A novel approach for direct shoot regeneration, anatomical characterization, and withanolides content in micropropagated plants of Withania somnifera (L.) Dunal—an important medicinal plant

Mahendran, Ganesan; Rahman, Laiq ur

doi:10.1007/s11627-024-10428-x

Cited by 2 publications

(2 citation statements)

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“…In contrast, the hormone-free medium was helpful in the shoot elongation from adventitious shoot buds initiated by various explants of eggplant(Mukhadir et al, 2016;Yesmin et al, 2018;, and media with the same concentrations and combinations were used for initiation of shoots(Bhat et al, 2021). Withania somnifera(Mahendran and Rahman, 2024), and Solanum lycopersicum(Marapaka et al, 2024).The elongated shoots obtained from different types of shoot induction media, such as mT and BAP, were evaluated for rooting e ciency. The rooting media are forti ed with diverse auxin concentrations individually, such as IAA (1.43 to 5.71 µM), IBA (1.23 to 4.92 µM), and NAA (1.34 to 5.37 µM), used to determine the rooting frequency and suitable concentration of auxin and production of complete plantlets.…”

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confidence: 99%

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Meta-Topolin induced highly efficient plant regeneration from various explants of eggplant (Solanum melongena L.)

Gande,

Marapaka,

Jogam

et al. 2024

Preprint

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Eggplant (Solanum melongena L.) is one of the essential vegetables worldwide, and cultivated genotypes of eggplant suffer from numerous abiotic and biotic stresses. A reproducible and efficient plant regeneration system is crucial for applying molecular breeding methods to overcome the difficulties of conventional breeding programs to improve eggplant germplasm, such as genetic transformation and genome editing techniques. The three explant types, cotyledon, hypocotyl, and leaf, were obtained from two different cultivars, Pusa purple long (PPL) and Pusa green red (PGR) of eggplant. Three explants were cultivated on media augmented with a variety of cytokinins like BAP, mT, and ZEA with different concentrations. The media supplemented with mT at 8.28 µM generated the highest number of shoots, which showed the optimum regeneration efficiency for all three explants in two eggplant genotypes. The cotyledon explants generated the optimum number of shoot buds on the medium amended with low concentrations of BAP (2.22 µM), KIN (2.32 µM), and ZEA (2.28 µM), and mT at 8.28 µM. The mT (8.28 µM) and BAP at 2.22 µM combinations produced 25.8 and 18.3 shoots in PPL and PGR genotypes, respectively. The addition of various concentrations of IAA (1.43 to 5.71 µM), IBA (1.23 to 4.92 µM), and NAA (1.34 to 5.37 µM) in combination with mT (8.28 µM) were evaluated to find out their role on the induction and proliferation of numerous shoot buds from cotyledon explants of two cultivars of eggplant. The medium augmented with mT (8.28 µM) and IAA (2.85 µM) produced 26.4 shoots and 17.8 shoots in cotyledon explants of PPL and PGR cultivars, respectively. The optimum rooting efficiency of shoots was recorded on the medium containing the IAA (5.71 µM) and produced complete plantlets. The plantlets showed 100% similarity with their mother plants.

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confidence: 99%

“…The shoots obtained from medium supplemented with mT show enhanced rooting e ciency compared to those generated from BAP-forti ed medium. It was observed that the mT improves the rooting of shoots obtained from mT-supplemented media(Khanam et al, 2020;Marapaka et al, 2024;Mahendran and Rahman, 2024).…”

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confidence: 99%