A novel application of nanohydroxyapatite/mesoporous silica biocomposite on treating dentin hypersensitivity: An in vitro study

Yu, Jianming; Yang, Hongye; Li, Kang; Lei, Jian; Zhou, Liqun; Huang, Cui

doi:10.1016/j.jdent.2016.04.005

Cited by 62 publications

(72 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In accordance with the theory (Saito, Arsenault, Yamauchi, Kuboki, & Crenshaw, 1997;Saito, Yamauchi, Abiko, Matsuda, & Crenshaw, 2000) that demineralized collagen matrix do not have the ability to induce nucleation with the mineral source mainly calcium and ions from artificial saliva, in the present study when the SEM images were evaluated it has been detected that all tubules of the specimens in the control group were found to be open while in all the groups treated with nHAp and its fluoride doped types an effective tubule occlusion was achieved. These findings were parallel with the results stated in the similar studies which used commercial HAp including products (Bakry, Al-Hadeethi, & Razvi, 2016;Kulal et al, 2016;Lin et al, 2017;Wang et al, 2014;Yu et al, 2016;Yuan et al, 2012).…”

Section: Discussion Ssupporting

confidence: 88%

“…One of these strategies is to occlude the exposed dentin surface, especially the tubule orifices, by using various agents that includes different active ingredients (Arnold et al, ; Earl et al, ; Tian et al, ). Since hydroxyapatite is one of the main components of human bones and teeth and it has good biocompatibility and bioactivity, synthetic hydroxyapatite has become one of the hot topics for biological material research over the past few decades (Cummins, ; Lee et al, ; Lin et al, ; Wang et al, ; Yu et al, ). It is imperative that in vitro experiments be performed before a newly developed material is used in humans.…”

Section: Discussionmentioning

confidence: 99%

“…Hence, nHAp is very desirable as a natural occluding material (Lin et al, ). Due to its mineral components being similar to bone and teeth of human body nHAp has received much attention in orthopedics, plastic surgery, and dental treatmentin recent years (Liu, Huang, Shen, & Cui, ).In developing new desensitizing agents, there is an increasing demand on using various nanoparticles (Chiang et al, ; Lee et al, ; Wang et al, ) or their combinations (Liu et al, ; Lin et al, ) because of the possibility of these materials may easily penetrate into dentin tubules and also may react as mineralising agents (Kulal, Jayanti, Sambashivaiah, & Bilchodmath, ; Yu et al, ). In this way, it may be ensured to do effective and long‐lasting therapy because the materials penetrated into dentin tubules and/or made chemical reaction with tooth structure are likely to be resistant to mechanical and chemical factors (Lavender, ).…”

Section: Introductionmentioning

confidence: 99%

“…In this way, it may be ensured to do effective and long‐lasting therapy because the materials penetrated into dentin tubules and/or made chemical reaction with tooth structure are likely to be resistant to mechanical and chemical factors (Lavender, ). There are studies which demonstrated the dentinal tubule occluding (Chiang et al, ; Lavender, ; Lee et al, ; Wang et al, ) and even plugging ability of nHAp alone (Liu et al, ; Lin et al, ) or varius combinations with other molecules like E8DS peptide, mesoporous silica biocomposite, and functional dendrimers (Kulal et al, ; Yu et al, ). In these studies, it is stated that nHAp was found to be better than desensitizing agents containing fluoride or arginine by its property of surface remineralisation and formation of biomimetic apatite coating on the dental hard tissue surfaces.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Dentinal tubule occluding capability of nano‐hydroxyapatite; The in‐vitro evaluation

Bağlar

Erdem

Doğan

et al. 2018

Microscopy Res & Technique

View full text Add to dashboard Cite

In this in-vitro study, the effectiveness of experimental pure nano-hydroxyapatite (nHAP) and 1%, 2%, and 3% F¯ doped nano-HAp on dentine tubule occlusion was investigated. And also, the cytotoxicity of materials used in the experiment was evaluated. Nano-HAp types were synthesized by the precipitation method. Forty dentin specimens were randomly divided into five groups of; 1-no treatment (control), 2-specimens treated with 10% pure nano-HAp and 3, 4, 5 specimens treated with 1%, 2%, and 3% F doped 10% nano-HAp, respectively. To evaluate the effectiveness of the materials used; pH, FTIR, and scanning electron microscopy evaluations were performed before and after degredation in simulated body fluid. To determine cytotoxicity of the materials, MTT assay was performed. Statistical evaluations were performed with F and t tests. All of the nano-HAp materials used in this study built up an effective covering layer on the dentin surfaces even with plugs in tubules. It was found that this layer had also a resistance to degradation. None of the evaluated nano-HAp types were have toxicity. Fluoride doping showed a positive effect on physical and chemical stability until a critical value of 1% F . The all evaluated nano-HAp types may be effectively used in dentin hypersensitivity treatment. The formed nano-HAp layers were seem to resistant to hydrolic deletion. The pure and 1% F doped nano-HAp showed the highest biocompatibility thus it was assessed that pure and 1% F doped materials may be used as an active ingredient in dentin hypersensitivity agents.

show abstract

Section: Discussion Ssupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Dentinal tubule occluding capability of nano‐hydroxyapatite; The in‐vitro evaluation

Bağlar

Erdem

Doğan

et al. 2018

Microscopy Res & Technique

View full text Add to dashboard Cite

show abstract

“…O método de ação baseia-se na obliteração dos túbulos dentinários pela precipitação de fosfato de cálcio na superfície dentinária, sendo o cálcio um componente frequentemente presente nos dentifrícios. Outros agentes dessensibilizantes como biocompósitos à base de sílica, cloreto de estrôncio e o nitrato de potássio também são utilizados 7 . O aumento da concentração do potássio extracelular ao redor das fibras nervosas leva à despolarização das mesmas e impede sua repolarização, resultando na inativação do potencial de ação e evitando, desta maneira, a geração da sintomatologia dolorosa 2,6 .…”

Section: Introductionunclassified

A utilização da laserterapia para o tratamento da hipersensibilidade dentinária

Costa¹,

Cury²,

Oliveira³

et al. 2016

J Health Sci

View full text Add to dashboard Cite

ResumoA hipersensibilidade dentinária -HD é caracterizada por uma dor aguda, de curta duração, causada pela movimentação de fluidos no interior de túbulos dentinários expostos ao meio bucal. Recentemente, a utilização dos lasers de alta e baixa intensidade foi proposta como uma alternativa para o tratamento da HD. Este trabalho realizou uma revisão da literatura baseada em artigos publicados entre 2009 e 2016 sobre os efeitos dos lasers de baixa e alta intensidade para o tratamento da HD. A base de dados utilizada foi o PubMed utilizando-se os descritores: Dentin hypersensitivity, laser, Nd:YAG, Er:YAG, GaAlAs, Er,Cr:YSGG, CO 2 , HeNe, e hydrodinamic theory. Foi observado que o tratamento da HD com lasers é um método biocompatível, não-invasivo e eficiente. Os lasers de baixa intensidade (HeNe e GaAlAs) estimulam as células nervosas do tecido pulpar, interferindo na polaridade das membranas celulares, pelo aumento da amplitude do potencial de ação. Esta ação leva ao bloqueio da transmissão do estímulo ao sistema nervoso central e estimula os odontoblastos a produzirem dentina reparadora, o que diminui a permeabilidade da dentina e interrompe a movimentação de fluidos nos túbulos dentinários. Os lasers em alta intensidade (Nd:YAG, Er:YAG, Er,Cr:YSGG e CO 2 ) têm o objetivo de obliterar a embocadura dos túbulos dentinários, através da irradiação direta da dentina exposta ao meio bucal, promovendo a dissolução e a ressolidificação dos cristais de hidroxiapatita da dentina, levando à eliminação da sensibilidade dolorosa por um tempo prolongado. Pode-se concluir que os lasers de alta e baixa intensidade são efetivos para o tratamento da hipersensibilidade dentinária cervical. Palavras IntroduçãoA dentina é um tecido duro, elástico e avascular que envolve a câmara pulpar. Ela é considerada um tecido heterogêneo, pois é composta por, aproximadamente, 70% de material inorgânico, 20% de material orgânico e 10% de água (em peso). Sua porção inorgânica é formada, principalmente, de cristais de hidroxiapatita, enquanto que sua fase orgânica é constituída pelas fibrilas de colágeno. A dentina é caracterizada pela presença de inúmeros túbulos dentinários, preenchidos pelo fluido dentinário, que se estendem desde a junção amelodentinária até a polpa 1 . Em condições normais, a dentina é recoberta pelo esmalte dental na sua porção coronária e pelo cemento na sua porção radicular.O aumento da expectativa de vida, associado à crescente difusão de princípios odontológicos preventivos tem contribuído para a manutenção de um maior número dentes nos arcos de indivíduos adultos. Essa longevidade aumentada vem acompanhada por um aumento da frequência de superfícies radiculares expostas, seja ela decorrente de tratamentos ortodônticos e periodontais, lesões cervicais não-cariosas, técnica incorreta de escovação, utilização de dentifrícios abrasivos, desordens oclusais, dentre outros. Esses fatores fazem com que a dentina fique exposta ao meio bucal tornando o dente sensível a estímulos táteis, térmicos químicos e osmóticos evaporativo,...

show abstract

The durability of resin–dentine bonds are enhanced by epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica

et al. 2022

View full text Add to dashboard Cite

Biomimetic nanohydroxyapatite (nHAp) has long been used as a biocompatible material for bone repair, bone regeneration, and bone reconstruction due to its low toxicity to local or systemic tissues. Various cross‐linkers have been employed to maintain the structure of collagen; these include epigallocatechin‐3‐gallate (EGCG), which can fortify the mechanical properties of collagen and withstand the degradation of collagenase. We hypothesized that EGCG combined with nHAp may promote resin–dentin bonding durability. Here, we examined the effect of epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica (EGCG@nHAp@MSN) on thermal stability and remineralization capability of dentin collagen. Dentin slices (2 × 2 × 1 mm3) were obtained and completely demineralized in a 10% phosphoric acid water solution. The resulting dentin collagen matrix was incubated with deionized water, EGCG, nHAp@MSN, and EGCG@nHAp@MSN. The collagen thermal degradation temperature was assessed utilizing differential scanning calorimetry analysis, which indicated that EGCG, nHAp@MSN, and EGCG@nHAp@MSN reinforced collagen's capability to resist thermal degradation. EGCG@nHAp@MSN resulted in the highest increase in denaturation temperature. Thermogravimetric analysis showed that both nHAp@MSN and EGCG@nHAp@MSN achieved a higher residual mass than the EGCG and control groups. Fourier transform infrared spectroscopy was performed to examine the interaction between EGCG@nHAp@MSN and dentin collagen. The EGCG@nHAp@MSN sample exhibited stronger dentin microhardness and uppermost bond strength after thermocycling. EGCG significantly enhanced collagen's capability to resist thermal degradation. In summary, EGCG and nHAp@MSN may work together to assist the exposed collagen to improve resistance to thermal cycling and promote remineralization while also strengthening the durability of resin–dentin bonds.

show abstract

A novel application of nanohydroxyapatite/mesoporous silica biocomposite on treating dentin hypersensitivity: An in vitro study

Cited by 62 publications

References 40 publications

Dentinal tubule occluding capability of nano‐hydroxyapatite; The in‐vitro evaluation

Dentinal tubule occluding capability of nano‐hydroxyapatite; The in‐vitro evaluation

A utilização da laserterapia para o tratamento da hipersensibilidade dentinária

The durability of resin–dentine bonds are enhanced by epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica

Contact Info

Product

Resources

About