2013
DOI: 10.7314/apjcp.2013.14.12.7523
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A Novel Approach to Cloning and Expression of Human Thymidylate Synthase

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Cited by 3 publications
(3 citation statements)
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“…In the same series we now assessed the expression of two other biomarkers with potential prognostic impact: the topoisomerase II alpha (TOP2A), an enzyme responsible for transcription, replication and chromosome condensation and segregation during cell division (Kellner et al 2002); and the thymidylate synthase (TS), an enzyme involved in nucleotide metabolism (Lv et al 2013). TOP2A is also a well-known predictor of efficacy of anthracyclines and topoisomerase inhibitors (Wang et al 2012) and TS is predictive of the efficacy of fluoropyrimidines (Formentini et al 2004).…”
Section: Introductionmentioning
confidence: 99%
“…In the same series we now assessed the expression of two other biomarkers with potential prognostic impact: the topoisomerase II alpha (TOP2A), an enzyme responsible for transcription, replication and chromosome condensation and segregation during cell division (Kellner et al 2002); and the thymidylate synthase (TS), an enzyme involved in nucleotide metabolism (Lv et al 2013). TOP2A is also a well-known predictor of efficacy of anthracyclines and topoisomerase inhibitors (Wang et al 2012) and TS is predictive of the efficacy of fluoropyrimidines (Formentini et al 2004).…”
Section: Introductionmentioning
confidence: 99%
“…Chemotherapy is an important therapeutic modality in the treatment of lung adenocarcinoma, however, the efficacy of the currently available therapy is limited ( 1 ). The enzyme, thymidylate synthase (TS), catalyzes the intracellular reaction that provides the sole de novo intracellular source of deoxythymidine monophosphate, or thymidylate, which is required for DNA replication and repair ( 4 , 5 ). The TS protein and messenger RNA levels are elevated in a number of human cancers ( 6 ) and have been found to correlate with poor prognosis in patients with colorectal ( 8 ), breast ( 9 ), head and neck ( 10 ) and pancreatic cancer.…”
Section: Introductionmentioning
confidence: 99%
“…Cells may predispose to carcinogenesis if genetic alterations occur in any critical regulatory pathway; the main critical regulatory pathways include regulations of xenobiotic metabolism, control of genomic stability, including deoxyribonucleic acid (DNA) repair mechanisms, cellcycle checkpoints, apoptosis, and control of growth and inflammatory factors (Wu et al, 2004). Thymidylate synthase is one of the enzymes involved in one carbon- Thymidylate synthase (ts) catalyzes the methylation of deoxyuridylate to deoxythymidylate utilizing 5,10methylene-THF as a cofactor (Longley et al, 2003;Lv et al, 2013). ts has three polymorphisms that may affect ts expression; two functionally important polymorphisms; one is a promoter-enhancer region (TSER), containing 2 or 3 tandem repeats of 28 bp units (2R or 3R) (rs34743033; NC_000018.10: 657645-657731) and known to play a role in the transcriptional autoregulation mechanism (Horie et al, 1995).…”
Section: Introductionmentioning
confidence: 99%