A novel approach to identify molecular binding to the influenza virus H5N1: screening using molecularly imprinted polymers (MIPs)

Wangchareansak, Thipvaree; Thitithanyanont, Arunee; Chuakheaw, Daungmanee; Gleeson, M. Paul; Lieberzeit, Peter A.; Sangma, Chak

doi:10.1039/c3md00272a

Cited by 38 publications

(26 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Epitope-based MIPs targeted to bind the the glycoprotein 41 (gp41) transmembrane protein of HIV-1 were developed by Lu et al [87], where it was found that the MIPs had a dissociation constant for the template similar to that of monoclonal antibodies, as well has having a high specificity to the target. More recent research by Wangchareansak et al [88] involved the development of surface-imprinted polymers specific to the influenza H5N1 virus, and its analyte binding was tracked by a QCM sensor. There is a wide commercial interest for MIPs, particularly in solid phase extraction of samples and today there are few companies marketing these products such as Sigma-Aldrich's and POLYINTELL, France.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Biosensors for waterborne viruses: Detection and removal

et al. 2015

View full text Add to dashboard Cite

Section: Accepted Manuscriptmentioning

confidence: 99%

Biosensors for waterborne viruses: Detection and removal

et al. 2015

View full text Add to dashboard Cite

“…MIP‐based sensors can identify structures ranging from small to large molecules, proteins, or even whole microorganisms such as bacteria or viruses . In case of virus MIP biosensors, the main applications are virus detection, classification, or virus binding assays . However, applications of virus imprinting beyond these types have not been reported.…”

Section: Figurementioning

confidence: 99%

Small‐Molecule Dengue Virus Co‐imprinting and Its Application as an Electrochemical Sensor

et al. 2017

Self Cite

View full text Add to dashboard Cite

Polymers can be synthesized to recognize small molecules. This is achieved by introducing the target molecule during monomer self‐assembly, where they can be incorporated during cross‐linking polymerization. Following additional pre‐processing, the material obtained can then be applied as a sensing layer for these molecules in many applications. The sensitivity of the polymers depends on the “active sites” imprinted on the surface. Increasing the number of active sites on the polymers surface can be achieved by using nanoparticles as a platform to support and concentrate the molecules for imprinting. In this work, we report the first use of dengue virus as a supporting nanoparticle to make for a more effective polymer composite sensor for the detection of bisphenol A (BPA), which is an environmental contaminant. The dengue virus has a nanoparticle size of around 100 nm and its surface provides regions where lipids and hydrophobic compounds can bind, making it an ideal support. The mixing of BPA with dengue prior to monomer self‐assembly led to imprinted polymer surfaces with much higher density BPA binding sites and a limit of detection of 0.1 pm. We demonstrate that a BPA–dengue co‐imprinting polymer composite sensor shows a very high sensitivity for BPA, but with lower production costs and technical requirements than other comparable methods.

show abstract

“…The sensing measurements revealed that each type of imprinted surface offered the highest sensor response to its templated strain virus as shown in Figure 3B, thus proving excellent selectivity. Similarly, the surface imprinted polymers designed by soft-lithography procedure are successfully employed in QCM-based viral diagnostics for influenza A virus sub-types, i.e., H5N1, H5N3, H1N1, H1N3, and H6N1 [67,78]. The fabricated sensors demonstrated considerable selectivity for screening of influenza A virus sub-types as each sub-type was best recognized by its own imprinted surface.…”

Section: Synthetic Antibodies: Molecularly Imprinted Polymers For Qcmmentioning

confidence: 99%

“…The fabricated sensors demonstrated considerable selectivity for screening of influenza A virus sub-types as each sub-type was best recognized by its own imprinted surface. The reported detection limit was 10 5 virus particles/mL [67]. In view of these examples, it is obvious that soft-lithography approach to design surface MIPs makes them capable of selectively distinguishing different serotypes of the same virus, which may be termed as intra-group selectivity.…”

Section: Synthetic Antibodies: Molecularly Imprinted Polymers For Qcmmentioning

confidence: 99%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

View full text Add to dashboard Cite

Viruses are pathogenic microorganisms that can inhabit and replicate in human bodies causing a number of widespread infectious diseases such as influenza, gastroenteritis, hepatitis, meningitis, pneumonia, acquired immune deficiency syndrome (AIDS) etc. A majority of these viral diseases are contagious and can spread from infected to healthy human beings. The most important step in the treatment of these contagious diseases and to prevent their unwanted spread is to timely detect the disease-causing viruses. Gravimetric viral diagnostics based on quartz crystal microbalance (QCM) transducers and natural or synthetic receptors are miniaturized sensing platforms that can selectively recognize and quantify harmful virus species. Herein, a review of the label-free QCM virus sensors for clinical diagnostics and point of care (POC) applications is presented with major emphasis on the nature and performance of different receptors ranging from the natural or synthetic antibodies to selective macromolecular materials such as DNA and aptamers. A performance comparison of different receptors is provided and their limitations are discussed.

show abstract

A novel approach to identify molecular binding to the influenza virus H5N1: screening using molecularly imprinted polymers (MIPs)

Abstract: We investigate whether a molecularly imprinted polymer (MIP) of influenza A H5N1 could be used to help identify molecules capable of binding to, and inhibiting the function of the virus, via either competitive or allosteric mechanisms.

Cited by 38 publications

References 24 publications

Biosensors for waterborne viruses: Detection and removal

Biosensors for waterborne viruses: Detection and removal

Small‐Molecule Dengue Virus Co‐imprinting and Its Application as an Electrochemical Sensor

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

Contact Info

Product

Resources

About