A Novel Aux/IAA28 Signaling Cascade Activates GATA23-Dependent Specification of Lateral Root Founder Cell Identity

Rybel, Bert De; Vassileva, Valya; Parizot, Boris; Demeulenaere, Marlies; Grunewald, Wim; Audenaert, Dominique; Campenhout, Jelle Van; Overvoorde, Paul J.; Jansen, Leentje; Vanneste, Steffen; Möller, Barbara; Wilson, Michael; Holman, Tara J.; Isterdael, Gert Van; Brunoud, Géraldine; Vuylsteke, Marnik; Vernoux, Teva; Veylder, Lieven De; Inzé, Dirk; Weijers, Dolf; Bennett, Malcolm J.; Beeckman, Tom

doi:10.1016/j.cub.2010.09.007

Cited by 434 publications

(518 citation statements)

References 36 publications

Supporting

Mentioning

501

Contrasting

Unclassified

Order By: Relevance

“…We observed that factors regulating auxin signaling, such as SOLITARY ROOT (IAA14), could be also involved in de novo root initiation; we detected decreased root capacity for slr-1, which could be indicative of fewer primordia initiation. In addition, the IAA28 module, which is upstream of lateral root founder cell specification (De Rybel et al, 2010), also regulates de novo root founder cell specification or initiation, although further experiments could dissect more precisely at which stage IAA28 is involved. Our results show that factors primarily involved in formation of lateral roots are also affected in rooting of leaves, suggesting the existence of partially overlapping auxin signaling modules during postembryonic root development.…”

Section: Root Primordia Initiation and Patterningmentioning

confidence: 99%

Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis

et al. 2017

View full text Add to dashboard Cite

Body regeneration through formation of new organs is a major question in developmental biology. We investigated de novo root formation using whole leaves of Arabidopsis (Arabidopsis thaliana). Our results show that local cytokinin biosynthesis and auxin biosynthesis in the leaf blade followed by auxin long-distance transport to the petiole leads to proliferation of J0121-marked xylem-associated tissues and others through signaling of INDOLE-3-ACETIC ACID INDUCIBLE28 (IAA28), CRANE (IAA18), WOODEN LEG, and ARABIDOPSIS RESPONSE REGULATORS1 (ARR1), ARR10, and ARR12. Vasculature proliferation also involves the cell cycle regulator KIP-RELATED PROTEIN2 and ABERRANT LATERAL ROOT FORMATION4, resulting in a mass of cells with rooting competence that resembles callus formation. Endogenous callus formation precedes specification of postembryonic root founder cells, from which roots are initiated through the activity of SHORT-ROOT, PLETHORA1 (PLT1), and PLT2. Primordia initiation is blocked in shr plt1 plt2 mutant. Stem cell regulators SCHIZORIZA, JACKDAW, BLUEJAY, and SCARECROW also participate in root initiation and are required to pattern the new organ, as mutants show disorganized and reduced number of layers and tissue initials resulting in reduced rooting. Our work provides an organ regeneration model through de novo root formation, stating key stages and the primary pathways involved.Plants have striking regeneration capacities, and can produce new organs from postembryonic tissues (Hartmann et al., 2010;Chen et al., 2014;Liu et al., 2014) as well as reconstitute damaged organs upon wounding (Xu et al., 2006;Heyman et al., 2013; PerianezRodriguez et al., 2014;Melnyk et al., 2015;Efroni et al., 2016). Intriguingly, root regeneration upon stem cell damage recruits embryonic pathways (Hayashi et al., 2006;Efroni et al., 2016), whereas in contrast, postembryonic formation of whole new organs, such as lateral roots, appears to use specific postembryonic pathways (Lavenus et al., 2013).Cross talk between auxin and cytokinin signaling is required for many aspects of plant development and regeneration (El-Showk et al., 2013), although how their synergistic interaction is implemented at the molecular level has not been clarified (Skoog and Miller, 1957;Chandler and Werr, 2015). Exogenous in vitro supplementation of these two hormones results in continuous cell proliferation, to form a characteristic structure termed "callus". Callus emerges as a common regenerative mechanism for almost all plant organs through in vitro culture (Atta et al., 2009;Sugimoto et al., 2010). There is increasing evidence that callus formation requires hormone-mediated activation of a lateral and meristematic root development program in pericycle-like cells defined by expression of the J0121 marker 1 This work was supported by grants from Ministerio de Economía y Competitividad (MINECO) of Spain, the European Regional Development Fund (ERDF) and FP7 Funds of the European Commission, BFU2013-41160-P, BFU2016-80315-P, and PCIG11-GA-2012-322082 to M.A....

show abstract

Section: Root Primordia Initiation and Patterningmentioning

confidence: 99%

Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis

et al. 2017

View full text Add to dashboard Cite

show abstract

“…1D and 2C). The altered root pattern could originate at the level of lateral root founder cell specification, which occurs in the basal meristem before the initial anticlinal division of the founder cells (De Rybel et al, 2010). To visualize the effect of c-CA on lateral root priming, we used a reporter line harboring the promoter of the GATA23 transcription factor fused to a GUS reporter.…”

Section: Ca Affects Plant Developmentmentioning

confidence: 99%

“…To visualize the effect of c-CA on lateral root priming, we used a reporter line harboring the promoter of the GATA23 transcription factor fused to a GUS reporter. GATA23 expression is considered as hallmark of the earliest steps in lateral root formation (De Rybel et al, 2010). In mock-treated plants, GUS expression was observed in pericycle cells starting close to the root tip and continued along the root in a zone lacking emerged lateral root primordia.…”

Section: Ca Affects Plant Developmentmentioning

confidence: 99%

“…The c-isomer is the biologically active isomer affecting a number of developmental processes in planta, and it is likely that most if not all physiological effects that have been previously attributed to the t-CA isomer or CA in general are caused by c-CA. In Arabidopsis, lateral roots arise from asymmetric anticlinal divisions of founder cells in the pericycle layer basal to the main root meristem (De Rybel et al, 2010). As c-CA causes lateral root proliferation (Fig.…”

Section: Ca Affects Plant Developmentmentioning

confidence: 99%

“…Arabidopsis ecotype Columbia (Col-0) was used, unless stated elsewhere. Following transgenic lines were in the same ecotype: DII-VENUS, pDR5rev:GFP, pDR5:LUC, pGATA23:GUS, pGAZAT:GUS, pKNOLLE:KNOLLE-GFP, p35S:iaaL, slr, arf7 arf19, and tir1 afb2 afb3 (Romano et al, 1991;Lukowitz et al, 1996;Fukaki et al, 2002;Friml et al, 2003;Dharmasiri et al, 2005;González-Carranza et al, 2007;Okushima et al, 2007;De Rybel et al, 2010;Moreno-Risueno et al, 2010;Brunoud et al, 2012). The transgenic line pCYCB1:GUS was in the ecotype Landsberg erecta (Ler; Colón-Carmona et al, 1999).…”

Section: Plant Materials Transgenic Lines Chemicals and Growth Condmentioning

confidence: 99%

See 2 more Smart Citations

cis-Cinnamic Acid Is a Novel, Natural Auxin Efflux Inhibitor That Promotes Lateral Root Formation

et al. 2016

View full text Add to dashboard Cite

Pericycle

Dubrovsky¹,

Rost²

2012

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

Pericycle is a primary tissue of plant roots and is the site for the initiation of lateral roots and two secondary meristems, the vascular cambium and cork cambium (phellogen). In this article the characteristics of the pericycle related to each of its functions are reviewed. During lateral root development, three important and coordinated events take place in the pericycle: priming, founder cells specification and patterned cell division leading to primordium formation. The vascular cambium is initiated from the pericycle, just outside the primary xylem, and from residual procambium that originates from vascular parenchyma cells located between the primary xylem and primary phloem resulting in a continuous cylinder of vascular cambium cells which form the secondary xylem and secondary phloem of the root. The pericycle tends to be ‘pushed’ outwards after the vascular cambium forms and it eventually divides again to become the cork cambium. The cork cambium divides to form phellem cells to the outside and phelloderm to the inside. Aspects of the molecular mechanisms involved in the development and function of the pericycle and its derivatives are also discussed. Key Concepts: Pericycle is a primary tissue located at the periphery (most outward) of the root vascular cylinder. In roots, the pericycle is a unique tissue in that its cells continue to cycle for a long period and it has three functions – initiation of lateral roots, contribution to the initiation of the vascular cambium and initiation of the cork cambium. Lateral root initiation involves the following steps: pericycle cell priming that occurs in a so‐called oscillation zone, founder cell specification that is dependent on local increase in auxin concentration and first divisions of founder cells leading to primordium formation. Auxin is a key hormone involved in the regulation of pericycle activity related to lateral root formation but it interacts and acts in a complex orchestration with other plant hormones. Auxin homeostasis‐ and signalling‐related genes are involved in pericycle cell development, lateral root initiation and patterned division of founder cells during lateral root formation; some auxin‐independent genes are also important for pericycle development. Vascular cambium forms by division of the pericycle cells just outside the primary xylem and also by division of residual procambium cells located between the primary xylem and primary phloem. Vascular cambium divides toward the inside of the root to form secondary xylem and towards the outside to form the secondary phloem; auxins and cytokinins are essential to initiate the vascular cambium. The cork cambium, or phellogen, forms from the pericycle outside the secondary phloem. It divides to the outside to form a layer of cells called phellem and to the inside to form the phelloderm. The cell walls of these cell layers are impregnated with a waxy substance called suberin to retard water loss out of the root and to prevent the entry of pathogens.

show abstract

A Novel Aux/IAA28 Signaling Cascade Activates GATA23-Dependent Specification of Lateral Root Founder Cell Identity

Cited by 434 publications

References 36 publications

Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis

Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis

cis-Cinnamic Acid Is a Novel, Natural Auxin Efflux Inhibitor That Promotes Lateral Root Formation

Pericycle

Contact Info

Product

Resources

About