A Novel Arthropod Host of Brucellosis in the Arid Steppe Ecosystem

Huang, Tianpeng; Zhang, Jinbao; Sun, Changyun; Liu, Zhicheng; He, Haiyan; Wu, Jie; Geriletu,

doi:10.3389/fvets.2020.566253

Cited by 13 publications

(15 citation statements)

References 31 publications

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“…3 ). According to Huang et al [ 62 ], they were able to use a fluorescent quantitative PCR technique to detect the copy number of the Bcsp31 gene of Brucella in the salivary gland and midgut of D. nuttalli . The study was successful in detecting the BCSP31 protein of Brucella at the protein level as well.…”

Section: Resultsmentioning

confidence: 99%

Evidence-practice gap analysis in the role of tick in brucellosis transmission: a scoping review

Ma,

Li,

Gao

et al. 2024

Infect Dis Poverty

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Background Brucellosis is a zoonotic affliction instigated by bacteria belonging to the genus Brucella and is characterized by a diverse range of pervasiveness, multiple transmission routes, and serious hazards. It is imperative to amalgamate the current knowledge and identify gaps pertaining to the role of ticks in brucellosis transmission. Methods We systematically searched China National Knowledge Infrastructure (CNKI), WanFang, Google Scholar, and PubMed on the topic published until April 23, 2022. The procedure was performed in accordance with the Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) guidelines. The selected articles were categorized across three major topic areas, and the potential data was extracted to describe evidence-practice gaps by two reviewers. Results The search identified 83 eligible studies for the final analyses. The results highlighted the potential capacity of ticks in brucellosis transmission as evidenced by the detection of Brucella in 16 different tick species. The pooled overall prevalence of Brucella in ticks was 33.87% (range: 0.00–87.80%). The review also revealed the capability of Brucella to circulate in parasitic ticks' different developmental stages, thus posing a potential threat to animal and human health. Empirical evidence from in vitro rodent infection experiments has revealed that ticks possess the capability to transmit Brucella to uninfected animals (range: 45.00–80.00%). Moreover, significant epidemiological associations have been found between the occurrence of brucellosis in animals and tick control in rangelands, which further suggests that ticks may serve as potential vectors for brucellosis transmission in ruminants. Notably, a mere three cases of human brucellosis resulting from potential tick bites were identified in search of global clinical case reports from 1963 to 2019. Conclusions It is imperative to improve the techniques used to identify Brucella in ticks, particularly by developing a novel, efficient, precise approach that can be applied in a field setting. Furthermore, due to the lack of adequate evidence of tick-borne brucellosis, it is essential to integrate various disciplines, including experimental animal science, epidemiology, molecular genetics, and others, to better understand the efficacy of tick-borne brucellosis. By amalgamating multiple disciplines, we can enhance our comprehension and proficiency in tackling tick-borne brucellosis.

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Section: Resultsmentioning

confidence: 99%

Evidence-practice gap analysis in the role of tick in brucellosis transmission: a scoping review

Ma,

Li,

Gao

et al. 2024

Infect Dis Poverty

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“…Dermacentor nuttalli is a potent, long-term carrier of Brucella spp. and shows transovarial transmission potential (34), indicating the potential risk of transmission of brucellosis between animals and humans via tick bites. Staphylococcus xylosus is a commensal grampositive bacterium found on human and animal skin and is a clinically relevant pathogen in veterinary medicine (91).…”

Section: Discussionmentioning

confidence: 99%

“…The V3-V4 hypervariable regions are the most accurate for identifying organisms at the genus level (14). Previous studies have mainly focused on detecting D. nuttalli adult ticks (15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33) and D. nuttalli different developmental stages (34)(35)(36)(37)(38)(39)(40) carrying one specific pathogen or on next-generation sequencing of D. nuttalli adult ticks (41)(42)(43)(44). The microbial community structure and diversity of D. nuttalli at different developmental stages have not been comprehensively compared.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of microbial communities in different growth stages of Dermacentor nuttalli

Zhao

Ma²,

Yang³

et al. 2022

Front. Vet. Sci.

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Ticks were identified as arthropods that are pathogenic vectors. Dermacentor nuttalli is one of the dominant tick species in Inner Mongolia, and it carries and transmits a wide range of pathogenic microorganisms. However, at present, only the detection of D. nuttalli adult ticks and D. nuttalli different developmental stages carrying one specific pathogen, or the next-generation sequencing of D. nuttalli adult ticks were available. In this study, we investigated the microbial community structures of D. nuttalli in different growth stages under laboratory artificial feeding conditions. Total DNA was extracted from seven growth stages (female adult ticks, eggs, larval ticks, engorged larval ticks, nymphal ticks, engorged nymphal ticks, and second-generation adult ticks) obtained from laboratory artificial feeding of engorged D. nuttalli female ticks in Inner Mongolia. Then, the 16S rDNA V3–V4 hypervariable region was amplified to construct an Illumina PE250 library. Finally, 16S rRNA sequencing was performed on Illumina Novaseq 6000 platform. The sequencing data were analyzed using molecular biology software and platforms. The Illumina PE250 sequencing results showed that the egg stage had the highest diversity and number of species (28.74%, 98/341), while the engorged nymph stage had the lowest diversity and number of species (9.72%, 21/216). A total of 387 genera of 22 phyla were annotated in D. nuttalli, with 9 phyla and 57 genera found throughout all 7 growth stages. The dominant phylum was Proteobacteria; the dominant genera were Arsenophonus and Rickettsia; and the genera with the highest relative abundance in the 7 growth stages were Pseudomonas, Paenalcaligenes, Arsenophonus, Arsenophonus, Pseudomonas, Arsenophonus, and Rickettsia, respectively. Among the 23 exact species annotated, Brucella melitensis exhibits pathogeny that poses a serious threat to humans and animals. In this study, the microbial community composition at different growth stages of D. nuttalli was comprehensively analyzed for the first time.

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“…They transmit diseases through blood-sucking bites or contact with the host’s body fluids, blood, or animal products (Huang et al. 2020 ). The pathogens they carry can cause symptoms such as nausea, headache, fever, cytopenias (Wang et al.…”

Section: Introductionmentioning

confidence: 99%

The complete mitochondrial genome of Rhipicephalus haemaphysaloides and its phylogenetic analysis

Tang,

Zhang,

et al. 2024

Mitochondrial DNA Part B

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We conducted an analysis of the complete mitochondrial genome of Rhipicephalus haemaphysaloides , a tick species known for transmitting various bacteria and viruses. The mitochondrial genome of R. haemaphysaloides has a length of 14,739 bp and consists of 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes (rRNAs), and 2 control regions. By utilizing the maximum likelihood method, we established the phylogenetic relationship among R. haemaphysaloides and other species within the Rhipicephalus genus of the Ixodidae family. This analysis revealed that R. haemaphysaloides and other Rhipicephalus species belong to the same clade, further affirming the taxonomic placement of R. haemaphysaloides within the Rhipicephalus genus. Furthermore, we compared the mitochondrial genomes of R. haemaphysaloides isolates from Changning, Yunnan Province, China, with isolates from Yangxin, Ganzhou, and Yingtan, Hubei Province, China. In summary, our investigation offers genetic proof endorsing the taxonomic categorization and phylogenetic placement of Ixodidae by assessing the entire mitochondrial genome of R. haemaphysaloides .

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A Novel Arthropod Host of Brucellosis in the Arid Steppe Ecosystem

Cited by 13 publications

References 31 publications

Evidence-practice gap analysis in the role of tick in brucellosis transmission: a scoping review

Evidence-practice gap analysis in the role of tick in brucellosis transmission: a scoping review

Comparative analysis of microbial communities in different growth stages of Dermacentor nuttalli

The complete mitochondrial genome of Rhipicephalus haemaphysaloides and its phylogenetic analysis

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