A novel approach to prepare PA6/Fe₃O₄ microspheres for protein immobilization

Abstract

A novel method for preparation of polymerbased magnetic microspheres was proposed by utilizing melt reactive blending, which was based on selective location of Fe 3 O 4 nanoparticles in PA6 domain of polystyrene (PS)/polyamide 6 (PA6) immiscible blends. The results showed that most of Fe 3 O 4 was located in the PA6 microspheres. Magnetization data revealed the magnetite content of PA6/Fe 3 O 4 microsphere could be up to 54 wt % with strong magnetic responsibility and high saturation magnetization. Carboxyl functional group, bonded with PA6/ Fe 3 O 4 microsphere by copolymerization of acrylic acid with PA6 chain in different concentration ethanoic acid (HAc) solution, was used as a ligand for protein adsorption. The amount of adsorbed bovine serum albumin (BSA) was optimized by changing the medium pH and the initial concentrations of BSA. The results denoted that the adsorption capacity of BSA reaches 215 mg/g microspheres, showing potentials to promising applications in bioseparation and biomedical fields.

Nanostructure development in multicomponent polymer systems characterized by synchrotron X-ray scattering

“…In bioengineering, finely divided polyamide particles are attractive carriers for protein or enzyme immobilization with applications in solid-phase diagnostics, biosensors, biocatalysts, and bio-separation [23][24][25]. Polymer particles with magnetic or conductive loads may become interesting in applications requiring stimuli responsiveness [26,27]. Neat polyamide microparticles can be prepared by activated anionic ring-opening polymerization (AAP) of lactams in hydrocarbon solutions [19,20,[28][29][30].…”

One‐Step In Situ Synthesis of Polyamide Microcapsules With Inorganic Payload and Their Transformation into Responsive Thermoplastic Composite Materials

“…In bioengineering, finely divided porous polyamides are an attractive carrier for protein or enzyme immobilization and find applications in solid‐phase diagnostics, biosensors, biocatalysts, and bio‐separation . Polymer particles with magnetic or conductive loads are intensively studied in applications requiring stimuli responsiveness …”

“…[5][6][7] Polymer particles with magnetic or conductive loads are intensively studied in applications requiring stimuli responsiveness. [8,9] Powdered polyamide systems can be prepared by physical methods such as precipitation of polyamide solutions with non-solvents, [10][11][12] or by grinding after bulk homopolymerization. [13,14] Chemical approaches are also reported for the synthesis of powdered neat npolyamides such as polyamide 6 (PA6) and polyamide 12 (PA12) employing activated anionic ring-opening polymerization (AAP) of lactams in hydrocarbon solutions.…”

Modeling magneto-mechanical behavior of Fe3O4 nanoparticle/polyamide nanocomposite membrane in an external magnetic field

“…It was supposed that the nanoparticles with magnetic behaviors are in direct contact or mechanically connected to polymer matrix. 22,[42][43][44][45][46] There are two types of result from the presence of magnetic field with uniformity and a gradient magnetic field on nanoparticles including magnetic moments or torques and magnetic induced normal forces, respectively. In this research, for evaluating displacement field vectors inside the polyamide membrane, finite element method (FEM) is used as the analysis and simulation method of nanocomposite polymeric film.…”