A novel approach for 3D reconstruction of mice full-grown oocytes by time-of-flight secondary ion mass spectrometry

Гулин, А. А.; Надточенко, В. А.; Solodina, Alyona; Pogorelova, M. A.; Panait, A. I.; Pogorelov, A. G.

doi:10.1007/s00216-019-02237-8

Cited by 8 publications

(8 citation statements)

References 54 publications

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“…4,[29][30][31] Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a surface sensitive technique that allows the detection and 3D visualization of organic compounds in cells with submicron spatial resolution. [32][33][34] ToF-SIMS is mainly oriented on the analysis of lipids and small metabolites due to the high energy of primary ions. High chemical specificity and sensitivity make ToF-SIMS a valuable tool to detect unlabeled lipid species within plasma membrane.…”

Section: Introductionmentioning

confidence: 99%

Mapping cisplatin-induced viscosity alterations in cancer cells using molecular rotor and fluorescence lifetime imaging microscopy

et al. 2020

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. Significance: Despite the importance of the cell membrane in regulation of drug activity, the influence of drug treatments on its physical properties is still poorly understood. The combination of fluorescence lifetime imaging microscopy (FLIM) with specific viscosity-sensitive fluorescent molecular rotors allows the quantification of membrane viscosity with high spatiotemporal resolution, down to the individual cell organelles. Aim: The aim of our work was to analyze microviscosity of the plasma membrane of living cancer cells during chemotherapy with cisplatin using FLIM and correlate the observed changes with lipid composition and cell’s response to treatment. Approach: FLIM together with viscosity-sensitive boron dipyrromethene-based fluorescent molecular rotor was used to map the fluidity of the cell’s membrane. Chemical analysis of membrane lipid composition was performed with time-of-flight secondary ion mass spectrometry (ToF-SIMS). Results: We detected a significant steady increase in membrane viscosity in viable cancer cells, both in cell monolayers and tumor spheroids, upon prolonged treatment with cisplatin, as well as in cisplatin-adapted cell line. ToF-SIMS revealed correlative changes in lipid profile of cisplatin-treated cells. Conclusions: These results suggest an involvement of membrane viscosity in the cell adaptation to the drug and in the acquisition of drug resistance.

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Section: Introductionmentioning

confidence: 99%

Mapping cisplatin-induced viscosity alterations in cancer cells using molecular rotor and fluorescence lifetime imaging microscopy

et al. 2020

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“…The details of the TD‐ESI/MS setup has been previously described in the authors' previous publications 2–5 . The sample (either solid or liquid) was collected by a sampling probe, with the probe having a handle with a stainless steel inoculating loop and a circle at its tip (radius 2 mm).…”

Section: Methodsmentioning

confidence: 99%

“…The details of the TD-ESI/MS setup has been previously described in the authors' previous publications. [2][3][4][5] The sample (either solid or liquid) was collected by a sampling probe, with the probe having a handle with a stainless steel inoculating loop and a circle at its tip (radius 2 mm). Before sampling, a butane gas torch was used to heat the probe tip until the tip turns red to remove organic residues (Figure 1A).…”

Section: Td-esi/ms/ms Analysismentioning

confidence: 99%

“…Mass spectrometry imaging (MSI) is a technique that allows nonspecific detection of the spatial distribution of molecular species on a highly complex biological sample without labeling. [1][2][3][4][5] The molecular information obtained from mass spectrometry and visualized in a thin tissue section makes MSI a valuable analytical technique to ascertain the distribution of thousands of biomolecules such as metabolites, lipids, peptides, and proteins in samples. MSI's two most commonly used ionization methods are matrix-assisted laser desorption/ionization (MALDI) and secondary ionization mass spectrometry (SIMS).…”

Section: Introductionmentioning

confidence: 99%

“…In contrast, ambient ionization mass spectrometry (AIMS) efficiently detects organic and biological molecules under ambient conditions and without sample pretreatment. Several AIMS methods have been used for MSI analysis, such as desorption electrospray ionization (DESI), 10–13 electrospray laser desorption ionization (ELDI), 1,2 probe electrospray ionization (PESI), 3 laser ablation electrospray ionization (LAESI), 4,5 and low‐temperature plasma probe (LTP) 14 . The differences in MSI analysis between AIMS and MALDI include (1) minimal or no sample pretreatment required for AIMS‐MSI, with homogenous matrix deposition on tissue samples being necessary for MALDI‐MSI, and (2) desorption and ionization in AIMS being performed under ambient conditions, unlike for MALDI, where desorption/ionization is performed in a vacuum.…”

Section: Introductionmentioning

confidence: 99%

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Obtaining molecular imagings of pesticide residues on strawberry surfaces with probe sampling followed by ambient ionization mass spectrometric analysis

Jeng

Jiang

Cho³

et al. 2020

J Mass Spectrom

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Thermal desorption-electrospray ionization tandem mass spectrometry (TD-ESI/MS/MS) was used to rapidly characterize the residual pesticides collected on the surface of a strawberry with a metallic probe. Twelve pesticides, including nine fungicides and three miticides, were detected; the results were validated by comparison with results that used solvent extraction followed by gas chromatography/mass spectrometry and liquid chromatography/tandem mass spectrometry analyses. The distribution of pesticide residues on a strawberry's surface was explored by collecting multiple samples using probes from 40 positions on the strawberry, with the collected samples being analyzed with TD-ESI/MS/MS. The obtained molecular information was used to construct mass spectrometry imaging of the strawberry's pesticide residues.

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Surface analysis and interface properties of 2-aminobenzimidazole corrosion inhibitor for brass in chloride solution

Finšgar

2020

Anal Bioanal Chem

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A novel approach for 3D reconstruction of mice full-grown oocytes by time-of-flight secondary ion mass spectrometry

Cited by 8 publications

References 54 publications

Mapping cisplatin-induced viscosity alterations in cancer cells using molecular rotor and fluorescence lifetime imaging microscopy

Mapping cisplatin-induced viscosity alterations in cancer cells using molecular rotor and fluorescence lifetime imaging microscopy

Obtaining molecular imagings of pesticide residues on strawberry surfaces with probe sampling followed by ambient ionization mass spectrometric analysis

Surface analysis and interface properties of 2-aminobenzimidazole corrosion inhibitor for brass in chloride solution

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