A Novel Approach for Nucleic Acid Delivery Into Cancer Cells

Abstract

Background. Liposomal magnetofection is based on the use of superparamagnetic particles and cationic lipids and shows better transfection efficiency than other common nonviral gene delivery methods; however, the distribution of aggregate complexes over the cell surface may be ununiform. The use of a dynamic gradient magnetic field could overcome this limitation. A newly developed device for magnetofection under a dynamic magnetic field was used to compare the transfection efficiency of prostate carcinoma cell line PC3 with that obtained by lipofection and magnetofection. Material and Methods. Reporter plasmid pcDNA3.1LacZ DNA was used in combination with Lipofectamine2000 reagent and superparamagnetic nanoparticles CombiMag. The effects of incubation time under a dynamic magnetic field and a rotation frequency of magnets on transfection efficiency for PC3 cell line were determined. Alternatively, lipofection and liposomal magnetofection were carried out. Transfection efficiency of delivery methods was estimated by β-galactosidase staining; cell viability, by acridine orange/ethidium bromide staining. Results. Liposomal magnetofection under a dynamic gradient magnetic field demonstrated the highest transfection efficiency: it was greater by almost 21% and 42% in comparison with liposomal magnetofection and lipofection, respectively. The optimal incubation time under dynamic magnetic field and the optimal magnet rotation frequency were 5 minutes and 5 rpm, respectively. Liposomal magnetofection under a dynamic gradient magnetic field was less cytotoxic (7%) than that under a permanent magnetic field (17%) and lipofection (11%). Conclusions. Our new approach, based on the use of a dynamic gradient magnetic field, enhanced the transfection efficiency and had a less cytotoxic effect on prostate cancer cells in comparison with the standard magnetofection and lipofection.

Biodistribution, biocompatibility and targeted accumulation of magnetic nanoporous silica nanoparticles as drug carrier in orthopedics

“…Magnetic nanoparticles are already versatilely used in research and partly in clinical issues for hyperthermia or drug delivery in tumor [31][32][33][34][35] and infection treatment [36,37], as contrast agents for magnetic resonance imaging [38][39][40], and others [41,42]. The biocompatibility of certain magnetic nanoparticles with different composition, magnetic properties or size has already been published [43,44].…”

The Effect of Cyclic Movement of Magnets on the Sedimentation of Magnetic Nanoparticles in Magnetofection Devices: Computer Simulation

“…It has been reported by [6] that the efficiency of magnetofection increases A c c e p t e d M a n u s c r i p t 4 results obtained in this work allow to suggest that the dynamics of nanoparticles is determined basically by the balance of magnetic and viscous forces, but random forces, hydrodynamic interactions, etc. give an insignificant modulation of the trajectory, which we ignore in this work.…”

Magnetic micelles for DNA delivery to rat brains after mild traumatic brain injury

“…In absence of a magnetic field these particles show less aggregation which prevents their accumulation in the blood vessels [10]. Since its discovery, the technique of magnetofection has been primarily used to increase the gene transfection of the in vitro cell cultures using the superparamagnetic nanoparticles [15, 24, 25]. The gradient of the magnetic field enhances the efficiency of magnetofection compared to that without magnetic field [10, 26].…”