A novel bacteriocin from Lactobacillus salivarius against Staphylococcus aureus: Isolation, purification, identification, antibacterial and antibiofilm activity

Li, Hongwei; Xiang, Yiqiang; Zhang, Man; Jiang, Yu-Hang; Zhang, Yao; Liu, Ying-Yang; Lü, Lin; Zhang, Qilin

doi:10.1016/j.lwt.2020.110826

Cited by 45 publications

(44 citation statements)

References 51 publications

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“…Lactobacillus spp. are important probiotics (Anderson et al 2011;Vasquez et al 2012), and they can secrete bacteriostatic substances (bacteriocins and lactic acid) to protect their hosts (De Keersmaecker et al 2006;Li et al 2021). Recently, Suphaphimol et al (2020) isolated lactic acid bacteria from the gut of L. terminata that were antagonistic to pathogens of honeybees (Paenibacillus larvae).…”

Section: Ve Nt Ra Lis T P a G D En I L Ter M In At Amentioning

confidence: 99%

The composition of bacteria in gut and beebread of stingless bees (Apidae: Meliponini) from tropics Yunnan, China

Tang

Miao

Chen

et al. 2021

Antonie van Leeuwenhoek

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Stingless bees are the main pollinators in tropical and subtropical regions. However, there are only a few studies on the structure and composition of bacteria in the gut and beebread of stingless bees, especially in China. To address this shortage of information, we characterized the microbiota of three common species of stingless bees (Lepidotrigona terminata, Lepidotrigona ventralis and Tetragonula pagdeni) and beebread samples of T. pagdeni. The results showed that the gut of stingless bees contained a set of dominant bacteria, including Acetobacter-like, Snodgrassella, Lactobacillus, Psychrobacter, Pseudomonas, Bifidobacterium and other species. The gut microbiota structures of the three stingless bees were different, and the abundances of bacterial species in the gut varied between communities of the same bee species. The reasons for this are manifold and may include food preference, age and genetic differences. In addition, the abundances of Lactobacillus, Carnimonas, Escherichia-Shigella, Acinetobacter and other species were high in the beebread of stingless bees. In conclusion, our findings reveal the bacteria composition and structure of the gut and beebread of stingless bees in China and deepen our understanding of the dominant bacteria of the gut and beebread of stingless bees.

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Section: Ve Nt Ra Lis T P a G D En I L Ter M In At Amentioning

confidence: 99%

The composition of bacteria in gut and beebread of stingless bees (Apidae: Meliponini) from tropics Yunnan, China

Tang

Miao

Chen

et al. 2021

Antonie van Leeuwenhoek

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“…Bacteriocins of gram-positive bacteria have different transport mechanism to encode to release bacteriocin toxins [110]. More recently, Li et al [111] purified a novel bacteriocin designated as XJS01 was obtained from Lactobacillus salivarius CGMCC2070 LAB strain demonstrated antibacterial and antibiofilm applications against Staphylococcus aureus. Antimicrobial activity and molecular mass composition of XJS01 were 666.31 Da and contain F-S-G-L-A-G-D amino acid, which could inhibit the growth of S. aureus strain 2612:1606BL1486 (S. aureus 26) in chicken meat up to 9.85 μg mL -1 inhibitory concentrations.…”

Section: Classification Of Bacteriocinsmentioning

confidence: 99%

Recent biotechnological trends in lactic acid bacterial fermentation for food processing industries

Raj

Chandrasekhar

Kumar

et al. 2021

Syst Microbiol and Biomanuf

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Lactic acid bacteria (LAB) are non-mobile, gram-positive, non-spore-forming, micro-aerophilic microorganisms widely explored as starter cultures food industry to enhance the gustatory, nutritional value, imparts appetizing flavour, texture to milk, vegetative, meat foods and prolongs their shelf life. This vast review emphasis various LABs widely explored in the food industry. Herein, we have summarized the classification of LAB strains, their metabolic pathways for biosynthesis of lactic acid, ethanol, acetic acid and demonstrated their application in various food industries for making fermented milk (yoghurt), cheese, beverages, bread, and animal foods. The wide spectrum of LAB-based probiotics, bacteriocins, exopolysaccharides, bio preservative and their relevant benefits towards human health has also been discussed. Moreover, LAB bacteriocins and probiotics in food application may limit the growth of pathogenic, while boosting health immunity. Microbial exopolysaccharides have interesting characteristics for the fermented food industry as new functional foods. Later on, we have discussed the various advancement in metabolic engineering, synthetic biology tools, which have gained considerable interest to elucidate the biosynthetic pathway for tailoring cellular metabolism for high activity.

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“…Furthermore, high-density structure of the biofilm protects pathogenic bacteria from acidic and antibiotic treatments ( Tsukatani et al, 2020 ). In particular, cells of pathogenic bacteria in biofilms are 10–1,000 times less sensitive to disinfectants than planktonic cells ( Li et al, 2021 ; Xiang et al, 2021 ). Moreover, biofilm is one of the primary causes of equipment damage, thus leading to increased energy cost, as well as is involved in the occurrence of food spoilage and foodborne diseases ( Nové et al, 2020 ; Tsukatani et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…In particular, many lactic acid bacteria (LAB) bacteriocins have been demonstrated with high safety and tolerance to heat, acids, and bases ( Guo et al, 2020 ). For instance, LAB bacteriocins derived from Lactococcus lactis 2Mt and Lactobacillus salivarius CGMCC20700 were found to effectively inhibit foodborne Staphylococcus aureus under a wide range of acid-base and temperature ( Pmka et al, 2019 ; Li et al, 2021 ). Although many LAB bacteriocins have been identified so far, their effects on biofilm formation of foodborne S. flexneri are still unexplored.…”

Section: Introductionmentioning

confidence: 99%

A Novel Bacteriocin Against Shigella flexneri From Lactiplantibacillus plantarum Isolated From Tilapia Intestine: Purification, Antibacterial Properties and Antibiofilm Activity

et al. 2022

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Few bacteriocins with antibacterial activity against Shigella flexneri have been reported. Here, a novel bacteriocin (LFX01) produced by Lactiplantibacillus plantarum strain LF-8 from the intestine of tilapia was purified and extensively characterized. LFX01 possesses a molecular weight of 1049.56 Da and an amino acid sequence of I-T-G-G-P-A-V-V-H-Q-A. LFX01 significantly inhibited S. flexneri strain 14 (S. flexneri_14) growth. Moreover, it exhibited excellent stability under heat and acid-base stress, and presented sensitivity to a variety of proteases, such as proteinase K, pepsin, and trypsin. The minimum inhibitory concentration (MIC) of LFX01 against S. flexneri_14 was 12.65 μg/mL, which was smaller than that of most of the previously found bacteriocins. Furthermore, LFX01 significantly inhibited (p < 0.05) S. flexneri_14 cells and decreased their cell viability. In addition, LFX01 could significantly (p < 0.05) inhibit biofilm formation of S. flexneri_14. Scanning electron microscopy analysis presented that the cell membrane permeability of S. flexneri_14 was demolished by LFX01, leading to cytoplasmic contents leakage and cell rupture death. In summary, a novel bacteriocin of lactic acid bacteria (LAB) was found, which could effectively control S. flexneri in both planktonic and biofilm states.

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A novel bacteriocin from Lactobacillus salivarius against Staphylococcus aureus: Isolation, purification, identification, antibacterial and antibiofilm activity

Cited by 45 publications

References 51 publications

The composition of bacteria in gut and beebread of stingless bees (Apidae: Meliponini) from tropics Yunnan, China

The composition of bacteria in gut and beebread of stingless bees (Apidae: Meliponini) from tropics Yunnan, China

Recent biotechnological trends in lactic acid bacterial fermentation for food processing industries

A Novel Bacteriocin Against Shigella flexneri From Lactiplantibacillus plantarum Isolated From Tilapia Intestine: Purification, Antibacterial Properties and Antibiofilm Activity

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