediated transfection. Additionally, we showed the feasibility of cellular, biochemical, and molecular approaches using the zoysiagrass protoplasts transiently expressing genes of interest through fluorescent microscopic observation, immunoblot analysis, and luciferase-based promoter activity assay. Along with the genome draft of the Zoysia family, transient expression will be a valuable tool to rapidly investigate gene functions. An initial assessment of gene function using protoplasts facilitate the identification of valuable genes that can improve the economic value of the Z. japonica or its closely related species by gene manipulation.

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“…Anthocyanins may scavenge ROS as an efficient antioxidant by neutralizing radicals with their hydroxyl groups to preserve normal cellular redox homeostasis; hence, plants often generate anthocyanins to reduce oxidative damage caused by low-temperature stress [ 20 , 21 , 22 , 23 , 24 , 25 ]. Low-temperature responsive networks in zoysiagrass have been investigated through physiological, biochemical, molecular, genetic and omic approaches [ 7 , 15 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. However, no studies have attempted to associate anthocyanin accumulation with cold tolerance in zoysiagrass.…”

Section: Introductionmentioning

confidence: 99%

A Survey of Enhanced Cold Tolerance and Low-Temperature-Induced Anthocyanin Accumulation in a Novel Zoysia japonica Biotype

Jin

Jiang

Yang

et al. 2022

Plants

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Zoysia japonica is a warm-season turfgrass that is extensively used in landscaping, sports fields, and golf courses worldwide. Uncovering the low-temperature response mechanism of Z. japonica can help to accelerate the development of new cold-tolerant cultivars, which could be used to prolong the ornamental and usage duration of turf. A novel Z. japonica biotype, YueNong-9 (YN-9), was collected from northeastern China for this study. Phenotypic measurements, cold-tolerance investigation, and whole-transcriptome surveys were performed on YN-9 and LanYin-3 (LY-3), the most popular Z. japonica cultivar in Southern China. The results indicated the following: YN-9 has longer second and third leaves than LY-3; when exposed to the natural low temperature during winter in Guangzhou, YN-9 accumulated 4.74 times more anthocyanin than LY-3; after cold acclimation and freezing treatment, 83.25 ± 9.55% of YN-9 survived while all LY-3 leaves died, and the dark green color index (DGCI) value of YN-9 was 1.78 times that of LY-3; in YN-9, there was a unique up-regulation of Phenylalanine ammonia-lyase (PAL), Homeobox-leucine Zipper IV (HD-ZIP), and ATP-Binding Cassette transporter B8 (ABCB8) expressions, as well as a unique down-regulation of zinc-regulated transporters and iron-regulated transporter-like proteins (ZIPs) expression, which may promote anthocyanin biosynthesis, transport, and accumulation. In conclusion, YN-9 exhibited enhanced cold tolerance and is thus an excellent candidate for breeding cold-tolerant Z. japonica variety, and its unique low-temperature-induced anthocyanin accumulation and gene responses provide ideas and candidate genes for the study of low-temperature tolerance mechanisms and genetic engineering breeding.

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A novel basic helix-loop-helix transcription factor, ZjICE2 from Zoysia japonica confers abiotic stress tolerance to transgenic plants via activating the DREB/CBF regulon and enhancing ROS scavenging

Cited by 22 publications

References 69 publications

Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of the GmTLP8 Gene in Abiotic Stress Responses

Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of the GmTLP8 Gene in Abiotic Stress Responses

Transient gene expression system in zoysiagrass leaf mesophyll protoplasts

A Survey of Enhanced Cold Tolerance and Low-Temperature-Induced Anthocyanin Accumulation in a Novel Zoysia japonica Biotype

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