A novel approach for investigation of specific and cross-reactive IgE epitopes on Bet v 1 and homologous food allergens in individual patients

Structure and Function of the Peanut Panallergen Ara h 8

“…This region corresponds to the so-called "P-loop," the highly conserved region that is characteristic of the allergenic PR-10 proteins (37). This region was already shown to be involved in cross-reactivity between Bet v 1 and its homologs (49,50 , and Ala 152 , which are conserved in both Ara h 8 and Bet v 1, form a relatively large, solvent-exposed patch on the surface of these allergens. Some of these residues were identified as part of the IgE epitope, with Glu 147 (Ara h 8 numbering) being the most critical (53).…”

Allergenicity and antigenicity of wild-type and mutant, monomeric, and dimeric carrot major allergen Dau c 1: Destruction of conformation, not oligomerization, is the roadmap to save allergen vaccines

“…However, this hypothesis may be tested by comparing the epitopes recognized the murine sera raised against native and misfolded Dau c 1 as well as sera from patients who underwent (successful) immunotherapy. A suitable approach to compare in detail the epitopes recognized by our mouse sera with those of IgE from subjects with carrot allergy could be the use of phage-displayed peptide mimics, as recently described by Mittag et al 26 Another question is whether monomeric or oligomeric allergen is the better reagent for immunotherapy. On the basis of the results, the dimeric (mutant) Dau c 1 molecule should be preferred because dimerization had an enhancing effect on the immunogenicity, indicated by the fact that the lowest dose of the dimer produced a clearly higher antibody response compared with the identical dose of the mixture of monomers.…”

Molecular basis of allergen cross-reactivity: Non-specific lipid transfer proteins from wheat flour and peach fruit as models

“…This selection was carried out as previously described (Mittag et al, 2006). A 2-mL aliquot of a suspension of Tosyl-activated M280 Dynabeads (Invitrogen Dynal, Oslo, Norway) was loaded with 100 (xg monoclonal mouse-anti-human IgE (ABR Affinity BioReagents) overnight.…”