A novel B cell population revealed by a CD38/CD24 gating strategy: CD38−CD24− B cells in centenarian offspring and elderly people

Buffa, Silvio; Pellicanò, Mariavaleria; Bulati, Matteo; Martorana, Adriana; Goldeck, David; Caruso, Calogero; Pawelec, Graham; Colonna‐Romano, Giuseppina

doi:10.1007/s11357-012-9488-5

Cited by 63 publications

(58 citation statements)

References 56 publications

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“…2a). A novel, potentially important TNF-producing CD24-CD38- B-cell population has been described by Buffa et al [17] and gated here (online suppl. fig.…”

Section: Resultsmentioning

confidence: 89%

Cytomegalovirus Infection Minimally Affects the Frequencies of B-Cell Phenotypes in Peripheral Blood of Younger and Older Adults

Goldeck¹,

Oettinger²,

Janssen³

et al. 2016

Gerontology

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Background: An accumulation of late-differentiated CD8+ T-cells together with fewer B-cells and seropositivity for cytomegalovirus (CMV) characterises an ‘immune risk profile' associated with mortality in elderly people and represents one of the hallmarks of ‘immunosenescence'. Objectives: While differences in memory T-cell phenotypes between young and old people have been intensively studied, and the role of CMV is well-accepted as a driving force in this regard, the impact of CMV on B-cells, if any, has been relatively neglected thus far. Methods: Here, we avail ourselves of blood samples from participants of the Berlin Aging Study II (BASE-II) to compare peripheral blood B-cell differentiation phenotypes of 140 age- and gender-matched CMV-seronegative or -seropositive adults aged between 24 and 85 years using multicolour flow cytometry analysis. Results: We found that the frequencies of naïve B-cells within the CD19+ population were not significantly different in younger and older CMV-seronegative people. This was also true in CMV-seropositive subjects. The frequencies of late-differentiated B-cells were also not different in CMV-negative elderly and young. However, in marked contrast to the T-cell compartment, this was also true for late differentiated B-cells. Within age groups, the most marked differences in the distribution of B-cell phenotypes were between CMV-seronegative and -seropositive subjects, for both genders. Conclusion: These results emphasize the importance of including CMV serostatus in the analysis of immune signatures. Because the proportion of the population infected with CMV increases with age, the effect of CMV rather than age could confound analyses seeking age-associated changes to human immunity.

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“…2a). A novel, potentially important TNF-producing CD24-CD38- B-cell population has been described by Buffa et al [17] and gated here (online suppl. fig.…”

Section: Resultsmentioning

confidence: 89%

Cytomegalovirus Infection Minimally Affects the Frequencies of B-Cell Phenotypes in Peripheral Blood of Younger and Older Adults

Goldeck¹,

Oettinger²,

Janssen³

et al. 2016

Gerontology

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“…We have previously demonstrated that DN B cells from young and elderly donors are differently activated both in terms of proliferation [10] and cytokine production [50,51]. In recent years, Moir et al [23] have described the increase of an exhausted B cell population, named "tissue-like" B cells, in HIV-viraemic patients.…”

Section: Discussionmentioning

confidence: 99%

Double negative (CD19+IgG+IgD−CD27−) B lymphocytes: A new insight from telomerase in healthy elderly, in centenarian offspring and in Alzheimer's disease patients

et al. 2014

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a b s t r a c tImmunosenescence is characterized by the impairment of humoral immunity with changes in the memory/naive B cell compartment. In particular we have previously reported the percentage increase of a Memory IgD − CD27 − (Double Negative, DN) B cell population in aged people. In this study, we have further characterized DN B cells with the aim to better understand their contribution to immunosenescence. As DN B cells show a poor ability to proliferate in vitro, we have evaluated the expression of the inhibitory receptors CD307d and CD22 on these cells from young and old individuals. In addition we have evaluated the ability to activate DN B cells by the simultaneous use of innate (CpG) and adaptive (␣-Ig/CD40) ligands. Our data demonstrate that the refractoriness to proliferate of DN B cells does not depend on the expression of inhibitory receptors, but it is due to the kind of stimulation. Indeed, when DN B cells are stimulated engaging both BCR and TLR9, they become able to proliferate and reactivate the telomerase enzyme. In the present study, we have also compared the telomerase activity in a group of people genetically advantaged for longevity as centenarian offspring (CO) and in a group of moderate-severe Alzheimer's disease (AD) patients, who represent a model of unsuccessful aging. Our study suggests that telomerase reactivation of DN B cells, as well as their number and ability in activating, depend essentially by the biological age of the subjects studied, so the evaluation of DN B cells might allow to gain insight to healthy and unsuccessful aging.

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“…They still express CD10, co-express IgD and IgM and present an activated phenotype with high expression of CD24 and CD38. [3][4][5] These cells home mainly to the spleen where they differentiate into CD27+IgM+ memory B-cells. 3 Immature-transitional B-cell frequencies are high in healthy children and slowly decrease with age until stable range levels are reached around 13 y of age.…”

Section: Overview On B-cell Development and Functionmentioning

confidence: 99%

“…31 Interestingly, B-cell immunophenotyping based on the activation markers CD24 and CD38 also revealed another population of CD24-CD38-B-cells to be increased in elderly individuals. 5 These cells produce the proinflammatory cytokine TNF-α which might contribute to the inflammatory status often observed in the elderly. 5 A summary of the effect of healthy aging on the different B-cell subpopulations has been incorporated in Table 1.…”

Section: B-cells During Healthy Agingmentioning

confidence: 99%

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Premature B-cell senescence as a consequence of chronic immune activation

Rinaldi

Cotugno

Santilli

et al. 2014

Human Vaccines & Immunotherapeutics

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A novel B cell population revealed by a CD38/CD24 gating strategy: CD38−CD24− B cells in centenarian offspring and elderly people

Cited by 63 publications

References 56 publications

Cytomegalovirus Infection Minimally Affects the Frequencies of B-Cell Phenotypes in Peripheral Blood of Younger and Older Adults

Cytomegalovirus Infection Minimally Affects the Frequencies of B-Cell Phenotypes in Peripheral Blood of Younger and Older Adults

Double negative (CD19+IgG+IgD−CD27−) B lymphocytes: A new insight from telomerase in healthy elderly, in centenarian offspring and in Alzheimer's disease patients

Premature B-cell senescence as a consequence of chronic immune activation

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