A Novel Approach of Synthesizing and Evaluating the Anticancer Potential of Silver Oxide Nanoparticles in vitro

Banerjee, Kaushik; Das, Sukta; Choudhury, Pritha; Ghosh, Sarbari; Baral, Rathindranath; Choudhuri, Soumitra Kumar

doi:10.1159/000453446

Cited by 34 publications

(13 citation statements)

References 31 publications

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“…The spherical bio-AgNPs with sizes ranging from 5-50 nm showed inhibitory activities against the lymphoma Jurkat cell line, and the IC 50 was 21.05 µg/mL [132]. The B16F10 melanoma cell line was inhibited by the 20-228 nm spherical bio-AgNPs, with an IC 50 value of 7.6 ± 0.8 µg/mL, following a dose-dependent relationship [101,133]. The 100-120 nm flower-shaped AgNPs exhibited notable repressive actions against the KB oral cancer cell line, where the IC 50 was 0.6 µg/mL [134].…”

Section: Biosynthesized Agnps As Anticancer Agents In Vitromentioning

confidence: 97%

Green Chemistry Synthesis of Silver Nanoparticles and Their Potential Anticancer Effects

Ratan

Haidere

Nurunnabi

et al. 2020

Cancers

207

108

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Nanobiotechnology has grown rapidly and become an integral part of modern disease diagnosis and treatment. Biosynthesized silver nanoparticles (AgNPs) are a class of eco-friendly, cost-effective and biocompatible agents that have attracted attention for their possible biomedical and bioengineering applications. Like many other inorganic and organic nanoparticles, such as AuNPs, iron oxide and quantum dots, AgNPs have also been widely studied as components of advanced anticancer agents in order to better manage cancer in the clinic. AgNPs are typically produced by the action of reducing reagents on silver ions. In addition to numerous laboratory-based methods for reduction of silver ions, living organisms and natural products can be effective and superior source for synthesis of AgNPs precursors. Currently, plants, bacteria and fungi can afford biogenic AgNPs precursors with diverse geometries and surface properties. In this review, we summarized the recent progress and achievements in biogenic AgNPs synthesis and their potential uses as anticancer agents.

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Section: Biosynthesized Agnps As Anticancer Agents In Vitromentioning

confidence: 97%

Green Chemistry Synthesis of Silver Nanoparticles and Their Potential Anticancer Effects

Ratan

Haidere

Nurunnabi

et al. 2020

Cancers

207

108

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“…Apoptosis induced by silver nanoparticles of different tumor cells has already been reported in vitro and ex vivo, which possibly occurred because of the translocation of the silver within the nucleus [83][84][85][86]. Franco-Molina et al reported that MCF-7 breast cancer cells treated with silver colloids exhibited considerably decreased dehydrogenase activity, causing NADH/NAD + reduction [87].…”

Section: Cytotoxicity By Brine Shrimp Lethality and Mtt Assay In Vitromentioning

confidence: 97%

Synthesis of Functional Silver Nanoparticles and Microparticles with Modifiers and Evaluation of Their Antimicrobial, Anticancer, and Antioxidant Activity

Dilshad

Bibi

Sheikh

et al. 2020

JFB

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An accumulating body of evidence reports the synthesis and biomedical applications of silver nanoparticles. However, the studies regarding the use of maleic acid and citric acid in the synthesis of nano-sized silver particles (AgNPs) and micro-sized silver particles (AgMPs) as well as their antibacterial, antifungal, and anticancer activities have not been reported. In the current study, we synthesized AgNPs and AgMPs using maleic acid and citric acid as capping agents and have characterized them by UV-Vis, energy-dispersive X-Ray spectroscopy (EDS), X-Ray diffraction (XRD), and scanning electron microscope (SEM) analysis. The capped silver particles were examined for their antimicrobial activity and cytotoxicity against bacteria, fungi, and brine shrimp. Additionally, the anticancer activity of these particles was tested against human breast and liver cancer cell lines. The free radical scavenging activity of capped silver particles was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. SEM analysis revealed a round plate-like morphology of maleic acid capped particles with an average size of 39 ± 4 nm, whereas citric acid capped particles display flower-shaped morphology with rough surfaces and an average size of 250 ± 5 nm. The uncapped AgMPs were hexagonal with 500 ± 4 nm size. EDS and XRD analysis confirmed the presence of Ag and face-centered cubic crystalline nature, respectively. Functionally, capped silver particles exhibited antibacterial activity against Gram-positive (Staphylococcus aureus, Bacillus subtilis, and Micrococcus luteus) and Gram-negative bacteria (Salmonella setubal, Enterobacter aerogenes, and Agrobacterium tumefaciens). The bactericidal activity was more active against Gram-negative bacteria with minimum inhibitory concentration (MIC) as low as 5 ppm as compared to 25 ppm for Gram-positive. Similarly, the silver particles demonstrated antifungal activity by inhibiting the growth of five fungal strains (Mucor species, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, and Fusarium solani) up to 50% at the concentration of 500 ppm. Additionally, these particles showed substantial toxicity against brine shrimp and also significantly inhibited the proliferation of breast cancer (MCF7) and liver cancer (HePG2) cell lines (IC50 8.9–18.56 µM). Uncapped AgMPs were less effective, inhibiting only the proliferation of MCF7 cells with IC50 46.54 µM. Besides cytotoxicity, these particles acted as potential antioxidants, showing free radical scavenging up to 74.4% in a concentration-dependent manner. Taken together, our results showed that the modifiers affect the shape and size of silver particles and may, in part, contribute to the antimicrobial and antioxidant activity of silver particles. However, the contribution of maleic acid and citric acid in enhancing the antimicrobial, anticancer, and antioxidant potential independent of silver nano and microparticles needs to be studied further. In vivo experiments may determine the therapeutic effectiveness of silver particles capped with these modifiers.

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“…These NPs effectively inhibited cell proliferation, angiogenesis, colony formation, and spheroid formation of breast cancer cells through apoptosis induction; HIF-1α, VEGF, Cyclin D1, and STAT-3 gene down-regulation; and Caspase-9 gene up-regulation. Ag-oxide NPs synthesized utilizing an aqueous leaf extract from Excoecaria agallocha , and with anticancer potential, were evaluated by Banerjee et al [ 139 ]. These Ag-based NPs were spherical shaped and exhibited an average particle size of 228 nm.…”

Section: Cancer Evaluation Modelsmentioning

confidence: 99%

“…These Ag-based NPs were spherical shaped and exhibited an average particle size of 228 nm. They induced a growth-inhibitory effect against murine Ehrlich ascites carcinoma cells, with an IC 50 value of 1.1 ± 0.1 µg/mL at 72 h. Their cytotoxic effect was mediated via apoptosis induction as the number of annexin V-positive cells increased as a function of time [ 139 ].…”

Section: Cancer Evaluation Modelsmentioning

confidence: 99%

Green Metallic Nanoparticles for Cancer Therapy: Evaluation Models and Cancer Applications

et al. 2021

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Metal-based nanoparticles are widely used to deliver bioactive molecules and drugs to improve cancer therapy. Several research works have highlighted the synthesis of gold and silver nanoparticles by green chemistry, using biological entities to minimize the use of solvents and control their physicochemical and biological properties. Recent advances in evaluating the anticancer effect of green biogenic Au and Ag nanoparticles are mainly focused on the use of conventional 2D cell culture and in vivo murine models that allow determination of the half-maximal inhibitory concentration, a critical parameter to move forward clinical trials. However, the interaction between nanoparticles and the tumor microenvironment is not yet fully understood. Therefore, it is necessary to develop more human-like evaluation models or to improve the existing ones for a better understanding of the molecular bases of cancer. This review provides recent advances in biosynthesized Au and Ag nanoparticles for seven of the most common and relevant cancers and their biological assessment. In addition, it provides a general idea of the in silico, in vitro, ex vivo, and in vivo models used for the anticancer evaluation of green biogenic metal-based nanoparticles.

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A Novel Approach of Synthesizing and Evaluating the Anticancer Potential of Silver Oxide Nanoparticles in vitro

Cited by 34 publications

References 31 publications

Green Chemistry Synthesis of Silver Nanoparticles and Their Potential Anticancer Effects

Green Chemistry Synthesis of Silver Nanoparticles and Their Potential Anticancer Effects

Synthesis of Functional Silver Nanoparticles and Microparticles with Modifiers and Evaluation of Their Antimicrobial, Anticancer, and Antioxidant Activity

Green Metallic Nanoparticles for Cancer Therapy: Evaluation Models and Cancer Applications

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