A Novel Approach for Studying Histone H1 Function <i>in Vivo</i>

Siriaco, Giorgia; Deuring, Renate; Mawla, Gina D.; Tamkun, John W.

doi:10.1534/genetics.114.170514

Cited by 2 publications

(1 citation statement)

References 33 publications

(43 reference statements)

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“…Instead, prior functional studies of H1 relies on RNAi knock-down or overexpression approaches. It has been shown that H1 is important for the maintenance of heterochromatin stability and gene expression (Ni et al 2006;Lu et al 2009Lu et al , 2013Vujatovic et al 2012), the S e p t e m b e r 4 , 2 0 1 9 5 regulation of DNA replication in polyploid cells (Andreyeva et al 2017), the compaction of chromatin in endo-replicating cells (Corona et al 2007;Lu et al 2009;Siriaco et al 2015), and stem cell maintenance in testis (Sun et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Compensatory replacement of the BigH1 variant histone by canonical H1 supports normal embryonic development in Drosophila

Han

Chen

et al. 2019

Preprint

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Histone variants carry specific functions in addition to those fulfilled by their canonical counterparts. Variants of the linker Histone H1 are prevalent in vertebrates and based on the pattern of their expression, many are presumed to function during germline and the earliest zygotic stages of development.While the existence of multiple H1 variants has hampered their study in vertebrates, a single variant, BigH1, was identified in Drosophila, promising to accelerate our understanding of the biological functions of H1 and H1 variants.Here we uncovered evidence for a compensatory activity that loads maternal H1 onto BigH1-devoid chromatin. Remarkably, this H1-based chromatin state is fully functional in supporting normal embryonic development, suggesting that H1 carries the essential function of the BigH1 molecule under the same developmental context. In addition, we discovered that this compensatory replacement of BigH1 with H1 might be limited to rapidly cycling cells in early embryos.

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