A Novel Approach for Skin Regeneration by a Potent Bioactive Placental-Loaded Microneedle Patch: Comparative Study of Deer, Goat, and Porcine Placentas

Abstract

The aims of this study were to investigate the skin regeneration potential of bioactive placenta (deer placenta (DP), goat placenta (GP), and porcine placenta (PP)) and fabricate bioactive extract-loaded dissolving microneedles (DMNs) as a dermal delivery approach. The placentas were water-extracted, and the active compounds were evaluated. Bioactivity studies were performed in dermal fibroblasts and keratinocytes. DMNs were fabricated to deliver the potent bioactive placenta extract into the skin. All placental extracts expressed high amounts of protein, growth factors (EGF, FGF, IGF-1 and TGF-β1), and amino acids. These extracts were not toxic to the skin cells, while the proliferation of fibroblast cells significantly increased in a time-dependent manner. GP extract that exhibited the maximum proliferation, migration, and regeneration effect on fibroblast cells was loaded into DMN patch. The suitable physical properties of DMNs led to increased skin permeation and deposition of bioactive macromolecules. Moreover, GP extract-loaded DMNs showed minimal invasiveness to the skin and were safe for application to human skin. In conclusion, placental extracts act as potent bioactive compounds for skin cells, and the highest bioactive potential of GP-loaded DMNs might be a novel approach to regenerate the skin.

Analysis of research trends on microneedle patches loaded pharmacopuncture preparation: A Literature Review

“…총 7개의 연구 중에서 3개는 류마티스 관절염 24,27) 또는 통풍 28) 과 같은 관절 질환을 대상으로 하였다. 3 개의 연구는 건선 23) , 비후성 흉터 26) , 세포 재생 29) 과 같은 피부과 질환을 대상으로 하였다. 나머지 1개의 연구는 신경변성질환 25) 을 타겟으로 하였다(Table 1 and 2).…”

“…마이크로니들 패치의 적용 시간은 2건 25,29) 은 4일 이내(1일, 4일), 3건 24,26,27) 은 2주~6주 사이로 지속하 였다. 2건 23,28) 은 압력과 함께 적용하여 3분 동안 유 지하였다(Table 1 and 2).…”

Microneedles for in situ tissue regeneration

“…Here, MNs with micro-needles structure provide an effective solution without using syringes which may cause inflammation and pain. For instance, by means of simple finger pressure, MNs can penetrate the skin tissue and deliver the active substance to the wound bed [ 71 ]. And MNs can effectively encapsulate cells or bioactive substances and maintain their good activity.…”