A novel approach for detection of brucella using a real-time recombinase polymerase amplification assay

Qin, Lide; Nan, Wenlong; Wang, Yong; Zhang, Yueyong; Tan, Pengfei; Chen, Yuqi; Mao, Kairong

doi:10.1016/j.mcp.2019.101451

Cited by 9 publications

(4 citation statements)

References 33 publications

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“…A dual PCR was established for distinguishing the B. suis vaccine strain S2 and B. suis biovar 1 among 76 field isolates representing all the common Brucella species and biovars, as well as four Brucella vaccine strains (104M, A19, S19, and M5) [89]. Qin et al developed a real-time recombinase polymerase amplification assay for the detection of Brucella, suggesting that this method could successfully distinguish 18 representative Brucella strains and four Brucella vaccine strains, including S2, A19, S19, and M5 [90]. Based on the genome-wide core-SNP typing platform, the B. suis vaccine strain S2 was discriminated from 54 B. suis strains with the accumulation of the highest number of SNPs [30,91] and using MLVA, Jiang et al identified the B. suis vaccine strain S2 isolate from cattle in China [23].…”

Section: Surveillance Of S2 Vaccine Immunizationmentioning

confidence: 99%

“…Qin et al. developed a real‐time recombinase polymerase amplification assay for the detection of Brucella , suggesting that this method could successfully distinguish 18 representative Brucella strains and four Brucella vaccine strains, including S2, A19, S19, and M5 [90]. Based on the genome‐wide core‐SNP typing platform, the B. suis vaccine strain S2 was discriminated from 54 B .…”

Section: Surveillance Of S2 Vaccine Immunizationmentioning

confidence: 99%

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The advances of the Chinese Brucella suis strain 2 vaccine

Jiang

Feng

et al. 2023

Animal Research and One Health

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Brucellosis, a worldwide crucial zoonotic disease, poses global socioeconomic risks to the system by threatening human health and animal reproduction. In recent years, with the increase of animal husbandry, animal brucellosis has become a remarkable health issue in China. To prevent and control the spread of brucellosis, Brucella suis live strain S2 vaccine has been licensed as a multivalent vaccine against the three major zoonotic Brucella species, for example, Brucella melitensis (small ruminants), Brucella abortus (bovids), and B. suis (swine). Following this process, the country enforced control measures based upon B. suis S2 vaccination, which reduced the disease burden in 2022 to 0.66% in the livestock population. The aim of this paper is to review and discuss the biological properties of the S2 vaccine and its wide use in vaccination programs of China, highlighting oral vaccination advantages, validation, and standardization of this vaccine as well as the progress of the S2 vaccination program achieved in the national control of the three zoonotic Brucella species.

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Section: Surveillance Of S2 Vaccine Immunizationmentioning

confidence: 99%

Section: Surveillance Of S2 Vaccine Immunizationmentioning

confidence: 99%

The advances of the Chinese Brucella suis strain 2 vaccine

Jiang

Feng

et al. 2023

Animal Research and One Health

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“…Detection of the H9-subtype of Avian influenza virus by RPA LFD assay with 10 × more sensitivity than conventional RT-PCR has also been documented (Wang et al 2019). Several other significant animal diseases for which RPA-LFD assays have been developed include detection of Porcine circovirus type 2 (PCV-2) (Yang et al 2017b), PPRV (Yang et al 2017c), ASFV (Miao et al 2019), Porcine deltacoronavirus (PDCoV) (Gao et al 2020), Pasteurella multocida (Zhao et al 2019), Bovine viral diarrhoea virus (BVDV) (Hou et al 2018a), Bovine ephemeral fever virus (BEFV) (Hou et al 2018b), Mycoplasma bovis (Zhao et al 2018), Infectious bovine rhinotracheitis virus (IBRV) (Hou et al 2017), FMDV (Wang et al 2018b), Brucella species (Qin et al 2019), PRRSV (Wang et al 2017) and LSDV (Shalaby et al 2016). The use of RPA-LFD assay in diagnostic application is therefore becoming a molecular tool of choice for the rapid, specific, and cost-effective approach for identification of animal diseases in laboratory settings as well as in field conditions.…”

Section: Nucleic Acid-based Detection Systemsmentioning

confidence: 99%

Point of care diagnostics and non-invasive sampling strategy: a review on major advances in veterinary diagnostics

et al. 2022

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The use of point of care diagnostics (POCD) in animal diseases has steadily increased over the years since its introduction. Its potential application to diagnose infectious diseases in remote and resource limited settings have made it an ideal diagnostic in animal disease diagnosis and surveillance. The rapid increase in incidence of emerging infectious diseases requires urgent attention where POCD could be indispensable tools for immediate detection and early warning of a potential pathogen. The advantages of being rapid, easily affordable and the ability to diagnose an infectious disease on spot has driven an intense effort to refine and build on the existing technologies to generate advanced POCD with incremental improvements in analytical performance to diagnose a broad spectrum of animal diseases. The rural communities in developing countries are invariably affected by the burden of infectious animal diseases due to limited access to diagnostics and animal health personnel. Besides, the alarming trend of emerging and transboundary diseases with pathogen spill-overs at livestock-wildlife interfaces has been identified as a threat to the domestic population and wildlife conservation. Under such circumstances, POCD coupled with non-invasive sampling techniques could be successfully deployed at field level without the use of sophisticated laboratory infrastructures. This review illustrates the current and prospective POCD for existing and emerging animal diseases, the status of non-invasive sampling strategies for animal diseases, and the tremendous potential of POCD to uplift the status of global animal health care.

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“…A total of 52 Brucella field strains were detected by real-time PCR and RPA in parallel, and compared with real-time PCR, the sensitivity of the RPA assay was 94%. Thus, this RPA assay may be a rapid, sensitive, and specific tool for the prevention and control of Brucellosis [80].…”

Section: Diagnosis Of Brucellosismentioning

confidence: 99%

Polymorphism Of Osteoarticular Manifestation Of Brucellosis Infection. A Review

Yu.

Masoodi²,

Kurmanova

et al. 2020

IAM

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Brucellosis is a common zoonosis which still remains as a major health problem in certain parts of the world. Kazakhstan remains among the most disadvantaged territories of brucellosis from Commonwealth of Independent States countries. The involvement of the musculoskeletal system is one of the most common systemic manifestations in brucellosis infection. The frequency of osteoarticular involvement of brucellosis varies between 10% and 85%. Osteoarticular involvement includes spondylitis, sacroiliitis, osteomyelitis, peripheral arthritis, bursitis, and tenosynovitis. Sacroiliitis is the most common osteoarticular finding in adults. A high degree of suspicion in the diagnosis of brucellar spondylitis is essential to reduce the delay for the treatment. Thus, it should be essentially included in the differential diagnosis of longstanding back pain particularly in regions where brucellosis is endemic. Screening serologic tests for brucella should be used more widely even in presence of low index of suspicion, especially in endemic areas. According to studies, when diagnosed with chronic brucellosis, the results of serological studies were unreliable: the result of the standard agglutination test (SAT)-Wright's reaction was negative in 32.7% of cases in patients with chronic brucellosis. Imaging studies, including radiography, computed tomography (CT), magnetic resonance (MR) imaging, and bone scintigraphy, have been used for diagnosis. Radiography is limited to evaluating the focal form of spinal brucellosis and advanced disease at the joints. For instance, MR imaging has a low specificity to predict the exact cause of an osteoarticular lesion, and in case of arthralgia or symptoms of osteomyelitis or spondylodiscitis, the index of suspicion should be high in regions where the disease is endemic.

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A novel approach for detection of brucella using a real-time recombinase polymerase amplification assay

Cited by 9 publications

References 33 publications

The advances of the Chinese Brucella suis strain 2 vaccine

The advances of the Chinese Brucella suis strain 2 vaccine

Point of care diagnostics and non-invasive sampling strategy: a review on major advances in veterinary diagnostics

Polymorphism Of Osteoarticular Manifestation Of Brucellosis Infection. A Review

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