A Novel Atopic Dermatitis Model Induced by Topical Application with Dermatophagoides Farinae Extract in NC/Nga Mice

Yamamoto, Mina; Haruna, Takayo; Yasui, Kiyoshi; Takahashi, Hisashi; Iduhara, Miho; Takaki, Shigeki; Deguchi, Masashi; Arimura, Akinori

doi:10.2332/allergolint.o-06-458

Cited by 185 publications

(191 citation statements)

References 21 publications

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“…HDM was assumed to act not only as an allergen but also as a nonspecific irritant in AD skin lesions via a proteolytic activity of the HDM allergens. [25][26][27] In conclusion, this pilot study revealed that 24% of a group of allergic children from Cairo were sensitized to one or more of five strains of HDM, D. pteronyssinus and D. farinae being the most common. Testing children with several mite species would give information on the varied geographic distribution and might help efforts to develop more targeted specific immunotherapy.…”

Section: Discussionmentioning

confidence: 99%

Sensitivity to Five Types of House Dust Mite in a Group of Allergic Egyptian Children

Hossny

El-Sayed

Abdul-Rahman

2014

Pediatric Allergy, Immunology, and Pulmonology

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Background: The published data on house dust mite (HDM) sensitization from Egypt are scanty. We sought to investigate the sensitization to five different types of HDM among a group of allergic children in a trial to outline the most frequent sensitizing strains in the Cairo Province. Methods: We consecutively enrolled 100 asthmatic patients, aged 1-7 years, of whom 22 had concomitant skin allergy. Skin prick testing was performed using allergen extracts of Dermatophagoides pteronyssinus, Dermatophagoides farinae, Lepidoglyphus destructor, Tyrophagus putrescentiae, and Acarus siro. Results: Twenty-four patients (24%) were sensitized to one or more strains of HDM. Sensitization to one strain was revealed in 12% of the studied sample, while sensitization to two or three strains was detected in 8% and 4% respectively. Twelve percent of the enrolled children were sensitive to D. pteronyssinus, 11% to D. farinae, 7% to L. destructor, 6% to T. putrescentiae, and 4% to A. siro. Eight out of the 12 (66%) children sensitive to one strain had mild intermittent asthma, while five out of eight (62.5%) sensitive to two strains had moderate persistent asthma. All children sensitized to three strains of HDM had persistent rather than intermittent asthma. HDM sensitization did not correlate significantly to the history of sun exposure, bed mattresses and pillows, living in farms, or exposure to stored grains. The co-existence of atopic dermatitis tended to have a higher rate of HDM sensitization. Conclusion: D. pteronyssinus and D. farinae represent the most common sensitizing strains in the studied sample. Wider-scale population-based studies are needed to assess the prevalence of HDM allergy and its clinical correlates in our country.

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Section: Discussionmentioning

confidence: 99%

Sensitivity to Five Types of House Dust Mite in a Group of Allergic Egyptian Children

Hossny

El-Sayed

Abdul-Rahman

2014

Pediatric Allergy, Immunology, and Pulmonology

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“…NC/Nga mice were challenged with Dfb ointment containing mite extract on the dorsal lesion; 2 wk later, these mice gradually began to develop symptoms of AD, including dryness, edema, and hemorrhage (28). Because P6 alone was difficult to dissolve in PBS at high concentrations, we developed P6-nano.…”

Section: P6-nano Treatment Ameliorates Admentioning

confidence: 99%

Pyridone 6, a Pan-JAK Inhibitor, Ameliorates Allergic Skin Inflammation of NC/Nga Mice via Suppression of Th2 and Enhancement of Th17

Nakagawa

Yoshida

Asakawa

et al. 2011

The Journal of Immunology

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Atopic dermatitis (AD) is a common pruritic inflammatory disease triggered by a defective skin barrier and immunodysregulation. AD has been considered a typical example of a Th2 response associated with allergic disease. In the early phases of the disease, symptoms include IgE hyperproduction, eosinophil accumulation, and mast cell activation; in the chronic phase, a Th1-dominant immune response is also observed at the sites of AD skin lesions. The role of IL-17–producing Th (Th17) cells in AD has not been established. In the current study, we found that pyridone 6 (P6), a pan-JAK inhibitor, delayed the onset and reduced the magnitude of skin disease in an AD-like skin-disease model of NC/Nga mice. P6 reduced IFN-γ and IL-13, whereas it enhanced IL-17 and IL-22 expression. In vitro, P6 also inhibited both Th1 and Th2 development, whereas it promoted Th17 differentiation from naive T cells when present within a certain range of concentrations. This was probably because P6 strongly inhibited STAT1, STAT5, and STAT6 phosphorylation, whereas STAT3 phosphorylation was less efficiently suppressed by P6 at the same concentration. Furthermore, IL-22 protects keratinocytes from apoptosis induced by IFN-γ, and administration of IL-17 and IL-22 partially ameliorated skin diseases in NC/Nga mice. These results suggested that the JAK inhibitor P6 is therapeutic for AD by modulating the balance of Th2 and Th17.

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“…NC/Nga mice는 태어난 후 일반사육환경 (conventional system)에서 노출되면 사람의 아 토피피부염과 유사한 병변의 형태 즉, 딱지가 앉은 상처 및 작은 결정의 병변이 관찰되는 동물로서 아토피피부염 실험 동물로 사용되고 있다 [17]. Mite extract (Biostir mite extract antigen cream, BMAC)은 Dermatophagoides farinae crude extract (mite extract antigen, lyophilized)를 진드기 항원으로 제조된 것으로 진드기 항원 tween 20이 0.5% 함유된 ointment base로 제작된 것을 사용하였다 (Biostir, Hiroshima, Japan) [18]. IgE, IgG1 ELISA kit는 SHIBAYAGI사 (Shibukawa, Japan)제품, γ-PGA는 바이웰㈜에서 구입하였으며 평균 분자 량은 300 kDa이다.…”

Section: Methodsunclassified

XS-E is Induced Atopic Dermatitis NC/Nga Mice the Impact of Skin Conditions

김금란¹

2014

KSBB Journal

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This study reports significant improvement of atopic dermatitis condition as a result of experiment using Xanthium strumarium L. extract (XS-E) at the dorsal skin of induced atopic dermatitis Nc/Nga mice. Skin clinical score has decreased (2.75±0.85, *p<0.05), showing visible change of skin condition. IgE (***p<0.001) and IgG1 (2522.00±32.80, ***p<0.001) in plasma also decreased significantly. mRNA (gene expression) level increased (RQ=2.75±0.10, ***p<0.001) within skin tissue of CD4+CD25+Foxp3+ Treg cell that's activated by XS-E dosage, thereby discovering that there is an effect of suppressing proliferation and viability of Th2 cell, eosinophils, mast cell and inflammatory cell. Upon examining cells permeated with H&E and toluidine blue staining technique, thickness of epidermis and mast cell's permeation decreased, and the result of examining the distribution of CCR 3+ eosinophils within ALN showed that it's level fell down to that of wild type (normal group, NC/Nga-WT). By such results, it is suggested that XS-E is highly effective on atopic dermatitis, and it is considered that continued quantitative research and case study of clinical research such as effect of cell number in individual tissues or change of total cell number are necessary.

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A Novel Atopic Dermatitis Model Induced by Topical Application with Dermatophagoides Farinae Extract in NC/Nga Mice

Abstract: These results suggest that the model we have established is useful for not only elucidating the pathogenesis of atopic dermatitis but also for evaluating therapeutic agents.

Cited by 185 publications

References 21 publications

Sensitivity to Five Types of House Dust Mite in a Group of Allergic Egyptian Children

Sensitivity to Five Types of House Dust Mite in a Group of Allergic Egyptian Children

Pyridone 6, a Pan-JAK Inhibitor, Ameliorates Allergic Skin Inflammation of NC/Nga Mice via Suppression of Th2 and Enhancement of Th17

XS-E is Induced Atopic Dermatitis NC/Nga Mice the Impact of Skin Conditions

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